The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

Abstract

DEETIOLATED1 (DET1) plays a critical role in developmental and environmental responses in many plants. To date, the functions of OsDET1 in rice (Oryza sativa) have been largely unknown. OsDET1 is an ortholog of Arabidopsis (Arabidopsis thaliana) DET1. Here, we found that OsDET1 is essential for maintaining normal rice development. The repression of OsDET1 had detrimental effects on plant development, and leaded to contradictory phenotypes related to abscisic acid (ABA) in OsDET1 interference (RNAi) plants. We found that OsDET1 is involved in modulating ABA signaling in rice. OsDET1 RNAi plants exhibited an ABA hypersensitivity phenotype. Using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation assays, we determined that OsDET1 interacts physically with DAMAGED-SPECIFIC DNA-BINDING PROTEIN1 (OsDDB1) and CONSTITUTIVE PHOTOMORPHOGENIC10 (COP10); DET1-and DDB1-ASSOCIATED1 binds to the ABA receptors OsPYL5 and OsDDB1. We found that the degradation of OsPYL5 was delayed in OsDET1 RNAi plants. These findings suggest that OsDET1 deficiency disturbs the COP10-DET1-DDB1 complex, which is responsible for ABA receptor (OsPYL) degradation, eventually leading to ABA sensitivity in rice. Additionally, OsDET1 also modulated ABA biosynthesis, as ABA biosynthesis was inhibited in OsDET1 RNAi plants and promoted in OsDET1-overexpressing transgenic plants. In conclusion, our data suggest that OsDET1 plays an important role in maintaining normal development in rice and mediates the cross talk between ABA biosynthesis and ABA signaling pathways in rice.

QTL mapping and candidate gene mining of flag leaf size traits in Japonica rice based on linkage mapping and genome-wide association study

“…qFWn7 located in the same interval with OsFLW7 identi ed by Xu et al (2017). Known genes OsBAK1 (Li et al, 2009), SNFL1 (He et al, 2018) and OsDET1 (Zang et al, 2016), were found to be located within or nearby the LD interval of qFWn8, qFWn5-2, qFLAn1-2 in this study. Among these genes, OsBAK1 controlling leaf angle and length by regulating brassinosteroid (BRs), and has been observed signi cantly reduce plant height.…”

Genome-Wide Identification of the PHD-Finger Family Genes and Their Responses to Environmental Stresses in Oryza sativa L.

“…For ABA and Cd treatments, the roots of the three-week-old hydroponic rice seedlings were immersed in Yoshida’s culture solution containing either 50 µM ABA [ 59 , 60 , 61 ] or 50 µM Cd for 24 h [ 62 ]. For cold stress, rice seedlings were exposed to 4 °C for 24 h. For water deficit stress, water irrigation was stopped (for 7 days) until the soil water content was decreased to 40% of that under normal conditions.…”

METHYLTRANSFERASE1 and Ripening Modulate Vivipary during Tomato Fruit Development

“…S11, D-F, and S12, D-F). To test other genes that are directly or indirectly involved in ABA biosynthesis (Zang et al, 2016), we also examined the effect of Phytoene Synthase1 (SlPSY1) and Deetiolated1 (SlDET1) on the induction of VP in Cnr fruits. Silencing of SlPSY1 or SlDET1 intensified brown pigmentation in the outer epidermis of Cnr fruits (Supplemental Figs.…”