Mutagenic effects of carbon ions near the range end in plants

Hase, Yoshihiro; Yoshihara, Ryouhei; Nozawa, Shigeki; Narumi, Issay

doi:10.1016/j.mrfmmm.2011.10.004

Cited by 39 publications

(37 citation statements)

References 42 publications

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“…This phenomenon possibly correlates with an inefficiency of the C-NHEJ pathway at DSBs induced by high-LET radiation (Wang et al, 2008(Wang et al, , 2010. In support of this theory, it was found that an A. thaliana DNA ligase IV mutant, deficient in the C-NHEJ pathway, was more sensitive to 113 keV lm À1 C-ion irradiation than to 425 keV lm À1 compared with the wild type (Hase et al, 2012). This finding raises the possibility that C-NHEJ in A. thaliana is inherently inefficient at repairing the DSBs induced by higher-LET radiation, as produced by a 425 keV lm À1 C-ion beam, although the sensitivity of the homologous recombination pathway to high-LET radiation has to be considered in the ligase IV mutant as well.…”

Section: Discussionmentioning

confidence: 87%

Comprehensive identification of mutations induced by heavy‐ion beam irradiation in Arabidopsis thaliana

et al. 2015

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SUMMARYHeavy-ion beams are widely used for mutation breeding and molecular biology. Although the mutagenic effects of heavy-ion beam irradiation have been characterized by sequence analysis of some restricted chromosomal regions or loci, there have been no evaluations at the whole-genome level or of the detailed genomic rearrangements in the mutant genomes. In this study, using array comparative genomic hybridization (array-CGH) and resequencing, we comprehensively characterized the mutations in Arabidopsis thaliana genomes irradiated with Ar or Fe ions. We subsequently used this information to investigate the mutagenic effects of the heavy-ion beams. Array-CGH demonstrated that the average number of deleted areas per genome were 1.9 and 3.7 following Ar-ion and Fe-ion irradiation, respectively, with deletion sizes ranging from 149 to 602 180 bp; 81% of the deletions were accompanied by genomic rearrangements. To provide a further detailed analysis, the genomes of the mutants induced by Ar-ion beam irradiation were resequenced, and total mutations, including base substitutions, duplications, in/dels, inversions, and translocations, were detected using three algorithms. All three resequenced mutants had genomic rearrangements. Of the 22 DNA fragments that contributed to the rearrangements, 19 fragments were responsible for the intrachromosomal rearrangements, and multiple rearrangements were formed in the localized regions of the chromosomes. The interchromosomal rearrangements were detected in the multiply rearranged regions. These results indicate that the heavy-ion beams led to clustered DNA damage in the chromosome, and that they have great potential to induce complicated intrachromosomal rearrangements. Heavy-ion beams will prove useful as unique mutagens for plant breeding and the establishment of mutant lines.

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Section: Discussionmentioning

confidence: 87%

Comprehensive identification of mutations induced by heavy‐ion beam irradiation in Arabidopsis thaliana

et al. 2015

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“…Three replications of more than 25 seeds were used for each dose. Survival curves were drawn on the basis of the single hit-multitarget theory using the following equation as previously described (Hase et al, 2012):…”

Section: Acute Gamma Irradiationmentioning

confidence: 99%

Genetic Consequences of Acute/Chronic Gamma and Carbon Ion Irradiation of Arabidopsis thaliana

et al. 2020

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Gamma rays are the most frequently used ionizing radiation in plant mutagenesis; however, few studies are available on the characteristics of mutations at a genome-wide level. Here, we quantitatively and qualitatively characterized the mutations induced by acute/chronic gamma ray irradiation in Arabidopsis. The data were then compared with those previously obtained for carbon ion irradiation. In the acute irradiation of dry seeds at the same effective survival dose, gamma rays and carbon ions differed substantially, with the former inducing a significantly greater number of total mutation events, while the number of gene-affecting mutation events did not differ between the treatments. This may result from the gamma rays predominantly inducing single-base substitutions, while carbon ions frequently induced deletions ≥2 bp. Mutation accumulation lines prepared by chronic gamma irradiation with 100-500 mGy/h in five successive generations showed higher mutation frequencies per dose compared with acute irradiation of dry seeds. Chronic gamma ray irradiation may induce larger genetic changes compared with acute gamma ray irradiation. In addition, the transition/transversion ratio decreased as the dose rate increased, suggesting that plants responded to very low dose rates of gamma rays (∼1 mGy/h), even though the overall mutation frequency did not increase. These data will aid our understanding of the effects of radiation types and be useful in selecting suitable radiation treatments for mutagenesis.

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“…In tabaco single cells, initial yields of DNA double-strand breaks (DSBs) by the different LET (94.8 to 431 keV/µm) of carbon ion beams depended on LET as well as the RBE based on the lethality, and the highest DSBs yields were obtained at 124 and 241 keV/µm [67]. In A. thaliana, it has been shown that carbon ions near the range end (425 keV/µm) frequently cause large-scale deletions compared with carbon ions penetrating the seeds (113 keV/µm) [68]. Moreover, in rice, the mutation frequency by the two different LET carbon ion beams (76 and 107 keV/µm) has been investigated [69].…”

Section: Discussionmentioning

confidence: 99%

Studies on Application of Ion Beam Breeding to Industrial Microorganisms at TIARA

Satoh

Oono

2019

QuBS

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Mutation-breeding technologies are useful tools for the development of new biological resources in plants and microorganisms. In Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) at the National Institutes for Quantum and Radiological Science and Technology, Japan, ion beams were explored as novel mutagens. The mutagenic effects of various ion beams on eukaryotic and prokaryotic microorganisms were described and their application in breeding technology for industrial microorganisms were discussed. Generally, the relative biological effectiveness (RBE) depended on the liner energy transfer (LET) and the highest RBE values were obtained with 12C5+ ion beams. The highest mutation frequencies were obtained at radiation doses that gave 1%–10% of surviving fraction. By using 12C5+ ion beams in this dose range, many microorganisms have been improved successfully at TIARA. Therefore, ion-beam breeding technology for microorganisms will have applications in many industries, including stable food production, sustainable agriculture, environmental conservation, and development of energy resources in the near future. Moreover, genome analyses of the ion-beam-induced mutants are in progress to clear the differences of mutational functions induced by different LET radiations in microorganisms. Further characterizations of mutations induced by different LET radiations will facilitate more effective use of ion beams in microorganisms breeding.

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Mutagenic effects of carbon ions near the range end in plants

Cited by 39 publications

References 42 publications

Comprehensive identification of mutations induced by heavy‐ion beam irradiation in Arabidopsis thaliana

Comprehensive identification of mutations induced by heavy‐ion beam irradiation in Arabidopsis thaliana

Genetic Consequences of Acute/Chronic Gamma and Carbon Ion Irradiation of Arabidopsis thaliana

Studies on Application of Ion Beam Breeding to Industrial Microorganisms at TIARA

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