Mutagenicity of acridines in a reversion assay based on tetracycline resistance in plasmid pBR322 in Escherichia coli

Hoffman, George R.; Deschênes, Suzanne M.; Manyin, Teresa; Fuchs, Robert P. P.

doi:10.1016/0027-5107(95)00206-5

Cited by 22 publications

(24 citation statements)

References 29 publications

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“…Nevertheless, the lacZ reversion assay (Table 1, Fig. 2), a reversion assay in the tetracycline resistance gene (tet) in pBR322 [Hoffmann et al, 1996], and sequence data for forward mutations in the cl gene of phage [Skopek and Hutchinson, 1984] present a consistent pattern of 9AA most efficiently inducing ϪG frameshifts. Quinacrine was widely used historically as an antimalarial drug, has found use in treating other diseases, and is now used in women for voluntary nonsurgical sterilization [Benagiano, 2001;Clarke et al, 2001].…”

Section: Discussionmentioning

confidence: 94%

“…There is a predominance of ϩG frameshifts among mutations induced by ICR-191 in the lacZ assay (Table III, Fig. 2), as well as in the lacI [Calos and Miller, 1981], pBR322 tet [Hoffmann et al, 1996], and cI assays [Skopek and a Analysis of variance shows highly significant (P Ͻ 0.01) increases in revertants per plate and in revertant frequencies in all five strains. Hutchinson, 1984].…”

Section: Discussionmentioning

confidence: 97%

“…It is likely, but not certain, that the same would apply to hisD3052 revertants induced by Entozon. Entozon induced Ϫ2 frameshifts effectively in a reversion assay in the tet gene of pBR322 [Hoffmann et al, 1996].…”

Section: Discussionmentioning

confidence: 99%

“…Their structures are shown in Figure 1. The data permit comparisons to a reversion assay in the tetracycline resistance (tet) gene of pBR322 [Hoffmann et al, 1996] and to results for 9AA and ICR-191 in the lacZ assay in other laboratories using different procedures. They also show that Entozon, unlike simple intercalators, is a potent inducer of Ϫ2 frameshifts and that quinacrine, although a simple intercalator, induces all five kinds of frameshift mutations detected by the assay.…”

Section: Introductionmentioning

confidence: 98%

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Frameshift mutations induced by three classes of acridines in the lacZ reversion assay in Escherichia coli: Potency of responses and relationship to slipped mispairing models

Hoffmann

Calciano

Lawless

et al. 2003

Environ and Mol Mutagen

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The frameshift mutagenicity of 9-aminoacridine (9AA) was compared with that of quinacrine, the acridine mustards ICR-191 and quinacrine mustard (QM), and the nitroacridine Entozon in the lacZ reversion assay in Escherichia coli. As intercalating agents, 9AA and quinacrine cause mutations through noncovalent associations with DNA. Mustards and nitroacridines form covalent adducts in DNA and give rise to different spectra of mutations. Quinacrine and 9AA most effectively induced -1 frameshifts in a run of guanine residues, with 9AA being the more potent mutagen. They also induced +G frameshifts. The acridine mustard ICR-191 was a stronger mutagen than 9AA, owing largely to its potent induction of +G frameshifts. QM induced +G frameshifts more strongly than did its nonreactive counterpart quinacrine. The nitroacridine Entozon differed from the other acridines in being a potent inducer of -2 frameshifts, but it was less effective in inducing +/-1 frameshifts. Quinacrine, although a simple intercalator, induced all five kinds of frameshift mutations detected in the assay, as did the acridine mustards. Although +A and -A frameshifts were induced, adenine runs were less susceptible to acridine mutagenesis than guanine runs. The patterns of frameshift mutagenicity in the lacZ assay are similar to those in an assay based on the reversion of mutations in the tetracycline-resistance gene of the plasmid pBR322. The similarity suggests that the responses reflect the inherent bacterial mutagenicity of the compounds in the local sequence context and are not highly dependent on the broader sequence context. The results are interpreted with respect to slipped mispairing models of frameshift mutagenesis.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Frameshift mutations induced by three classes of acridines in the lacZ reversion assay in Escherichia coli: Potency of responses and relationship to slipped mispairing models

Hoffmann

Calciano

Lawless

et al. 2003

Environ and Mol Mutagen

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“…This effect has been shown in many eukaryotic cells, including viruses, bacteria, and various cancer cells (8,9). Apart from this important feature, chloroquine, a necrosis factor, acts almost like an immunomodulatory by inhibiting phospholipase A2 and tumors (10).…”

Section: Discussionmentioning

confidence: 99%

The effect of chloroquine treatment in malignant astrocytomas on prognosis

Bulut¹,

Ekici²,

Tucer³

et al. 2013

Turk J Med Sci

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A series of new 4-(3,4-dimethoxyphenethyl)-5-akyl/aryl-2H-1,2,4-triazol-3(4H)-ones (3a-g) was obtained by the reaction of ethyl 2-(ethoxy)(alkylidene/arylidene)hydrazinecarboxylate (1) and 2-(3,4-dimethoxyphenyl)ethanamine (2). Compounds 4a-f and 5 were synthesized from the reaction of corresponding compounds 3a-f and 3g with BBr 3 , respectively. With elemental analysis, IR, 1 H-NMR, and 13 C-NMR spectral data, 14 newly synthesized compounds were characterized. The structure of compound 3a was inferred through IR, 1 H-and 13 C-NMR, elemental analysis, and X-ray spectral techniques. In addition, the newly synthesized chemicals were screened for their antioxidant properties. Among the chemicals tested, 4a, 4c, 4d, 4f, and 5 exhibited the highest degree of antioxidant activity.

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Preparation of poly(9‐aminoacridine)‐modified electrode and its application in the determination of dopamine and ascorbic acid simultaneously

Fang

Liu

Wang

et al. 2007

J of Applied Polymer Sci

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A novel modified electrode was fabricated with 9‐aminoacridine by electropolymerization in the phosphate buffer solution (PBS) (pH 7.4) and was characterized by cyclic voltammetry (CV). The modified electrode showed excellent electrocatalytic effect and high stability toward the electrochemical oxidation of dopamine (DA) and ascorbic acid (AA). Also, it showed a high stability for the determination of DA and AA simultaneously. Well‐separated voltammetric peaks were observed for DA and AA on the modified electrode. The separation of two anodic peaks was 170 mV, which was large enough to eliminate the interference of AA and determine DA. The differential pulse voltammograms (DPV) were used for the measurement of DA by means of the poly(9‐aminoacridine)‐modified electrode in PBS at pH 7.4. A linear response toDA was observed in the concentration range from 1.5 × 10−6 to 3.5 × 10−3 mol L−1 with a correlation coefficient of 0.9998 and a detection limit (S/N = 3) of 1.0 × 10−7mol L−1. The proposed method was used to determine DA in DA‐hydrochloride injection and showed excellent sensitivity and recovery. The ease of fabrication, good reproducibility, high stability, and low cost of the modified electrode are the promising features of the proposed sensor. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 104: 3864–3870, 2007

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Mutagenicity of acridines in a reversion assay based on tetracycline resistance in plasmid pBR322 in Escherichia coli

Cited by 22 publications

References 29 publications

Frameshift mutations induced by three classes of acridines in the lacZ reversion assay in Escherichia coli: Potency of responses and relationship to slipped mispairing models

Frameshift mutations induced by three classes of acridines in the lacZ reversion assay in Escherichia coli: Potency of responses and relationship to slipped mispairing models

The effect of chloroquine treatment in malignant astrocytomas on prognosis

Preparation of poly(9‐aminoacridine)‐modified electrode and its application in the determination of dopamine and ascorbic acid simultaneously

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