Mutagenic potentials of dental cements as detected by the Salmonella/microsome test

Kaplan, Çiǧdem; Dırıl, Nuran; Sahin, Şevki; Çehreli, Murat Cavit

doi:10.1016/j.biomaterials.2003.10.101

Cited by 20 publications

(16 citation statements)

References 25 publications

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“…42 Salmonella typhimurium was tested for evaluation of mutagenic capacity of some dental materials. 43 The antibacterial activity of neat membrane (A0b20) and AMX-loaded membranes (A1 and A1.5 series) are presented in Table II and Figure S1 (Supporting Information). As expected, membranes with 1.5 wt % AMX showed higher antimicrobial activity than those with 1 wt % AMX.…”

Section: Microbiological Evaluation Of Antibacterial Activity Of Membmentioning

confidence: 99%

Amoxicillin-loaded electrospun nanocomposite membranes for dental applications

Furtos

Rivero

Răpuntean

et al. 2016

J. Biomed. Mater. Res.

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Electrospun nanocomposite matrices based on poly(ε-caprolactone) (PCL), nano-hydroxyapatite (nHAp) and amoxicillin (AMX) were designed and investigated for dental applications. nHAp provides good biocompatibility, bioactivity, osteoconductivity, and osteoinductivity properties, and AMX, as antibiotic model, controls and/or reduces bacterial contamination of periodontal defects while enhancing tissue regeneration. A series of polymeric nanocomposites was obtained by varying both the antibiotic and nHAp contents. Fibrous membranes of different compositions were obtained by electrospinning technique, and morphological, thermal, mechanical and surface properties were characterized. The incorporation of AMX seemed to alter the nHAp distribution within the microfibrous matrix. The interaction between AMX and nHAp affected the mechanical performance and modulated the antibiotic release behavior. AMX release profiles presented a burst release that depended on nHAp content, followed by a slow release stage where the drug content (85-100%) was released in 3 weeks. The antimicrobial activity of the AMX-loaded membranes was tested with four bacterial strains depended on both the drug and nHAp contents. Extensive mineralization in simulated body fluid (SBF) was evidenced by SEM/EDX analysis after 21 days. The studied electrospun nanocomposite amoxicillin-loaded membranes could be a promising fibrous-based antibiotic carrier system for dental and tissue engineering applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 966-976, 2017.

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Section: Microbiological Evaluation Of Antibacterial Activity Of Membmentioning

confidence: 99%

Amoxicillin-loaded electrospun nanocomposite membranes for dental applications

Furtos

Rivero

Răpuntean

et al. 2016

J. Biomed. Mater. Res.

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“…Although the Ames test is a well-established method with high sensitivity, mutagenicity tests may occasionally exhibit false-positive results and it is not possible to draw a conclusive statement based solely on a single study (Maslat et al 2002;Kaplan et al 2004). In this connection, the imidazole derivatives should be more investigated in subsequent studies in order to compare the results from different test systems before they are considered as pro-drug molecules.…”

Section: Resultsmentioning

confidence: 99%

“…The continuing popularity of the Salmonella mutagenicity assay depends on the ability of prescreening a large number of chemicals for the identification of genotoxic hazards and the elucidation of the biochemical mechanisms of mutagenesis (Mamber et al 1993;Josephy et al 1997). In addition, the test is used for its high sensitivity and recognized validity (Kaplan et al 2004). In the present study, the compounds were tested on Salmonella typhimurium strains TA98 and TA100 using the standard plate incorporation procedure in the presence and absence of metabolic activation.…”

Section: Introductionmentioning

confidence: 99%

Mutagenic activities of ten imidazole derivatives in Salmonella typhimurium

Aydoğan

Kutlu

2007

Biologia

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Ten imidazole derivatives were tested for mutagenicity in Salmonella typhimurium strains TA98 and TA100 both in the absence and presence of metabolic activation by the microsomal fraction S9 mix. In a general manner, derivatives tested exhibited a greater mutagenic activity in the TA100 strain comparing to the responses in TA 98. In the standard plate incorporation assay, 8 of these substances (80%) were found to be mutagenic for at least one of the two strains in the presence or absence of metabolic activation. Two compounds showed positive results in TA98 and 6 compounds were also mutagenic in TA100 without S9. In the presence of S9 mix, all of the 10 substances were non-mutagenic in TA98, whereas 4 compounds were positive in TA100. The results suggested the mutagenic potentials of the imidazole derivatives particularly inducing the reversion of base-pair substitutions. According to the structure-activity relationships phenyl groups in position 2 with different substituents can confer the mutagenic activity of the tested compounds. Methyl groups in different positions of these phenyl substituents can cause different types of mutations. This mutagenic effect is observed more clearly when the phenyl group is inhibited with a nitro group.

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“…The increased frequency of micronuclei found in the Ca 3 SiO 5 group in our study may be related to a chemical compound present in the powder and/or in the liquid component of Ca 3 SiO 5 that is not present in MTA. One study demonstrated that polycarboxylate (Aqualox ® ) had a mutagenic effect in an assay using S. typhimurium TA 98 and TA 1535 27 . This finding supports the hypothesis that the increased frequency of micronuclei observed after exposure to Ca 3 SiO 5 may be related to compounds in the liquid component, such as polycarboxylate.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

et al. 2016

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Ca 3 SiO 5 is new cement based on the composition of Portland that has been developed to have superior physicochemical and biological properties. In a clinical evaluation, the cement did not appear to have cytotoxic properties and allowed for the proliferation of pulp cells and gingival fibroblasts. However, no previous studies have evaluated the genotoxicity or the mutagenicity of Ca 3 SiO 5 in vivo. Therefore, the goal of this study is to evaluate the genotoxic and mutagenic potential of Ca 3 SiO 5 -based cement in vivo. Twenty-four male Wistar rats were divided into 3 groups (n = 8). Group A rats received subcutaneous implantation of Ca 3 SiO 5 in the dorsum. Group B rats received a single dose of cyclophosphamide (positive control). Group C rats received subcutaneous implantation of empty tubes in the dorsum (negative control). After 24 hours, all animals were euthanized and the bone marrow of the femurs was collected for use in the comet assay and the micronucleus test. The comet assay revealed that the Ca 3 SiO 5 group had a tail intensity of 23.57 ± 7.70%, the cyclophosphamide group had a tail intensity of 27.43 ± 7.40%, and the negative control group had a tail intensity of 24.75 ± 5.55%. The average number of micronuclei was 6.25 (standard deviation, SD = 3.53) in the Ca 3 SiO 5 group, 9.75 (SD = 2.49) in the cyclophosphamide group, and 0.75 (SD = 1.03) in the negative control group. There was an increase in the micronuclei frequency in the Ca 3 SiO 5 group compared to that of the negative control group (p < 0.05). Our data showed that exposure to the Ca 3 SiO 5 -based cement resulted in an increase in the frequency of micronuclei, but no genotoxicity was detected according to the comet assay.

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Mutagenic potentials of dental cements as detected by the Salmonella/microsome test

Cited by 20 publications

References 25 publications

Amoxicillin-loaded electrospun nanocomposite membranes for dental applications

Amoxicillin-loaded electrospun nanocomposite membranes for dental applications

Mutagenic activities of ten imidazole derivatives in Salmonella typhimurium

Evaluation of the genotoxicity and mutagenicity of Ca3SiO5-based cement

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