Mutant prevention concentrations of daptomycin for Enterococcus faecium clinical isolates

Sinel, Clara; Jaussaud, Clara; Auzou, Michel; Giard, Jean‐Christophe; Cattoir, Vincent

doi:10.1016/j.ijantimicag.2016.07.006

Cited by 7 publications

(2 citation statements)

References 20 publications

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“…A modified version of the protocol described by Sinel et al [ 59 ] was used to determine the MPC of nisin regarding the 23 S. aureus DFI isolates under study.…”

Section: Methodsmentioning

confidence: 99%

Nisin Mutant Prevention Concentration and the Role of Subinhibitory Concentrations on Resistance Development by Diabetic Foot Staphylococci

et al. 2022

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The most prevalent microorganism in diabetic foot infections (DFI) is Staphylococcus aureus, an important multidrug-resistant pathogen. The antimicrobial peptide nisin is a promising compound for DFI treatment, being effective against S. aureus. However, to avoid the selection of resistant mutants, correct drug therapeutic doses must be established, being also important to understand if nisin subinhibitory concentrations (subMIC) can potentiate resistant genes transfer between clinical isolates or mutations in genes associated with nisin resistance. The mutant selection window (MSW) of nisin was determined for 23 DFI S. aureus isolates; a protocol aiming to prompt vanA horizontal transfer between enterococci to clinical S. aureus was performed; and nisin subMIC effect on resistance evolution was assessed through whole-genome sequencing (WGS) applied to isolates subjected to a MEGA-plate assay. MSW ranged from 5–360 μg/mL for two isolates, from 5–540 μg/mL for three isolates, and from 5–720 μg/mL for one isolate. In the presence of nisin subMIC values, no transconjugants were obtained, indicating that nisin does not seem to promote vanA transfer. Finally, WGS analysis showed that incubation in the presence of nisin subMIC did not promote the occurrence of significant mutations in genes related to nisin resistance, supporting nisin application to DFI treatment.

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“…A modified version of the protocol described by Sinel et al [ 59 ] was used to determine the MPC of nisin regarding the 23 S. aureus DFI isolates under study.…”

Section: Methodsmentioning

confidence: 99%

Nisin Mutant Prevention Concentration and the Role of Subinhibitory Concentrations on Resistance Development by Diabetic Foot Staphylococci

et al. 2022

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“…Thus, DiPippo and colleagues found that exposure to daptomycin within the prior 90 days was associated with a significant risk that subsequent E. faecium bacteremia will be due to a daptomycin non-susceptible (DNS) strain 104 . Furthermore, in vitro studies found that, with commonly used doses of <10 mg/kg/day, free drug Cmax values usually fall within the mutant selection window 105 . In addition, many isolates with MICs of 3-4 mcg/mL (isolates with MIC >4 mcg/mL are non-susceptible) carry mutations, especially in liaFSr , that are associated with loss of daptomycin bactericidal activity 106 , and exposure of these strains to daptomycin concentrations consistent with administration of ≤10 mg/kg/day allowed regrowth 107 .…”

Section: Treatmentmentioning

confidence: 99%

Vancomycin-resistant enterococcus infection in the hematopoietic stem cell transplant recipient: an overview of epidemiology, management, and prevention

Benamu¹,

Deresinski

2018

F1000Res

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Vancomycin-resistant enterococcus (VRE) is now one of the leading causes of nosocomial infections in the United States. Hematopoietic stem cell transplantation (HSCT) recipients are at increased risk of VRE colonization and infection. VRE has emerged as a major cause of bacteremia in this population, raising important clinical questions regarding the role and impact of VRE colonization and infection in HSCT outcomes as well as the optimal means of prevention and treatment. We review here the published literature and scientific advances addressing these thorny issues and provide a rational framework for their approach.

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Inhaled Antimicrobials for Ventilator-Associated Pneumonia: Practical Aspects

Poulakou

Matthaiou

Nicolau

et al. 2017

Drugs

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Mutant prevention concentrations of daptomycin for Enterococcus faecium clinical isolates

Cited by 7 publications

References 20 publications

Nisin Mutant Prevention Concentration and the Role of Subinhibitory Concentrations on Resistance Development by Diabetic Foot Staphylococci

Nisin Mutant Prevention Concentration and the Role of Subinhibitory Concentrations on Resistance Development by Diabetic Foot Staphylococci

Vancomycin-resistant enterococcus infection in the hematopoietic stem cell transplant recipient: an overview of epidemiology, management, and prevention

Inhaled Antimicrobials for Ventilator-Associated Pneumonia: Practical Aspects

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