Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase

Andro, Thierry; Chambost, Jean-Pierre; Kotoujansky, Alain; Cattanéo, J.; Bertheau, Yves; Barras, Frédéric; Gijsegem, Frédérique Van; Coleno, Alain

doi:10.1128/jb.160.3.1199-1203.1984

Cited by 236 publications

(79 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pel activity was detected by the cup-plate technique (Andro et al, 1984). When necessary, Pel activity was assessed in CSs as reported previously (Franza et al, 1999).…”

Section: Determination Of Pel Activitymentioning

confidence: 99%

Dickeya dadantii pectic enzymes necessary for virulence are also responsible for activation of the Arabidopsis thaliana innate immune system

Expert

Patrit

Shevchik

et al. 2017

Molecular Plant Pathology

View full text Add to dashboard Cite

Soft-rot diseases of plants attributed to Dickeya dadantii result from lysis of the plant cell wall caused by pectic enzymes released by the bacterial cell by a type II secretion system (T2SS). Arabidopsis thaliana can express several lines of defence against this bacterium. We employed bacterial mutants with defective envelope structures or secreted proteins to examine early plant defence reactions. We focused on the production of AtrbohD-dependent reactive oxygen species (ROS), callose deposition and cell death as indicators of these reactions. We observed a significant reduction in ROS and callose formation with a bacterial mutant in which genes encoding five pectate lyases (Pels) were disrupted. Treatment of plant leaves with bacterial culture filtrates containing Pels resulted in ROS and callose production, and both reactions were dependent on a functional AtrbohD gene. ROS and callose were produced in response to treatment with a cellular fraction of a T2SS-negative mutant grown in a Pels-inducing medium. Finally, ROS and callose were produced in leaves treated with purified Pels that had also been shown to induce the expression of jasmonic acid-dependent defence genes. Pel catalytic activity is required for the induction of ROS accumulation. In contrast, cell death observed in leaves infected with the wild-type strain appeared to be independent of a functional AtrbohD gene. It was also independent of the bacterial production of pectic enzymes and the type III secretion system (T3SS). In conclusion, the work presented here shows that D. dadantii is recognized by the A. thaliana innate immune system through the action of pectic enzymes secreted by bacteria at the site of infection. This recognition leads to AtrbohD-dependent ROS and callose accumulation, but not cell death.

show abstract

“…Pel activity was detected by the cup-plate technique (Andro et al, 1984). When necessary, Pel activity was assessed in CSs as reported previously (Franza et al, 1999).…”

Section: Determination Of Pel Activitymentioning

confidence: 99%

Dickeya dadantii pectic enzymes necessary for virulence are also responsible for activation of the Arabidopsis thaliana innate immune system

Expert

Patrit

Shevchik

et al. 2017

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…duction by the strain was determined as described earlier (Andro et al 1984). Briefly, M9 medium agar (Miller 1974) amended with cellulose (10 g ml )1 ), in which a clear halo after 3 days of incubation of the colonies at 28°C was considered as positive for cellulase production.…”

Section: Detection and Estimation Of Plant-growth-promoting Phytohormmentioning

confidence: 99%

Isolation and characterization of a novel banana rhizosphere bacterium as fungal antagonist and microbial adjuvant in micropropagation of banana

et al. 2006

View full text Add to dashboard Cite

Aim: Isolation and characterization of a bacterial isolate (strain FP10) from banana rhizosphere with innate potential as fungal antagonist and microbial adjuvant in micropropagation of banana. Methods and Results: Bacterium FP10 was isolated from the banana rhizosphere and identified as Pseudomonas aeruginosa based on phenotypic, biochemical traits and sequence homology of partial 622‐bp fragment of 16S ribosomal DNA (rDNA) amplicon, with the ribosomal database sequences. Strain FP10 displayed antibiosis towards fungi causing wilt and root necrosis diseases of banana. Production of plant growth hormone, indole‐3‐acetic acid (IAA), siderophores and phosphate‐solubilizing enzyme in FP10 was determined. Strain FP10 tested negative for hydrogen cyanide, cellulase and pectinase, the deleterious traits for plant growth. Screening of antibiotic genes was carried out by polymerase chain reaction using gene‐specific primers. Amplification of a 745‐bp DNA fragment confirmed the presence of phlD, which is a key gene involved in the biosynthesis of 2,4‐diacetylphloroglucinol (DAPG) in FP10. The antibiotic produced by FP10 was confirmed as DAPG using thin layer chromatography, high performance liquid chromatography and Fourier transform infrared and tested for fungal antibiosis towards banana pathogens. Procedures for encapsulation of banana shoot tips with FP10 are described. Conclusions: Strain FP10 exhibited broad‐spectrum antibiosis towards banana fungi causing wilt and root necrosis. DAPG by FP10 induced bulb formation and lysis of fungal mycelia. Encapsulation of banana shoot tips with FP10 induced higher frequency of germination (plantlet development) than nontreated controls on Murashige and Skoog basal medium. Treatment of banana plants with FP10 enhanced plant height and reduced the vascular discolouration as a result of Fusarium oxysporum f. sp. cubense FOC. Significance and Impact of the Study: Because of the innate potential of fungal antibiosis by DAPG antibiotic and production of siderophore, plant‐growth‐promoting IAA and phosphatase, the strain FP10 can be used as biofertilizer as well as a biocontrol agent.

show abstract

“…To examine the role of the PccS1 eda gene in pectin utilization, the exoenzyme activity of the wild-type and mutant strains was assayed. Briefly, 10 lL of an overnight culture (normalized to 10 4 cells/mL in 10 mM MgSO 4 ) were spotted onto pectinase plates as described in Andro et al (1984) and incubated at 278C for 72 h, followed by flooding with 7.5% (w/v) copper acetate for 1-2 h. Colonies positive for Pel activity produce creamy double haloes on a translucent light blue background. Three repetitions were performed for each assay.…”

Section: Exo-enzyme Activity Assaysmentioning

confidence: 99%

Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence

Wang

Zhou

et al. 2016

Molecular Plant Pathology

View full text Add to dashboard Cite

The identification of phytopathogen proteins that are differentially expressed during the course of the establishment of an infection is important to better understand the infection process. In vitro approaches, using plant extracts added to culture medium, have been used to identify such proteins, but the biological relevance of these findings for in planta infection are often uncertain until confirmed by in vivo studies. Here, we compared the proteins of Pectobacterium carotovorum ssp. carotovorum strain PccS1 differentially expressed in Luria-Bertani medium supplemented with extracts of the ornamental plant Zantedeschia elliotiana cultivar 'Black Magic' (in vitro) and in plant tissues (in vivo) by two-dimensional electrophoresis coupled with mass spectrometry. A total of 53 differentially expressed proteins (>1.5-fold) were identified (up-regulated or down-regulated in vitro, in vivo or both). Proteins that exhibited increased expression in vivo but not in vitro, or in both conditions, were identified, and deletions were made in a number of genes encoding these proteins, four of which (clpP, mreB, flgK and eda) led to a loss of virulence on Z. elliotiana, although clpP and mreB were later also shown to be reduced in growth in rich and minimal media. Although clpP, flgK and mreB have previously been reported as playing a role in virulence in plants, this is the first report of such a role for eda, which encodes 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase, a key enzyme in Entner-Doudoroff metabolism. The results highlight the value of undertaking in vivo as well as in vitro approaches for the identification of new bacterial virulence factors.

show abstract

Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase

Cited by 236 publications

References 17 publications

Dickeya dadantii pectic enzymes necessary for virulence are also responsible for activation of the Arabidopsis thaliana innate immune system

Dickeya dadantii pectic enzymes necessary for virulence are also responsible for activation of the Arabidopsis thaliana innate immune system

Isolation and characterization of a novel banana rhizosphere bacterium as fungal antagonist and microbial adjuvant in micropropagation of banana

Characterization of Pectobacterium carotovorum proteins differentially expressed during infection of Zantedeschia elliotiana in vivo and in vitro which are essential for virulence

Contact Info

Product

Resources

About