“…Pmr1 functions as a Ca 2+ pump and maintains the high Ca 2+ concentration of the Golgi apparatus (Baelen et al, 2004). Since Ca 2+ is an important signalling molecule of the cell, it is not unexpected that the PMR1-disrupted mutant displayed pleiotropic changes, such as a Ca 2+ -dependent growth defect (Antebi et al, 1992), secretion of an unprocessed α factor (Harmsen et al, 1993), incomplete outer-chain glycosylation (Rudolph et al, 1989), salt tolerance (Park et al, 2001), cell shape (Cortes et al, 2004), virulence (Bates et al, 2005) and viability (Agaphonov et al, 2007). In the past few years, PMR1 has received more attention due to the recognition that the mutation of human homologue of this gene causes chronic benign pemphigus or Hailey-Hailey disease (HHD; Hu et al, 2000), and PMR1-dirupted Saccharomyces cerevisiae can function as a useful model organism for studying the molecular pathology of HHD (Ton et al, 2002).…”