Mutational analysis of influenza B virus RNA transcription in vitro

Lee, Yeon Sook; Seong, Baik Lin

doi:10.1128/jvi.70.2.1232-1236.1996

Cited by 21 publications

(21 citation statements)

References 28 publications

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“…As mentioned in the introduction, it has been previously shown that the influenza type A polymerase can recognize the influenza B virus promoter in vivo (Muster et al, 1991) and that the polymerases from both influenza A and B viruses are able to transcribe type A and B model RNAs in vitro (Lee and Seong, 1996). However, although the sequences at the termini of influenza C virus RNA segments share conserved nucleotides with both type A and B influenza viruses, it has not been determined whether they could also serve as a promoter for heterotypic polymerases.…”

Section: Activity Of Homotypic Core Protein Complexes On Heterotypic mentioning

confidence: 99%

“…Much less is known about the cis-and trans-acting elements involved in transcription and replication of the genome of influenza B virus. As for influenza A virus, functional RNPs can be isolated from influenza B virions, and the sequences that serve as promoters for the viral polymerase are located at the termini of the viral RNA segments (Lee and Seong, 1996). The NP and P proteins are the minimum set of influenza B virus proteins required for the expression of a model RNA template.…”

Section: Introductionmentioning

confidence: 99%

“…The NP and P proteins are the minimum set of influenza B virus proteins required for the expression of a model RNA template. Furthermore, using RNPs reconstituted in vitro or in vivo, the polymerases from both type A and B influenza viruses were found to be active in transcribing heterotypic RNA templates (Jambrina et al, 1997;Lee and Seong, 1996;Lee and Seong, 1998;Stevens and Barclay, 1998). Moreover, a chimeric influenza A/B transfectant virus in which the neuraminidase gene of influenza A was flanked by the 5Ј and 3Ј noncoding sequences from the NS segment of influenza B virus was found to be viable, although its replication in vitro and in vivo was impaired (Muster et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of the Ability of the Polymerase Complexes of Influenza Viruses Type A, B and C to Assemble into Functional RNPs that Allow Expression and Replication of Heterotypic Model RNA Templates In Vivo

1999

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Influenza viruses type A, B, and C are human pathogens that share common structural and functional features, yet they do not form natural reassortants. To determine to what extent type-specific interactions of the polymerase complex with template RNA contribute to this lack of genotypic mixing, we investigated whether homotypic or heterotypic polymerase complexes support the expression and replication of model type A, B, or C RNA templates in vivo. A plasmid-based expression system, as initially described by Pleschka et al. [(1996) J. Virol. 70, 4188-4192] for influenza A virus, was developed for influenza viruses B/Harbin/7/94 and C/Johannesburg/1/66. The type A core proteins expressed heterotypic model RNAs with similar efficiencies as the homotypic RNA. The influenza B virus model RNA was efficiently expressed by all three types of polymerase complexes. Although no functional polymerase complex could be reconstituted with heterotypic P protein subunits, when the influenza A virus P proteins were expressed together with heterotypic nucleoproteins, significant, albeit limited, expression of RNA templates of all influenza virus types was detected. Taken together, our results suggest that less strict type-specific interactions are involved for the polymerase complex of influenza A compared with influenza B or C viruses.

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Section: Activity Of Homotypic Core Protein Complexes On Heterotypic mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of the Ability of the Polymerase Complexes of Influenza Viruses Type A, B and C to Assemble into Functional RNPs that Allow Expression and Replication of Heterotypic Model RNA Templates In Vivo

1999

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“…The majority of promoter studies were performed with influenza A virus; however, a transcription assay has also been established for influenza B virus [55]. Both viruses share similar features in their promoter structures.…”

Section: Influenza Virus Promotermentioning

confidence: 99%

“…Both viruses share similar features in their promoter structures. The influenza B virus promoter, like its influenza A virus counterpart, consists of two regions; region I, in which the nature of the nucleotide is critical, and region II, in which base-pairing is essential [55].…”

Section: Influenza Virus Promotermentioning

confidence: 99%

Generation of influenza A virus from cloned cDNAs — historical perspective and outlook for the new millenium

Kawaoka

2002

Reviews in Medical Virology

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Influenza virus reverse genetics has reached a level of sophistication where one can confidently generate virus entirely from cloned DNAs. The new systems makes it feasible to study the molecular mechanisms of virus replication and pathogenicity, as well as to generate attenuated live virus vaccines, gene delivery vehicles, and possibly other RNA viruses from cloned cDNAs. During the next decade, one can anticipate the translation of influenza virus reverse genetics into biomedically relevant advances.

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The Three Subunits of the Polymerase and the Nucleoprotein of Influenza B Virus Are the Minimum Set of Viral Proteins Required for Expression of a Model RNA Template

et al. 1997

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The genes encoding the nucleoprotein, PB1, PB2, and PA proteins of the influenza virus strain B/Panamá/45/90 have been cloned under control of the T7 RNA polymerase promoter of plasmid pGEM-3. Transfection of the recombinant plasmids obtained into mammalian cells, which had been infected with a vaccinia virus encoding the T7 RNA polymerase, resulted in expression of the expected influenza B virus polypeptides. Moreover, it is shown that coexpression of the four recombinant core proteins in COS-1 cells reconstituted a functional polymerase capable of expressing a synthetic influenza B virus-like CAT RNA. By using the influenza B virus recombinant plasmids and a set of pGEM-derived plasmids encoding the homologous core proteins of the influenza A virus A/Victoria/3/75 (I. Mena et al. (1994). J. Gen. Virol. 75, 2109-2114), the capabilities of homo- and heterotypic mixtures of the four core proteins to express synthetic type A and B CAT RNAs were analyzed. Both the influenza A and B virus polymerases were active in expressing, albeit with reduced efficiencies, the heterotypic model CAT RNAs. However, none of all possible heterotypic mixtures of the core proteins reconstituted a functional polymerase. In order to fully characterize the recombinant plasmids obtained, the nucleotide sequences of the cloned genes were determined and compared to sequences of other type B virus isolates. The results obtained from these latter analyses are discussed in terms of the conservation and evolution of the influenza B virus core genes.

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Mutational analysis of influenza B virus RNA transcription in vitro

Cited by 21 publications

References 28 publications

Comparative Analysis of the Ability of the Polymerase Complexes of Influenza Viruses Type A, B and C to Assemble into Functional RNPs that Allow Expression and Replication of Heterotypic Model RNA Templates In Vivo

Comparative Analysis of the Ability of the Polymerase Complexes of Influenza Viruses Type A, B and C to Assemble into Functional RNPs that Allow Expression and Replication of Heterotypic Model RNA Templates In Vivo

Generation of influenza A virus from cloned cDNAs — historical perspective and outlook for the new millenium

The Three Subunits of the Polymerase and the Nucleoprotein of Influenza B Virus Are the Minimum Set of Viral Proteins Required for Expression of a Model RNA Template

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