Mutants of Anabaena sp. PCC 7120 lacking alr1690 and α-furA antisense RNA show a pleiotropic phenotype and altered photosynthetic machinery

Hernández, José Ángel; Alonso, Idoia; Pellicer, Silvia; Peleato, M. Luisa; Cases, R.; Strasser, Reto J.; Barja, François; Fillat, Marı́a F.

doi:10.1016/j.jplph.2009.10.009

Cited by 33 publications

(23 citation statements)

References 33 publications

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“…Another intriguing example is an aTSS within furA (sll0567) encoding the ferric uptake regulator. Expression of the furA ortholog in the cyanobacterium Anabaena PCC7120 is finetuned by antisense transcription (28), with important consequences for iron homeostasis (29).…”

Section: Resultsmentioning

confidence: 99%

An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803

Mitschke

Georg

Scholz

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

350

459

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There has been an increasing interest in cyanobacteria because these photosynthetic organisms convert solar energy into biomass and because of their potential for the production of biofuels. However, the exploitation of cyanobacteria for bioengineering requires knowledge of their transcriptional organization. Using differential RNA sequencing, we have established a genome-wide map of 3,527 transcriptional start sites (TSS) of the model organism Synechocystis sp. PCC6803. One-third of all TSS were located upstream of an annotated gene; another third were on the reverse complementary strand of 866 genes, suggesting massive antisense transcription. Orphan TSS located in intergenic regions led us to predict 314 noncoding RNAs (ncRNAs). Complementary microarray-based RNA profiling verified a high number of noncoding transcripts and identified strong ncRNA regulations. Thus, ∼64% of all TSS give rise to antisense or ncRNAs in a genome that is to 87% protein coding. Our data enhance the information on promoters by a factor of 40, suggest the existence of additional small peptide-encoding mRNAs, and provide corrected 5′ annotations for many genes of this cyanobacterium. The global TSS map will facilitate the use of Synechocystis sp. PCC6803 as a model organism for further research on photosynthesis and energy research.gene expression regulation | promoter prediction | RNA polymerase

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Section: Resultsmentioning

confidence: 99%

An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803

Mitschke

Georg

Scholz

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

350

459

View full text Add to dashboard Cite

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“…In Anabaena PCC7120, the alr1690 gene has an extremely long 3Ј UTR that overlaps the full length of the gene for the ferric uptake regulator furA. Indeed, in an ⌬alr1690 strain, increased levels of the Fur protein and an iron deficiency phenotype were observed (35,36). For other asRNAs, the longer part of the transcript lies antisense to a gene, but the nonoverlapping part may contain a small open reading frame, as in the case of as_slr0882 in Synechocystis PCC6803 (27).…”

Section: Various Types Of Antisense Transcripts In Bacteriamentioning

confidence: 99%

cis-Antisense RNA, Another Level of Gene Regulation in Bacteria

Georg

Hess

2011

Microbiol Mol Biol Rev

364

348

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SUMMARY A substantial amount of antisense transcription is a hallmark of gene expression in eukaryotes. However, antisense transcription was first demonstrated in bacteria almost 50 years ago. The transcriptomes of bacteria as different as Helicobacter pylori , Bacillus subtilis , Escherichia coli , Synechocystis sp. strain PCC6803, Mycoplasma pneumoniae , Sinorhizobium meliloti , Geobacter sulfurreducens , Vibrio cholerae , Chlamydia trachomatis , Pseudomonas syringae , and Staphylococcus aureus have now been reported to contain antisense RNA (asRNA) transcripts for a high percentage of genes. Bacterial asRNAs share functional similarities with trans -acting regulatory RNAs, but in addition, they use their own distinct mechanisms. Among their confirmed functional roles are transcription termination, codegradation, control of translation, transcriptional interference, and enhanced stability of their respective target transcripts. Here, we review recent publications indicating that asRNAs occur as frequently in simple unicellular bacteria as they do in higher organisms, and we provide a comprehensive overview of the experimentally confirmed characteristics of asRNA actions and intimately linked quantitative aspects. Emerging functional data suggest that asRNAs in bacteria mediate a plethora of effects and are involved in far more processes than were previously anticipated. Thus, the functional impact of asRNAs should be considered when developing new strategies against pathogenic bacteria and when optimizing bacterial strains for biotechnology.

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“…It has been hypothesized that α-furA RNA masks the ribosome binding site in the furA mRNA, and thus interferes with furA expression, which in turn alters the level of proteins belonging to the furA regulon. Mutants impaired in α-furA-alr1690 dicistron resulted in an increased expression of furA and exhibited an iron-deficient phenotype (Hernandez et al 2010). In M. aeruginosa PCC 7806, implication of α-fur RNA has been mainly observed as a consequence of oxidative stress and change in light conditions ( Martin-Luna et al 2011).…”

Section: Regulation Of Fur In Cyanobacteriamentioning

confidence: 99%

Ferric Uptake Regulator (FUR) protein: properties and implications in cyanobacteria

et al. 2015

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The Ferric uptake regulator (Fur) protein is a global iron regulator found in most prokaryotes. Although the Fur protein is involved in a variety of metabolic pathways, it is specifically known for the regulation of several iron responsive genes. It binds to the highly conserved sequences located in the upstream promoter region known as iron boxes, using ferrous ion as a co-repressor. Apart from that, the Fur protein is also directly/indirectly involved in a variety of other crucial physiological pathways. Hence, understanding the mechanism of action and the mechanistic pathways of iron regulation by Fur is necessary and important. The basic understanding of the functioning and properties of Fur protein along with its role, interaction and regulation at various levels in cyanobacteria has been discussed in detail.

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Mutants of Anabaena sp. PCC 7120 lacking alr1690 and α-furA antisense RNA show a pleiotropic phenotype and altered photosynthetic machinery

Cited by 33 publications

References 33 publications

An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803

An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803

cis-Antisense RNA, Another Level of Gene Regulation in Bacteria

Ferric Uptake Regulator (FUR) protein: properties and implications in cyanobacteria

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