Mutagenicity of p-aminophenol in E. coli WP2uvrA/pKM101 and its relevance to oxidative DNA damage

Yoshida, Rie; Oikawa, Shinji; Ogawa, Yasutaka; Miyakoshi, Yuichi; Ooida, Motoi; Asanuma, Kazunari; Shimizu, Hidesuke

doi:10.1016/s1383-5718(98)00058-8

Cited by 18 publications

(14 citation statements)

References 35 publications

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“…The present study showed follicular atresia this may be due to sodium nitrophenolates that disturbed the reproductive hormones, this result agreed with Koerdel et al [19][20][21][22][23][24][25] who reported that Phenols also affect the function of the hormonal system. Some phenols are capable of disturbing sexual hormones function, which finally may lead to sterility of animals and humans.…”

Section: Discussionsupporting

confidence: 93%

Effect of Long Term Administration of Atonik Compound in Female Rabbits on Hematological and Pathological Changes in Important Functional Body Organ

MJ¹,

Mohamed²,

Ghusoon³

2016

Anat Physiol

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Atonik is a synthetic bio stimulant consist of three phenolic compounds used as stimulant plant growth and development, farmers used atonik with high abuse for enhancements of fruit maturity so study done to detect the harmful effect of atonik in laboratory animal.Eight female rabbits of weighting (1.4-1.6) kg were obtained from animal house in college of Veterinary Medicine, animal housed two weeks before beginning study and all laboratory condition was taking into consideration. Animal randomly divided in to two main groups: Control group still without treatment at all period of study, Atonik group: each rabbits orally received 0.1 mg/kg /three time weekly (TIDW). Our result disturbance of hematological parameters specially WBCS, RBCS, HB and lymphocyte. Moreover histopathological section showing dilation of the central vein and filled with fluid and granuloma beside it, with vaculation of hypatocyte. Kidney reveals coagulative necrosis of renal tubules with distraction of glomerular tuft. Uterus of rabbits treated with 0.1 mg/kg bw atonik for 8 weeks showing necrosis of unrein glands. Ovary showed increase in the numbers of attratic follicles. Our result concluded that atonik has very harmful effect for reproduction, liver, and kidney.

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Section: Discussionsupporting

confidence: 93%

Effect of Long Term Administration of Atonik Compound in Female Rabbits on Hematological and Pathological Changes in Important Functional Body Organ

MJ¹,

Mohamed²,

Ghusoon³

2016

Anat Physiol

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“…It is highly toxic and mutagenic and induces DNA cleavage in mouse and human lymphoma cells (Majeska and Holden 1995;Yoshida et al 1998). 4-Aminophenol is degraded by only a few bacterial species (Takenaka et al 2003;Ahmed et al 2001).…”

Section: -Aminophenol Is An Intermediate In the Biodegradation Of Hymentioning

confidence: 99%

Fe-superoxide dismutase and 2-hydroxy-1,4-benzoquinone reductase preclude the auto-oxidation step in 4-aminophenol metabolism by Burkholderia sp. strain AK-5

et al. 2010

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Burkholderia sp. strain AK-5 converts 4-aminophenol to maleylacetic acid via 1,2,4-trihydroxybenzene, which is unstable in vitro and non-enzymatically auto-oxidized to 2-hydroxy-1,4-benzoquinone. Crude extract of strain AK-5 retarded the auto-oxidation and reduced the substrate analogue, 2,6-dimethoxy-1,4-benzoquinone, in the presence of NADH. The two enzymes responsible were purified to homogeneity. The deduced amino acid sequence of the enzyme that inhibited the auto-oxidation showed a high level of identity to sequences of iron-containing superoxide dismutases (Fe-SODs) and contained a conserved metal-ion-binding site; the purified enzyme showed superoxide dismutase activity and contained 1 mol of Fe per mol of enzyme, identifying it as Fe-SOD. Among three type SODs tested, Fe-SOD purified here inhibited the auto-oxidation most efficiently. The other purified enzyme showed a broad substrate specificity toward benzoquinones, including 2-hydroxy-1,4-benzoquinone, converting them to the corresponding 1,4-benzenediols; the enzyme was identified as 2-hydroxy-1,4-benzoquinone reductase. The deduced amino acid sequence did not show a high level of identity to that of benzoquinone reductases from bacteria and fungi that degrade chlorinated phenols or nitrophenols. The indirect role of Fe-SOD in 1,2,4-trihydroxybenzene metabolism is probably to scavenge and detoxify reactive species that promote the auto-oxidation of 1,2,4-trihydroxybenzene in vivo. The direct role of benzoquinone reductase would be to convert the auto-oxidation product back to 1,2,4-trihydroxybenzene. These two enzymes together with 1,2,4-trihydroxybenzene 1,2-dioxygenase convert 1,2,4-trihydroxybenzene to maleylacetic acid.

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“…The results showed that mannitol can inhibit the formation of 8-hydroxyguanine and reduce the level of it to a controllable extent [50], can restrain DNA bond breakage [51] and completely inhibit both p-aminophenol (p-AP) mediated DNA damages and . OH production by p-AP in the presence of Fe(III) [52].…”

Section: Effect Of Mannitol Concentration On the Dna Damagementioning

confidence: 99%

Electrochemical Study on DNA Damage Based on the Direct Oxidation of 8‐Hydroxydeoxyguanosine at an Electrochemically Modified Glassy Carbon Electrode

Zhang

Wang

et al. 2008

Electroanalysis

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The study of DNA damage induced by Fenton reaction (Fe 2þ /H 2 O 2 ) in vitro was performed based on the direct electrochemical oxidation of 8-hydroxydeoxyguanosine (8-OH-dG), the biomarker of DNA oxidative damage, at an electrochemically modified glassy carbon electrode (GCE). The effects of antioxidants, such as ascorbic acid, and hydroxyl-radical scavenger (mannitol) on the DNA damage were also investigated. 8-OH-dG, the oxidation product of guanine residues in DNA, has shown significantly oxidative peak on the electrochemically modified GCE. The oxidative peak current of 8-OH-dG was linear with the damaged DNA concentration in the range of 10 -200 mg/L. The experimental results demonstrate that ascorbic acid has ambivalent effect on DNA oxidative stress. It can promote DNA oxidative damage when ascorbic acid concentration is below 1.5 mM and protect DNA from damage in the range of 1.5 -2.5 mM. As a hydroxyl-radical scavenger, mannitol inhibits significantly DNA oxidative damage. The influence of Fe 2þ , as reactant, and EDTA as iron chelator in the system were also studied. The proposed electrochemical method can be used for the estimation of DNA oxidative damage from new point of view.

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Mutagenicity of p-aminophenol in E. coli WP2uvrA/pKM101 and its relevance to oxidative DNA damage

Cited by 18 publications

References 35 publications

Effect of Long Term Administration of Atonik Compound in Female Rabbits on Hematological and Pathological Changes in Important Functional Body Organ

Effect of Long Term Administration of Atonik Compound in Female Rabbits on Hematological and Pathological Changes in Important Functional Body Organ

Fe-superoxide dismutase and 2-hydroxy-1,4-benzoquinone reductase preclude the auto-oxidation step in 4-aminophenol metabolism by Burkholderia sp. strain AK-5

Electrochemical Study on DNA Damage Based on the Direct Oxidation of 8‐Hydroxydeoxyguanosine at an Electrochemically Modified Glassy Carbon Electrode

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