Mutation of OsDET1 increases chlorophyll content in rice

Huang, Junli; Qin, Feng; Zang, Guangchao; Kang, Zhenhui; Zou, Hanyan; Hu, Feng; Yue, Cai-li; Li, Xianyong; Wang, Guixue

doi:10.1016/j.plantsci.2013.06.003

Cited by 24 publications

(18 citation statements)

References 37 publications

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“…To investigate the role of OsDET1 in rice development, we constructed the vector P OsDET1 ::GUS and introduced it into cv Nipponbare rice. Consistent with our previous results (Huang et al, 2013), GUS activity was detected in almost all tissues and organs, such as young callus, roots, stems, nodes, internodes, leaf sheaths, leaves, and panicles (Fig. 1A).…”

Section: Stress-induced and Spatial Expression Profiles Of Osdet1 In supporting

confidence: 92%

“…Although DET1 plays an important role in plant development, the roles of OsDET1 in rice remain unclear, except for its role in chlorophyll biosynthesis (Huang et al, 2013). Here, we report that OsDET1 influences the sensitivity of rice plants to ABA.…”

mentioning

confidence: 77%

“…Recombinant plasmids were transformed into rice callus by Agrobacterium tumefaciens (EHA105) medium transformation as described previously (Huang et al, 2013). The plants were grown in a greenhouse under a 12-h-light/12-hdark cycle (300 mmol photons m 22 s 21 ) at 30°C and 60% relative humidity.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

et al. 2016

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DEETIOLATED1 (DET1) plays a critical role in developmental and environmental responses in many plants. To date, the functions of OsDET1 in rice (Oryza sativa) have been largely unknown. OsDET1 is an ortholog of Arabidopsis (Arabidopsis thaliana) DET1. Here, we found that OsDET1 is essential for maintaining normal rice development. The repression of OsDET1 had detrimental effects on plant development, and leaded to contradictory phenotypes related to abscisic acid (ABA) in OsDET1 interference (RNAi) plants. We found that OsDET1 is involved in modulating ABA signaling in rice. OsDET1 RNAi plants exhibited an ABA hypersensitivity phenotype. Using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation assays, we determined that OsDET1 interacts physically with DAMAGED-SPECIFIC DNA-BINDING PROTEIN1 (OsDDB1) and CONSTITUTIVE PHOTOMORPHOGENIC10 (COP10); DET1-and DDB1-ASSOCIATED1 binds to the ABA receptors OsPYL5 and OsDDB1. We found that the degradation of OsPYL5 was delayed in OsDET1 RNAi plants. These findings suggest that OsDET1 deficiency disturbs the COP10-DET1-DDB1 complex, which is responsible for ABA receptor (OsPYL) degradation, eventually leading to ABA sensitivity in rice. Additionally, OsDET1 also modulated ABA biosynthesis, as ABA biosynthesis was inhibited in OsDET1 RNAi plants and promoted in OsDET1-overexpressing transgenic plants. In conclusion, our data suggest that OsDET1 plays an important role in maintaining normal development in rice and mediates the cross talk between ABA biosynthesis and ABA signaling pathways in rice.

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Section: Stress-induced and Spatial Expression Profiles Of Osdet1 In supporting

confidence: 92%

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confidence: 77%

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The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

et al. 2016

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“…However, only 53 leaf-color genes of rice have been cloned (14 of them had alleles). Among them, 14 genes function directly in chlorophyll biosynthesis and catabolism (DCBC) [4]–[11], while 6 genes indirectly take part in those two processes (ICBC) [12]–[15]. Sixteen genes are directly involved in the developmental regulation of chloroplast (DDC) [16]–[21], while another 3 genes are involved indirectly (IDC) [22].…”

Section: Introductionmentioning

confidence: 99%

Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp. indica)

et al. 2014

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Leaf-color is an effective marker to identify the hybridization of rice. Leaf-color related genes function in chloroplast development and the photosynthetic pigment biosynthesis of higher plants. The ygl7 (yellow-green leaf 7) is a mutant with spontaneous yellow-green leaf phenotype across the whole lifespan but with no change to its yield traits. We cloned gene Ygl7 (Os03g59640) which encodes a magnesium-chelatase ChlD protein. Expression of ygl7 turns green-leaves to yellow, whereas RNAi-mediated silence of Ygl7 causes a lethal phenotype of the transgenic plants. This indicates the importance of the gene for rice plant. On the other hand, it corroborates that ygl7 is a non-null mutants. The content of photosynthetic pigment is lower in Ygl7 than the wild type, but its light efficiency was comparatively high. All these results indicated that the mutational YGL7 protein does not cause a complete loss of original function but instead acts as a new protein performing a new function. This new function partially includes its preceding function and possesses an additional feature to promote photosynthesis. Chl1, Ygl98, and Ygl3 are three alleles of the OsChlD gene that have been documented previously. However, mutational sites of OsChlD mutant gene and their encoded protein products were different in the three mutants. The three mutants have suppressed grain output. In our experiment, plant materials of three mutants (ygl7, chl1, and ygl98) all exhibited mutational leaf-color during the whole growth period. This result was somewhat different from previous studies. We used ygl7 as female crossed with chl1 and ygl98, respectively. Both the F1 and F2 generation display yellow-green leaf phenotype with their chlorophyll and carotenoid content falling between the values of their parents. Moreover, we noted an important phenomenon: ygl7-NIL's leaf-color is yellow, not yellowy-green, and this is also true of all back-crossed offspring with ygl7.

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“…**: Significant difference at P < 0.01; *: at P < 0.05. treatment led to the oxidation process of the Chl a methyl group (Chettri et al, 1998) to form Chl b in P. squarrosa and T. barbuloides. Detrimental effects of heavy metals on the content of photosynthetic pigments are based on the inhibition of pigment synthesis (Huang et al, 2013) and/or the oxidative damage to pigments that results in changes of the Chl a/b ratio. These results led us to suggest that both species are very sensitive to Cu, and P. squarrosa was also sensitive to Cr exposure.…”

Section: Oxidative Damagementioning

confidence: 99%

Bioaccumulation and oxidative stress impact of Pb, Ni, Cu, and Cr heavy metals in two bryophyte species, Pleurochaete squarrosa and Timmiella barbuloides

Aydoğan¹,

Erdağ²,

Aktaş³

2017

Turk J Bot

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Mutation of OsDET1 increases chlorophyll content in rice

Cited by 24 publications

References 37 publications

The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp. indica)

Bioaccumulation and oxidative stress impact of Pb, Ni, Cu, and Cr heavy metals in two bryophyte species, Pleurochaete squarrosa and Timmiella barbuloides

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