Mutagenicity of two species of the genus Alchornea measured by Salmonella microsome assay and micronucleus test

Santos, Fábio V.; Calvo, Tamara Regina; Cólus, I.M.S.; Vilegas, Wagner; Varanda, Eliana Aparecida

doi:10.1590/s0102-695x2010000300015

Cited by 17 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies have demonstrated that natural products can represent serious risks to the DNA integrity of different organisms. 31,32 The methodology employed herein affirms that the extract of P. torta is mutagenic in vivo and in vitro in the absence of metabolic activation. The in vivo test would reflect the metabolism of the active constituents and the metabolization is more complex.…”

Section: Mutagenicity Assayssupporting

confidence: 56%

Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Costa

Rinaldo²,

Varanda³

et al. 2014

Journal of Medicinal Food

Self Cite

View full text Add to dashboard Cite

It is well known that phytotherapy has grown in popularity in recent years. Because a drug cannot be administered without ensuring its effectiveness and safety, the standardization and regulation of phytotherapeutic drugs are required by the global market and governmental authorities. This article describes a simple and reliable high-performance liquid chromatography-diode array detection analysis method for the simultaneous detection of myricetin-3-O-b-Dgalactopyranoside, myricetin-3-O-a-L-arabinopyranoside, and myricetin-3-O-a-L-rhaminopyranoside present in the hydroethanolic extract (ethanol/H2O, 7:3, v/v) of Pouteria torta. The mutagenic activity of the extract was evaluated on Salmonella typhimurium and by an in vivo micronucleus test on the peripheral blood cells of Swiss mice. The linearity, sensitivity, selectivity, repeatability, accuracy, and precision of the assay were evaluated. The analytical curves were linear and exhibited good repeatability (with a deviation of less than 5%) and demonstrated good recovery (within the 83-107% range). The results demonstrate that the hydroethanolic extract exhibited a mutagenic activity in both assays, suggesting caution in the use of this plant in folk medicine.

show abstract

Section: Mutagenicity Assayssupporting

confidence: 56%

Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Costa

Rinaldo²,

Varanda³

et al. 2014

Journal of Medicinal Food

Self Cite

View full text Add to dashboard Cite

show abstract

“…The mutagenic index (MI) was also calculated for each dose, as the average number of revertants per plate divided by average number of revertants per plate of the negative (solvent) control. A sample was considered positive when MI≥2 for at least one of the tested doses and if the response was dose dependent (Varella et al, 2004;Santos et al, 2010).…”

Section: Ames Mutagenicity Assaymentioning

confidence: 99%

Assessment of estrogenic, mutagenic and antimutagenic activity of nemorosone

Camargo¹,

Varela²,

Oliveira³

et al. 2011

Rev. bras. farmacogn.

Self Cite

View full text Add to dashboard Cite

Currently, a wide range of research involving natural products is focused on the discovery of new drugs in many different therapeutic areas. A great number of the synthetic compounds on the market were derived from natural products, especially plants. Nemorosone is the major constituent of the floral resin of Clusia rosea Jacq., Clusiaceae, and in Cuban propolis. In vitro studies have shown cytotoxic activity in this substance against various tumor cell lines, including those resistant to various cytotoxic drugs, whereas it has low cytotoxicity to non-tumoral cells. Therefore, in order to characterize the biological activity of nemorosone, a substance with potential antitumor activity, and in view of preclinical testing of the toxicity of drug candidate compounds, the main aim of this study was to determine the mutagenic and antimutagenic activity of nemorosone by the Ames test, using the strains TA97a, TA98, TA100 and TA102 of Salmonella typhimurium. Secondly, to characterize the estrogenic activity in an experimental recombinant yeast model (Recombinant Yeast Assay) mutagenic activity was observed at in any of the concentrations in any of the test strains. To evaluate the antimutagenic potential, direct and indirect mutagenic agents were used: 4 nitro-o-phenylenediamine (NPD), mitomycin C (MMC) and aflatoxin B1 (AFL). Nemorosone showed moderate antimutagenic activity (inhibition level 31%), in strain TA100 in the presence of AFL, and strong antimutagenic activity in TA102 against MMC (inhibition level 53%). Estrogenic activity was observed, with an EEq of 0.41±0.16 nM at various tested concentrations.

show abstract

“…Several plants have been used as folk medicine for the treatment of several diseases (Melo-Reis et al, 2011), some of which, such as Solanum validinervium (Suarez et al, 2006), Morinda citrifolia (Brito et al, 2009) and Annona squamosa (Nandhakumar & Indumathi, 2012), showed highly interesting characteristics, while others, such as Bauhinia monandra (Macêdo et al, 2008), Alchornea grandulosa, A. castaneaefolia (Santos et al, 2010) and Cochlospermum regium (Castro et al, 2004;Andrade et al, 2008), proved to be genotoxic.…”

Section: Introductionmentioning

confidence: 99%

Morphoanatomic characterization of the stem and the leaf of Tabernaemontana catharinensis A.DC (Apocynaceae) and antimutagenic activity of its leaves

Guidoti

Rocha

Mourão

2015

Rev. bras. plantas med.

View full text Add to dashboard Cite

Tabernaemontana catharinensis A. DC (Apocynaceae) is used as a medicinal plant by the population. In order to contribute to the safe use of the plant as herbal medicine, this study aimed to morphoanatomically characterize the aereal vegetative organs of T. catharinensis and to evaluate the leaves' mutagenic and antimutagenic activities. Histological blades of leaves and stem of T. catharinensis were performed; the methionine system (methG1) and Aspergillusnidulans conidia germination analysis were employed for mutagenic and antimutagenic evaluation. The morphoanatomic analysis did not show trichomes in the stem, petiole and leaf. Besides, it was observed both the presence of bi-collateral bundles -except in the foliar apex where the bundles were from the collateral type -as well as anamphistomatic leaf with paracyte stomata and sub-epidermal layer in the region of the leaf edges. The mutagenicity/ antimutagenicity trial indicated a significant decrease of mutation frequency in comparison with the control group and showed that theT. catharinensis had antimutagenic activity within the type, time and form of treatment. Since the germination test showed that the conidia germination was accelerated from the bud phase, activities at the cell cycle level and polarized growth proved to be possible. The morphoanatomic analysis of the leaf and stem associated with the mutagenic and antimutagenic analyses contributes to the safe use of the plant by humans and also for the quality control of a possible phytotherapeutic drug.Keywords: Aspergillus nidulans, genotoxicity, medicinal herbs, methionine system, morphoanatomy.RESUMO: Caracterização morfoanatômica do caule e da folha de Tabernaemontana catharinensis A.DC (Apocynaceae) e atividade antimutagênica de suas folhas Tabernaemontana catharinensis A. DC (Apocynaceae) é utilizada como planta medicinal pela população. A fim de contribuir para o uso seguro da planta como medicinal, este trabalho teve como objetivo caracterizar morfoanatomicamente os órgãos vegetativos aéreos de T. catharinensis e avaliar a atividade mutagênica e antimutagênica de suas folhas. Foram realizados cortes histológicos da folha e do caule de T. catharinensis e, para a avaliação mutagênica e antimutagênica, foi utilizado o sistema metionina (methG1) e análise da germinação de conídios em Aspergillus nidulans. A análise morfoanatômica evidenciou a ausência de tricomas no caule, pecíolo e folha; presença de feixes bicolaterais, com exceção no ápice foliar cujos feixes são do tipo colateral; folha anfiestomática com estômatos paracíticos e camada subepidérmica na região do bordo foliar. O ensaio de mutagenicidade/antimutagenicidade mostrou uma diminuição significativa da frequência de mutação em relação ao controle, indicando que nesse tipo, tempo e forma de tratamento, T. catharinensis apresentou atividade antimutagênica. O ensaio de germinação evidenciou que houve aceleração da germinação dos conídios, a partir da fase de botão, indicando uma possível atuação em nível de ativação de ciclo celu...

show abstract

Mutagenicity of two species of the genus Alchornea measured by Salmonella microsome assay and micronucleus test

Cited by 17 publications

References 32 publications

Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

Assessment of estrogenic, mutagenic and antimutagenic activity of nemorosone

Morphoanatomic characterization of the stem and the leaf of Tabernaemontana catharinensis A.DC (Apocynaceae) and antimutagenic activity of its leaves

Contact Info

Product

Resources

About