Mutation induced by vibriophage PS166 infection changes biotype and phage type of Vibrio cholerae

Mitra, Srabani

doi:10.1099/00222615-30-2-137

Cited by 9 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With a V. cholerae genomic microarray that displayed more than 93% of the predicted genes of the whole genome sequence of El Tor strain N16961, Dziejman et al (9) showed that only seven genes were absent solely in classical strains but present in other strains, leading them to speculate that classical biotype strains may be derived from a primordial environmental strain that was more El Tor like than previously thought. Mitra et al have previously reported the involvement of bacteriophage PS166 in the acquisition of some classical biotypespecific properties by El Tor strains (22,23). Insertion of lysogenic phage genomes in the bacterial chromosome leading to the activation or inactivation of certain genes or expression of new phage-encoded genes is a natural phenomenon in the origination of genetic diversity.…”

Section: Discussionmentioning

confidence: 99%

New Variants of Vibrio cholerae O1 Biotype El Tor with Attributes of the Classical Biotype from Hospitalized Patients with Acute Diarrhea in Bangladesh

et al. 2002

View full text Add to dashboard Cite

The sixth pandemic of cholera and, presumably, the earlier pandemics were caused by the classical biotype of Vibrio cholerae O1, which was progressively replaced by the El Tor biotype representing the seventh cholera pandemic. Although the classical biotype of V. cholerae O1 is extinct, even in southern Bangladesh, the last of the niches where this biotype prevailed, we have identified new varieties of V. cholerae O1, of the El Tor biotype with attributes of the classical biotype, from hospitalized patients with acute diarrhea in Bangladesh. Twentyfour strains of V. cholerae O1 isolated between 1991 and 1994 from hospitalized patients with acute diarrhea in Matlab, a rural area of Bangladesh, were examined for the phenotypic and genotypic traits that distinguish the two biotypes of V. cholerae O1. Standard reference strains of V. cholerae O1 belonging to the classical and El Tor biotypes were used as controls in all of the tests. The phenotypic traits commonly used to distinguish between the El Tor and classical biotypes, including polymyxin B sensitivity, chicken cell agglutination, type of tcpA and rstR genes, and restriction patterns of conserved rRNA genes (ribotypes), differentiated the 24 strains of toxigenic V. cholerae O1 into three types designated the Matlab types. Although all of the strains belonged to ribotypes that have been previously found among El Tor vibrios, type I strains had more traits of the classical biotype while type II and III strains appeared to be more like the El Tor biotype but had some classical biotype properties. These results suggest that, although the classical and El Tor biotypes have different lineages, there are possible naturally occurring genetic hybrids between the classical and El Tor biotypes that can cause cholera and thus provide new insight into the epidemiology of cholera in Bangladesh. Furthermore, the existence of such novel strains may have implications for the development of a cholera vaccine.New epidemic strains of toxigenic Vibrio cholerae have appeared at least twice in recent human history (10). Strains of the classical biotype, which had probably been responsible for most of the epidemic disease in the 19th century and much of the 20th century, were largely replaced as the predominant cause of epidemic cholera by strains of the El Tor biotype in most of the regions where cholera is endemic, beginning in 1961. However, the classical biotype strains reemerged as a predominant epidemic strain in parts of Bangladesh in 1982 (8, 25) and coexisted with the El Tor strains, causing disease until 1993. A second new epidemic strain, carrying the O139 rather than the O1 antigen, emerged in southern Asia in 1992 (7, 24). The O139 and El Tor O1 strains continue to cause epidemics of cholera, and there are indications that the incidence of cholera due to the O139 serogroup is on the rise in parts of India and Bangladesh.The classical and El Tor biotypes of V. cholerae are closely related in their O-antigen biosynthetic genes (21, 31), although these two biotypes diffe...

show abstract

Section: Discussionmentioning

confidence: 99%

New Variants of Vibrio cholerae O1 Biotype El Tor with Attributes of the Classical Biotype from Hospitalized Patients with Acute Diarrhea in Bangladesh

et al. 2002

View full text Add to dashboard Cite

show abstract

“…The infection of V. eltor MAK757 cells with phage PS166 produced plaques with resistant growth in their centers. Some of these resistant colonies were found to exhibit properties of the classical biotype, indicating phage conversion (Mitra, 1989). This resistant growth could be due to a phage receptor mutation or to a phage-immune strain due to lysogenization with phage PS166.…”

Section: Lysogeny Of Phage Ps166 In V Eltor Mak757 Cellsmentioning

confidence: 99%

“…It was proposed that the pandemic strain of one biotype might arise from other biotypes of cholera Vibrio by transduction with a temperate phage or by mutation (Ogg et al, 1978). Earlier, we identified phage PS166, which could convert V. eltor MAK757, serotype Ogawa strains to the classical biotype (Mitra, 1989). However, the phage was not characterized, and the molecular mechanism behind the reported biotype conversion was not known.…”

Section: Introductionmentioning

confidence: 96%

Conversion of Vibrio eltor MAK757 to Classical Biotype: Role of Phage PS166

Mitra

Ghosh

2000

Virology

View full text Add to dashboard Cite

Temperate phage PS166 infection of Vibrio eltor MAK757 resulted in complete changes in all biotype-specific determinants. About 10% of the PS166 lysogens of MAK757 lost their eltor-specific determinants, namely, the ability to produce soluble hemolysin, cell-associated hemagglutinin for chicken erythrocytes, and resistance to polymyxin B, as well as resistance to Mukherjee's group IV phage and sensitivity to eltor phage e4. These lysogens were found to have acquired the properties of classical strains, most significantly becoming sensitive to group IV phage but resistant to eltor-specific e4. The remainder of these lysogens, however, retained their parental biotype and serotype but acquired auxotrophy for glycine and histidine. The differential behavior of the two types of lysogen was due to the integration of the phage PS166 genome at different locations in the host chromosome. A 800-bp BglII fragment was found to contain the attP site. Phage PS166 has a polyhedral head (95 nm in diameter) and a contractile tail (98 nm in length). The phage chromosome is a linear double-stranded DNA of 110 kb and a G + C content of 58.7%.

show abstract

“…Historically, a major emphasis was placed on phage typing of V. cholerae (Basu and Mukerjee, 1968;Chattopadhyay et al ., 1993) and phage therapy was proposed for treatment of cholera in the early years of cholera research. Subsequent studies showed that temperate bacteriophages play a role in the lytic sensitivity and serotype conversion of Vibrio cholerae (Siddiqui and Bhattacharyya, 1982;Mitra, 1989). Recently, there has been renewed interest in V. cholerae phages because of their role in lateral transfer of genes in the environment and emergence of toxigenic strains.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Vibrio cholerae phage isolated from the coastal water of Peru

Talledo

Rivera

Lipp

et al. 2003

Environmental Microbiology

View full text Add to dashboard Cite

A Vibrio cholerae bacteriophage, family Myoviridae, was isolated from seawater collected from the coastal water of Lima, Peru. Genome size was estimated to be 29 kbp. The temperate phage was specific to V. cholerae and infected 12/13 V. cholerae O1 strains and half of the four non-O1/non-O139 strains tested in this study. Vibrio cholerae O139 strains were resistant to infection and highest infection rates were obtained in low nutrient media amended with NaCl or prepared using seawater as diluent.

show abstract

Mutation induced by vibriophage PS166 infection changes biotype and phage type of Vibrio cholerae

Cited by 9 publications

References 9 publications

New Variants of Vibrio cholerae O1 Biotype El Tor with Attributes of the Classical Biotype from Hospitalized Patients with Acute Diarrhea in Bangladesh

New Variants of Vibrio cholerae O1 Biotype El Tor with Attributes of the Classical Biotype from Hospitalized Patients with Acute Diarrhea in Bangladesh

Conversion of Vibrio eltor MAK757 to Classical Biotype: Role of Phage PS166

Characterization of a Vibrio cholerae phage isolated from the coastal water of Peru

Contact Info

Product

Resources

About