Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae)

Jiang, Xiaojing; Qu, Mingjing; Denholm, I.; Fang, Jichao; Jiang, Weihua; Han, Zhaojun

doi:10.1016/j.bbrc.2008.11.046

Cited by 94 publications

(79 citation statements)

References 26 publications

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“…Generally, AChE1 is the major enzyme in insects, which is more abundant than AChE2. The expression of ace1 is higher than ace2 in some insect species 9, 10, 18, 19 , while the opposite is true in others. The AChE2 in B. mori and Apis mellifera is the major enzyme in synaptic transmission 20, 21 .…”

Section: Introductionmentioning

confidence: 85%

Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways

Yang

Stanley

et al. 2017

Sci Rep

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Among its other biological roles, acetylcholinesterase (AChE, EC 3.1.1.7), encoded by two ace in most insects, catalyses the breakdown of acetylcholine, thereby terminating synaptic transmission. ace1 encodes the synaptic enzyme and ace2 has other essential actions in many insect species, such as Chilo suppressalis and Plutella xylostella. The silkworm, Bombyx mori, has been domesticated for more than two thousand years and its aces have no history of pesticide exposure. Here, we investigated the functional differences between two ace genes, BmAce1 and BmAce2, in the silkworm. qPCR analysis indicated that BmAce1 is highly expressed in muscle and BmAce2 is more ubiquitously expressed among tissues and enriched in the head. Both genes were separately suppressed using chemically synthesized siRNAs. The mRNA abundance of the two ace genes was significantly reduced to about 13% – 75% of the control levels after siRNA injection. The AChE activities were decreased to 32% to 85% of control levels. Silencing BmAce2 resulted in about 26% mortality, faster and higher than the 20% in the siBmAce1-treated group. Silencing BmAce1 impacted motor control and development to a greater extent than silencing BmAce2, although both treatment groups suffered motor disability, slowed development and reduced cocoons. Both genes have essential, differing biological significance.

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Section: Introductionmentioning

confidence: 85%

Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways

Yang

Stanley

et al. 2017

Sci Rep

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“…However, in several cases, AChE activity was found unaltered in association with resistance (Zhu and Brindley, 1990), and even in a few cases, an increase in the activity was reported in resistant insects (Plapp and Tripathi, 1978;Zhu and Gao, 1999;Chai et al, 2007;Yang et al, 2008). Additionally, in the last several years, molecular studies have shown that several insect species possess more than one form of AChE (Li and Han, 2002a;Jiang et al, 2008) and this was considered to be another important aspect of AChE involved in insecticide resistance. In the current study, AChE was successfully purified from three field populations of L. entomophila by procainamide-based affinity chromatography and details of their biochemical characteristics are reported here for the first time.…”

Section: Discussionmentioning

confidence: 92%

Comparative studies of acetylcholinesterase purified from three field populations of Liposcelis entomophila (enderlein) (psocoptera: liposcelididae)

Xiao

Dou

et al. 2010

Arch Insect Biochem Physiol

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Acetylcholinesterace (AChE) is known to be the major target for organophophate and carbamate insecticides and biomolecular changes to AChE have been demonstrated to be an important mechanism for insecticide resistance in many insect species. In this study, AChE from three field populations of Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae) was purified by affinity chromatography and subsequently characterized by its Michaelis-Menten kinetics to determine if detectable changes to AChE have occurred. Bioassays revealed that the potential resistance threat of psocids in Sichuan Province (GH) was greater than either Hubei Province (WH) or Chongqing Municipality (BB). Compared to the other two populations, the WH population possessed the highest specific activity of purified AChE. Kinetic analyses indicated that the purified AChE from GH population expressed a significantly lower affinity to the substrate and a higher catalytic activity toward acetylthiocholine iodide (ATChI) (i.e., higher K(m) and V(max) values) than BB and WH populations. In vitro studies of AChE suggest that five inhibitors (aldicarb, eserine, BW284C51, omethoate, and propoxur) all possess strong inhibitory effects with eserine having the strongest inhibitory effect against purified AChE. According to bimolecular rate constants (k(i)), the purified AChE from GH population was least sensitive to all inhibitors except for omethoate. The differences in AChE among the three populations may be partially attributed to the differences in pesticide application and control practices for psocids among the three locations.

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“…Full length cDNA cloning and phylogenetic analysis of the translation products suggest that the gene product of cface1, CfAChE1, belongs to the AChE1 family first identified in aphids (Gao et al, 2002;Li and Han, 2002) and mosquitos (Weill et al, 2002) and present in numerous insect species, but absent in cyclorraphan diptera (Huchard et al, 2006). By contrast the cface2-encoded CfAChE2 belongs to the AChE2 family typical of cyclorraphan diptera (Huchard et al, 2006), but whose members are also present in other insects possessing cface1 genes, including several mosquito species (Weill et al, 2003(Weill et al, , 2004Nabeshima et al, 2004;Alout and Weill 2008), aphids (Li and Han, 2002), cockroaches , several lepidopterans (Cassanelli et al, 2006;Lee et al, 2006;Jiang et al, 2009) or honey bees (Badiou et al, 2007).…”

Section: Discussionmentioning

confidence: 94%

“…In mosquitos (Weill et al, 2003(Weill et al, , 2004Nabeshima et al, 2004;Alout and Weill 2008) and several other insect species (Li and Han 2004;Benting and Nauen, 2004;Cassanelli et al, 2006;Lee et al, 2006;Chen et al, 2007;Lee et al, 2007;Alon et al, 2008;Jiang et al, 2009), AChE target site insensitivity to OP and MC insecticides implicates only the ace1 and not the ace2 locus, while in cyclorraphan diptera, mutations in the ace2 locus were shown to be responsible for OP resistance (Kozaki et al, 2001;Walsh et al, 2001;Vontas et al, 2002).…”

Section: Introductionmentioning

confidence: 98%

Acetylcholinesterases of the cat flea Ctenocephalides felis: identification of two distinct genes and biochemical characterization of recombinant and in vivo enzyme activities

Ilg¹,

Schmalz²,

Werr³

et al. 2010

Insect Biochemistry and Molecular Biology

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Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae)

Cited by 94 publications

References 26 publications

Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways

Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways

Comparative studies of acetylcholinesterase purified from three field populations of Liposcelis entomophila (enderlein) (psocoptera: liposcelididae)

Acetylcholinesterases of the cat flea Ctenocephalides felis: identification of two distinct genes and biochemical characterization of recombinant and in vivo enzyme activities

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