Mutans Streptococci and Lactobacilli in Breast-Fed Children with Rampant Caries

Matee, Mecky; Mikx, F. H. M.; Maselle, Samuel Y; Helderman, W.H. van Palenstein

doi:10.1159/000261440

Cited by 95 publications

(95 citation statements)

References 21 publications

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“…[22] and Aas et al [23], demonstrated that caries can occur in the absence of S. mutans. The results of these studies had similar results in which mutans was associated with both caries active and caries free children, contradicting its role in the initiation and progression of caries [24][25][26] In contrast, Carmona et al [9], found that the frequency of S. mutans was 76% in subjects with caries and 24% in subjects without caries showing a statistically significant difference. Ge et al [27], demonstrated that ECC is associated not only with increased levels of S. mutans but also due to elevated levels total streptococci in the mouth.…”

Section: Discussionmentioning

confidence: 92%

“…S. mutans is a dominating species, widespread in populations with low caries prevalence, indicating that the ecological determinants of these bacteria are not necessarily associated with a caries promoting lifestyle [28]. Matee et al [24] and Sullivan et al [25], observed that the level of oral streptococci in the saliva of children cannot predict future caries. The importance of S. mutans in caries etiology has been well documented, but the growing recognition that the cariogenic potential may be determined by complex interactions in dental plaque biofilm rather than solely the virulence properties of a single organism [29] hence, there was no clear relationship between caries experience and the presence of S. mutans.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Distribution of S. mutans and S. sorbinus in Caries Active and Caries Free Children by PCR Approach

Soni¹,

Vasavada²

2015

Int J Oral Craniofac Sci

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Background: Streptococcus mutans (S. mutans) and Streptococcus sorbinus (S. sorbinus) have been considered to be the most important micro-organisms associated with dental caries. Therefore, purpose of this study is to detect and correlate the presence of S. mutans and S. sobrinus in the dental plaque of caries free and caries active children, by using Polymerase chain reaction (PCR) method. Materials and Methods:Twenty patients aged between ages 4-8 years were included in the study. The subjects were divided in two groups: Group A consisting of ten children with early childhood caries and Group B consisting of ten caries-free children. Dental examinations were performed using a plane dental mirror and explorer. Plaque sample was collected from the cervical margin of the teeth by using explorer. PCR testing was performed for S. mutans and S. sorbinus. The data was analyzed by using SPSS software. The caries score data was analyzed among groups by applying Fischer's exact test. The P value <0.05 was considered as significant.Results: S. mutans was present in all patients, whether they were caries active or caries free. There was no statistically significant difference in the presence of S. sorbinus in caries active patients as compared to caries free patients. It was also noticed that S. sorbinus was found more in male patients as compared to females, the difference being statistically non-significant. Conclusion:Children harbouring both S. mutans and S. sorbinus had a higher incidence of dental caries as compared to the presence of either organism alone.Among the oral bacteria, Mutans streptococci have been implicated as a major cariogenic bacteria. The degree of colonization of these organisms correlates with the prevalence of dental caries in children and experimental animals.Mutans streptococci is divided into seven species: Streptococcus mutans, Streptococcus sobrinus, Streptococcus downei, Streptococcus rattus, Streptococcus cricetus, Streptococcus ferus, and Streptococcus macacae. Among these S. mutans and S. sobrinus are strongly associated with human dental caries [6]. Epidemiological studies have reported that S. mutans is more prevalent than S. sorbinus in the oral cavity, but have also shown that the prevalence of S. sorbinus is more closely associated with a high caries experience [7].Molecular methods for bacterial identification and enumeration now make it more precise to study the microbiota associated with caries. DNA sequence-based assays can be used to identify closely related species that are difficult to differentiate by traditional, culturebased approaches [8]. Various methods have been used for detection, including biochemical tests, immunological tests, DNA probes, and Polymerase Chain Reaction (PCR). Among these, the PCR method is currently being applied to the detection of putative pathogens and the identification of human cariogenic bacteria because it is rapid, sensitive, and simple.The purpose of this study is to detect and correlate the presence of S. mutans and S. sobrinus in...

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Distribution of S. mutans and S. sorbinus in Caries Active and Caries Free Children by PCR Approach

Soni¹,

Vasavada²

2015

Int J Oral Craniofac Sci

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“…Previous studies reported that ad libitum breast-feeding during sleep resulted in higher MS counts in dental plaque and more dental caries among young children 9,13) . Other factors associated with this Dental Caries and Prolonged Breast-feeding high caries incidence may be the cariogenic potential of human milk and the tendency to eat other more cariogenic food 6) .…”

Section: Discussionmentioning

confidence: 95%

Longitudinal Study of Prolonged Breast- or Bottle-feeding on Dental Caries in Japanese Children

Yonezu

Ushida

Yakushiji

2006

Bull. Tokyo Dent. Coll.

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The aim of this investigation was to study the effects of prolonged breast-or bottlefeeding on dental caries in Japanese infants. This longitudinal study was conducted by means of a questionnaire and clinical examination of 592 children at 18 months, 2 years and 3 years of age. The children were divided into three groups: 1) children still being breast-fed at 18 months of age (n‫;)24ס‬ 2) children still being bottle-fed at 18 months of age (n‫;)54ס‬ and 3) children weaned off of breast-or bottle-feeding and with no nonnutritive-sucking habits at 18 months of age (n‫.)502ס‬ Results showed that breast-feeding at 18 months of age produced many significant differences to the control children, including a higher prevalence of caries and higher number of dft. However, no significant differences were observed between bottle-fed and control children. In conclusion, our results suggest that prolonged breast-feeding at an early age before primary dentition has fully erupted is a risk factor for dental caries. Therefore, breast-fed children need to be monitored more closely, and aggressive methods of preventive care need to be instituted. It is also important to identify factors related to dental caries among breastfed children as soon as possible, and develop effective preventive programs.

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“…[10,33] However, some recent studies indicate that the relationship between S. mutans, and caries is not absolute high proportions of S. mutans may persist on tooth surfaces without lesion development, and caries can develop in the complete absence of S. mutans. [34,35] Veillonella are an acidogenic Firmicutes with 5.8% of the total population. In this study, 5 patients showed complete absence of this genus in the deep layer.…”

Section: Discussionmentioning

confidence: 99%

Cariogenic microflora and pH in superficial and deep layers of occlusal carious lesions - A metagenomic analysis

S¹,

Sathyanarayanan²,

Usha³

et al. 2017

JCRI

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Background: Streptococcus mutans and Lactobacilli were the primary microorganisms that cause dental caries. However, current molecular microbiology advancements have suggested the possible roles of other microorganisms in causation of carious lesions. Aims and Objectives:The aim is to explore the complete bacterial profile and pH in superficial and deep layer of carious dentinal lesion in reversible pulpitis patient. Materials and Methods: A total of 12 patients with occlusal cavitated lesion were chosen for this study. The carious dentinal sample was collected. The samples were subjected to DNA extraction quantification with 16S rRNA amplification and pH measurement by suspending the carious sample into 0.9% of NaCl solution. Results:The results showed higher number of Actinobacteria followed by Firmicutes, Fusobacteria, Bacteroidetes, and Spirochaetes. The superficial layer was found to be acidic pH. Conclusion:There are more bacteria in the superficial carious layer than in the deep layer, with a fold difference of 2.8%.

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Mutans Streptococci and Lactobacilli in Breast-Fed Children with Rampant Caries

Cited by 95 publications

References 21 publications

Distribution of S. mutans and S. sorbinus in Caries Active and Caries Free Children by PCR Approach

Distribution of S. mutans and S. sorbinus in Caries Active and Caries Free Children by PCR Approach

Longitudinal Study of Prolonged Breast- or Bottle-feeding on Dental Caries in Japanese Children

Cariogenic microflora and pH in superficial and deep layers of occlusal carious lesions - A metagenomic analysis

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