Mutagenic effect of N-Methyl-N-nitrosourea onMycobacterium phlei

Gene manipulation in mycobacteria developed in two phases. In the first phase genes of mycobacteria were transferred into cells of E. coli and Streptomyces lividans. In the second phase, heterologous genes were transferred into mycobacteria either with a shuttle plasmid or hybrid plasmids. A prerequisite for successful gene manipulation in mycobacteria was a thorough understanding of plasmids in mycobacteria. Construction of recombinant DNA molecules contributed not only to the fact that mycobacteria did not remain outside the mainstream of modern genetic research but also to their present practical importance.

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Gene manipulation in mycobacteria

Koníčék

Koníčková-Radochová

Slosárek

1991

Folia Microbiol

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Pigmentation changes in Brevibecteria induced by mutagenic treatment

Koníčková-Radochová¹,

Koníčék

Rytíř

1988

Folia Microbiol

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Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three new pigmentation types, viz. white, pink and orange, were observed. The yellow pigmented parent strains are most resistant to the lethal effects of UV radiation. By selecting pigmented mutants of all types on media containing antibiotics it was possible to obtain strains that were resistant either to tetracycline or to streptomycin. Auxotrophic pigmented mutants were also isolated. In multiple mutant strains of Brevibacterium sp. M27 a number of strainsexhibited a changed L-lysine production. In some strains the production was variable, whereasother strains did not produce L-lysine at all and stains with a limited production of other amino acids were also detected.

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Mutagenic effect of N-Methyl-N-nitrosourea onMycobacterium phlei

Cited by 2 publications

References 8 publications

Gene manipulation in mycobacteria

Gene manipulation in mycobacteria

Pigmentation changes in Brevibecteria induced by mutagenic treatment

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