Non-transformable mutants ofBacillus subtilis defective in the penetration of DNA into the cell

Transformation-deficient mutants of Bacillus subtilis have been identified either by screening for a nuclease-deficient phenotype on methyl green-DNA agar or for nontransformability on transforming DNA-containing agar. After purification of the mutations causing a reduction in the entry of DNA, a set of isogenic entrydeficient strains was obtained. In addition to being entry deficient to various extents, the strains usually were less capable of association with DNA than the entry-proficient parent. Likewise, the specific transforming activity in the purified mutant strains continued to be less than that in the wild type. With the possible exception of one strain, no evidence was obtained that the mutant strains were impaired in recombination. Since the breakdown of transforming DNA to acidsoluble products correlated fairly well with the residual capacity of the strains to take up DNA, nucleolytic activity is likely to be involved in the entry of DNA in B. subtilis.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Non-transformable mutants ofBacillus subtilis defective in the penetration of DNA into the cell

Abstract: Four non-transformable mutants of Bacillus subtilis 168 defective in the penetration of DNA into the recipient cell were isolated. All mutants were fully non-transformable with mutation in genes influencing irreversible binding of the donor DNA by the recipient cell.

Cited by 2 publications

References 32 publications

Transformation of protoplasts of nontransformableBacillus subtilis mutants by plasmid pUB 110 DNA

Transformation of protoplasts of nontransformableBacillus subtilis mutants by plasmid pUB 110 DNA

Isolation and partial characterization of Bacillus subtilis mutants impaired in DNA entry

Contact Info

Product

Resources

About