Mutation Breeding Using Gamma Rays to Increase Seed Germination in Rosa Hybrid

Abstract

Breeders are always looking for new and novel varieties to be competitive in the flower market. Low seed germination rate is still a major problem encountered in traditional rose breeding programs. Mutagenic agents, such as ionizing radiation, may be used to enhance seed vigor and increase the productivity, improving sprouting and emergence of buds that are carried out through seed coating. The effects of ionizing radiation on seed vigor are in most cases genotype-dependent, thus irradiation treatments need to be optimised for each different cultivar. In the present study, gamma-rays (0, 50, 100 and 200 Gy) were applied on hybrid tea rose seeds in order to set up a radiation protocol for increasing seed germination. Seeds coming from six different crossings of Rosa hybrida commercial cultivars were used in order to gain information on radiotolerance and germination ability.

An Effort of Mutation Breeding by Oryzalin and Gamma Rays on Wild Raspberry (Rubus sp.) in Cibodas Botanical Garden

“…It is slightly different with certain studies performed on seeds of several species such as Withania samnifera (Bharathi et al, 2013), Citrus jambhiri (Sharma et al, 2013), Cajanus cajan (Ariraman et al, 2014) that a gradual decrease in seed germination from lower doses to higher doses of gamma rays were observed. In the other hand, Giovani et al (2015) reported that the effect of gamma rays at 50, 100 and 200 Gy were able to increase seed germination of Rosa hybrida. Their results are in line with our experiment, which was the seed germination of R. lineatus increase in 50,100,150,200,250,300,400,450 Gy and R. chrysophyllus in 100 Gy.…”

Radiación gamma de 60Co en características morfológicas y reproductivas de plantas M1 en Coffea arabica L.

“…En los últimos años se ha intensificado la utilización de las mutaciones radioinducidas en el mejoramiento genético en diferentes especies, en ornamentales (Chanchula et al 2015, Giovannini et al 2015, Patil et al 2015 Considerando que el café es una planta autogama, su variabilidad genética es reducida aunado a que presenta autoincompatibilidad genética y poliploidia en especies comerciales, es necesario iniciar programas de mejoramiento genético por mutagénesis radioinducida como un complemento al mejoramiento genético convencional (Villalta-Villalobos y Gatica-Arias 2019). Para iniciar un programa de mejoramiento genético por mutagénesis, se debe determinar la dosis del mutágeno; DL 50 y GR 50 que permita una óptima frecuencia de mutantes en la población objetivo (Lamo et al 2017).…”