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Of several chemicals tested on the elimination of plasmids from Escherichia coli K-12, the compound designated ICR-170 was most effective, applied at 100 mu g/ml, the effect being comparable to that of acriflavin. It had no effect on the elimination of the R1 plasmid from Escherichia coli JC 5455.

Four non-transformable mutants of Bacillus subtilis 168 defective in the penetration of DNA into the recipient cell were isolated. All mutants were fully non-transformable with mutation in genes influencing irreversible binding of the donor DNA by the recipient cell.