2020
DOI: 10.1101/2020.06.26.171819
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Mutation Maker, An Open Source Oligo Design Platform for Protein Engineering

Abstract: Founded on principles of natural selection, directed evolution techniques harness high-throughput gene diversification methodologies to generate proteins with optimized properties for applications in research, therapeutic and industrial processes. However, design of gene libraries is typically hampered by scale and complexity, necessitating development of advanced automation and optimization tools that can improve efficiency and accuracy. At present, automated library design tools are obsolete, functionally li… Show more

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“…A substantial number of software tools for different parts of the bioinformatics pipeline have been described in the literature. For the DNA library design, Mutation Maker and OneClick let users design oligos according to different specifications and much more (Tang et al 2012;Hiraga et al 2021). For the preprocessing of DNA reads, FLASH and PEAR tools merge pairedend reads, Cutadapt and trimmomatic remove adapter sequences (Zhang et al 2014;Bolger et al 2014) and QUASR provides a framework for the quantification and analysis of short reads (Gaidatzis et al 2015).…”
Section: Introductionmentioning
confidence: 99%
“…A substantial number of software tools for different parts of the bioinformatics pipeline have been described in the literature. For the DNA library design, Mutation Maker and OneClick let users design oligos according to different specifications and much more (Tang et al 2012;Hiraga et al 2021). For the preprocessing of DNA reads, FLASH and PEAR tools merge pairedend reads, Cutadapt and trimmomatic remove adapter sequences (Zhang et al 2014;Bolger et al 2014) and QUASR provides a framework for the quantification and analysis of short reads (Gaidatzis et al 2015).…”
Section: Introductionmentioning
confidence: 99%