2001
DOI: 10.1074/jbc.m101185200
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Mutation in PMR1, a Ca2+-ATPase in Golgi, Confers Salt Tolerance in Saccharomyces cerevisiae by Inducing Expression of PMR2, an Na+-ATPase in Plasma Membrane

Abstract: Sodium tolerance in yeast is enhanced by continuous activation of calcineurin, a Ca2؉ /calmodulin-dependent protein phosphatase that is required for modulation of the Na ؉ efflux mechanism. We isolated several salt-tolerant mutations with the treatment of ethylmethane sulfonate under high salt stress. One of the mutations was mapped in the PMR1 gene. Pmr1p, the P-type Ca 2؉ -ATPase in the Golgi apparatus, regulates a cytosolic Ca 2؉ level in various responses. Cytosolic Ca 2؉ concentration in the pmr1 mutant i… Show more

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Cited by 39 publications
(37 citation statements)
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“…In contrast to the S. cerevisiae PMR1 mutant, which exhibits growth sensitivity to Ca 2ϩ and Mn 2ϩ (1,13,18,20), our data revealed cation tolerance in the ⌬pmrA strain. This discrepancy prompted us to investigate the expression levels of the major genes of the calcineurin pathway, including cnaA, crzA, Triplicate RT-PCR assays were performed using 1ϫ iQ SYBR green master mix with two biological replicates, and expression levels were normalized to those for ␤-tubulin.…”
mentioning
confidence: 51%
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“…In contrast to the S. cerevisiae PMR1 mutant, which exhibits growth sensitivity to Ca 2ϩ and Mn 2ϩ (1,13,18,20), our data revealed cation tolerance in the ⌬pmrA strain. This discrepancy prompted us to investigate the expression levels of the major genes of the calcineurin pathway, including cnaA, crzA, Triplicate RT-PCR assays were performed using 1ϫ iQ SYBR green master mix with two biological replicates, and expression levels were normalized to those for ␤-tubulin.…”
mentioning
confidence: 51%
“…PMR1, encoding the Ca 2ϩ /Mn 2ϩ P-type ATPase, localizes to the Golgi apparatus, where it mediates Ca 2ϩ homeostasis and supplies Mn 2ϩ required for protein glycosylation (7). The S. cerevisiae PMR1 mutant secretes misfolded proteins and exhibits defective growth under low-Ca 2ϩ conditions and in the presence of Mn 2ϩ (1,13,18,20). In Candida albicans, PMR1 deletion results in loss of virulence (2).…”
mentioning
confidence: 99%
“…About 40% of the total response to high pH seems to be mediated by activation of calcineurin (69,84 (21). Similarly, pmr1 mutants, which are deficient in a P-type ATPase required for Ca 2ϩ and Mn 2ϩ transport into the Golgi apparatus, show higher-than-normal ENA1 expression levels (66).…”
mentioning
confidence: 99%
“…The accumulation of sodium or lithium cations in calcineurin-deficient cells could be attributed, at least in part, to a decrease in efflux capacity as a result of impaired induction of ENA1 expression (38,51,60). In contrast, hyperactivation of calcineurin increases ENA1 expression and improves salt tolerance (59,66). Calcineurin activation of ENA1 is mediated by dephosphorylation of Crz1 (57,86).…”
mentioning
confidence: 99%
“…Pmr1 functions as a Ca 2+ pump and maintains the high Ca 2+ concentration of the Golgi apparatus (Baelen et al, 2004). Since Ca 2+ is an important signalling molecule of the cell, it is not unexpected that the PMR1-disrupted mutant displayed pleiotropic changes, such as a Ca 2+ -dependent growth defect (Antebi et al, 1992), secretion of an unprocessed α factor (Harmsen et al, 1993), incomplete outer-chain glycosylation (Rudolph et al, 1989), salt tolerance (Park et al, 2001), cell shape (Cortes et al, 2004), virulence (Bates et al, 2005) and viability (Agaphonov et al, 2007). In the past few years, PMR1 has received more attention due to the recognition that the mutation of human homologue of this gene causes chronic benign pemphigus or Hailey-Hailey disease (HHD; Hu et al, 2000), and PMR1-dirupted Saccharomyces cerevisiae can function as a useful model organism for studying the molecular pathology of HHD (Ton et al, 2002).…”
Section: Introductionmentioning
confidence: 99%