Mutagenic and recombinogenic action of pesticides in Aspergillus nidulans

Bignami, Margherita; Aulicino, Francesca Anna; Velcich, Anna; Carere, A.; Morpurgo, G.

doi:10.1016/0165-1161(77)90017-6

Cited by 75 publications

(10 citation statements)

References 22 publications

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“…Conclusive evidence that this protein was tubulin has been presented by Sheir-Neiss et al (50); they demonstrated that beno- The primary genetic effect of MBC has been claimed to be chromosome nondisjunction, seen as an increased frequency of mitotic segregation for heterozygous markers (1,21,38). However, a number of other genetic effects have also been observed.…”

mentioning

confidence: 98%

Genetic effects of methyl benzimidazole-2-yl-carbamate on Saccharomyces cerevisiae.

Wood¹

1982

Mol. Cell. Biol.

100

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The genetic effects of the mitotic inhibitor methyl benzimidazole-2-yl-carbamate (MBC) have been studied in Saccharomyces cerevisiae. MBC had little or no effect on the frequency of mutation. In some experiments MBC caused an increase in the frequency of mitotic recombination; however, this effect was small and not reproducible. The primary genetic effect of MBC was to induce mitotic chromosome loss at a high frequency. Chromosome loss occurred at equal frequencies for all chromosomes tested (13 of 16). Cells which had lost multiple chromosomes were found more frequently than predicted if individual chromosome loss events were independent. The probability of loss for a particular chromosome increased with length of time cells were incubated with MBC. MBC treatment also increased the frequency at which polyploid cells were found. These results suggested that MBC acted to disrupt the structure or function of the mitotic spindle and cause chromosome nondisjunction.

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confidence: 98%

Genetic effects of methyl benzimidazole-2-yl-carbamate on Saccharomyces cerevisiae.

Wood¹

1982

Mol. Cell. Biol.

100

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“…They think that benomyl is a novel base analogue mutagen that, when incorporated into DNA, is detected by UV endonuclease and provokes mutations not by misincorpation during normal DNA replication, but by misrepair of gaps in newly-synthesized DNA. SEILER (1973) found benomyl to increase muta- KAPPAS et al (1974);by BIGNAMI et al (1977); and by KAPPAS (1978). They all found benomyl to be active in causing mitotic non-disjunction.…”

Section: Resultsmentioning

confidence: 99%

Benomyl-An investigation of its mutagenic effect in bacteriophage T4D

Kvelland

2008

Hereditas

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“…I might point out that benomyl, the active ingredient in Benalte, has been shown to induce nondisjunction in fungal test systems (15,16). This same chemical is utilized as a fungicide on soybeans, and a large portion of the soybeans raised for seed production in the United States are treated with this chemical.…”

Section: Test System Threementioning

confidence: 99%

Maize Pollen Test Systems to Detect Nondisjunction

Weber¹

1981

Environmental Health Perspectives

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. The National Institute of Environmental Health Sciences (NIEHS) and Brogan & Partners are collaborating with JSTOR to digitize, preserve and extend access to Environmental Health Perspectives.Three pollen test systems to detect the induction of nondisjuction in maize are actively being explored. (1) Each member of a tetrad of haploid microspores produced by meiosis contains a single chromosome 6 which carries the only nucleolar organizing region in the maize genome. Thus, each member of a normal tetrad contains one nucleolus. If nondisjunction took place at the first or second meiotic division, 2:2:0:0 or 2:1:1:0 tetrads would be produced respectively.(2) If a male parent carrying a dominant endosperm marker is crossed by a female carrying a recessive allele of this gene, all normal kernels would be heterozygous and would express the dominant phenotype in their endosperm. If nondisjunction of the chromosome carrying this gene took place at the second microspore division and the nullisomic and disomic sperm fertilized the polar nuclei and egg of a given embryo sac respectively, an exceptional kernel is produced which would express the recessive endosperm phenotype and contain a trisomic embryo. (3) Complementing null mutations of genes expressed in individual pollen grains can be utilized to detect nondisjunction. Normal haploid pollen grains from plants heterozygous for two complementing null alleles of a locus would each express the recessive phenotype. If nondisjunction took place during either meiotic division, disomic pollen grains containing both alleles could be produced expressing the dominant phenotype due to complementation. We are exploring this test system utilizing appropriate alcohol dehydrogenase mutants.

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Mutagenic and recombinogenic action of pesticides in Aspergillus nidulans

Cited by 75 publications

References 22 publications

Genetic effects of methyl benzimidazole-2-yl-carbamate on Saccharomyces cerevisiae.

Genetic effects of methyl benzimidazole-2-yl-carbamate on Saccharomyces cerevisiae.

Benomyl-An investigation of its mutagenic effect in bacteriophage T4D

Maize Pollen Test Systems to Detect Nondisjunction

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