Mutagenic and genotoxic effects of Guelma’s urban wastewater, Algeria

Abstract

Assessment of water pollution and its effect upon river biotic communities and human health is indispensable to develop control and management strategies. In this study, the mutagenicity and genotoxicity of urban wastewater of the city of Guelma in Algeria were examined between April 2012 and April 2013. For this, two biological tests, namely Amesand chromosomal aberrations (CA) test in Allium cepa root tips were employed on the samples collected from five different sampling stages (S1-S5). In Ames test, two strains of Salmonella typhimurium TA98 and TA100 with or without metabolic activation (S9-mix) were used. All water samples were found to be mutagenic to S. typhimurium TA98 with or without S9-mix. A significant decrease in mitotic index (MI) was observed with a decrease in the percentage of cells in the prophase and an increase in the telophase. Main aberrations observed were anaphase bridges, disturbed anaphase-telophase cells, vagrants and stickiness in anaphase-telophase cells. All treatments of wastewater in April 2012, at S5 in July 2012, at S1 and S5 in November 2012, at S5 in February 2013, and at S1 in April 2013 induced CA when compared to the negative control. Some physicochemical parameters and heavy metals (Cd, Pb, and Cu) were also recorded in the samples examined.

Feasibility of sand filters to wastewater treatment in rural areas in Algeria: experimental study

“…Some 11.2% of the volume is released into the subsoil via the traditional pits and 7.3% undergoes pre-treatment before its discharge into water streams (Table 2). These results confirmed other studies on pollution of river waters in Algeria (Badri et al, 2012;Guettaf et al, 2017;Tabet et al, 2015). The results obtained during this study showed that the majority of groundwater contamination by sewage in the river infiltration process depends on several environmental and climate factors (Kendouci et al, 2013).…”

“…In Algeria, the pollution of surface water has been demonstrated through several researches. One can cite as an example, the work of Tabet et al (2015) for the river of Seybouse (North-East of Algeria). He shows the presence of mutagenic in the river (salmonella typhimurium).…”

Bioassays based on higher plants as excellent dosimeters for ecotoxicity monitoring: A review

“…The herbicide caused a significant increase in the number of nucleoli and MN. Tabet et al (2015) examined the mutagenicity and genotoxicity of urban wastewater, Guelma city, Algeria between April 2012 and April 2013 using A. cepa CA and MI end points. The samples were collected from five different sampling stages (S1-S5).…”

Avaliação genotóxica e mutagênica de amostras de efluentes tratados por lagoas de estabilização em Teresina-Piauí

“…Os controles positivo e negativo apresentaram resultados esperados e, portanto, validam os resultados obtidos em testes com as amostras. Nossos resultados são consistentes com os resultados encontrados na literatura mostrando a relação entre poluição urbana e aumento de danos no DNA (BIANCHI et al, 2011;GEREMIAS et al, 2012;TABET et al, 2015) Observou-se a ação de toxicidade pela redução do índice mitótico (IM) e a redução no tamanho das raízes em todos os testes, nas diferentes amostras coletadas durante todo o período seco. Percebeu-se que comparado ao controle positivo (CP) para o IM, os valores mais significativos foram dos pontos, P1 e P2 durante o período seco.…”