Mutational and transcriptional analysis of the Campylobacter jejuni flagellar biosynthesis gene flhB

Matz, Claudia; Vliet, Arnoud H. M. van; Ketley, Julian M.; Penn, Charles W.

doi:10.1099/00221287-148-6-1679

Cited by 16 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The C. jejuni flaA gene is transcribed from a 28 -specific promoter (35), and the C. jejuni flaB gene is transcribed from a 54 -specific promoter (16). A mutation was generated in the fliA gene of C. jejuni F38011, which encodes 28 , to determine whether this sigma factor is responsible for cia gene transcription. As predicted, the C. jejuni fliA mutant was nonmotile, most likely due to the lack of flaA transcription.…”

Section: Vol 186 2004 C Jejuni Protein Secretion 3299mentioning

confidence: 99%

“…This result indicates that the regulation of flagellar gene expression within C. jejuni differs from the regulation in more intensely studied systems such as that of Salmonella enterica. Unlike flagellar gene expression in C. jejuni, flagellar gene expression in S. enterica is initiated by a master regulator, while late gene expression and motility require 28 (1). Previous work in our laboratory has demonstrated that C. jejuni synthesizes a set of proteins during coculture with epithelial cells, a subset of which are secreted.…”

mentioning

confidence: 99%

“…The flagellar filament is comprised of two proteins, FlaA and FlaB, although it appears that FlaA is the preferred subunit (3). While the C. jejuni FlaA and FlaB flagellin proteins are transcribed concomitantly (16), the flaA gene is regulated by 28 and the flaB gene is regulated by 54 (3, 16). Hendrixson et al (16) noted that a C. jejuni isolate deficient in 28 , which is encoded by the fliA gene, is able to assemble a truncated filament composed exclusively of the flagellin protein FlaB.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Secretion of Virulence Proteins from Campylobacter jejuni Is Dependent on a Functional Flagellar Export Apparatus

et al. 2004

View full text Add to dashboard Cite

Campylobacter jejuni, a gram-negative motile bacterium, secretes a set of proteins termed the Campylobacter invasion antigens (Cia proteins). The purpose of this study was to determine whether the flagellar apparatus serves as the export apparatus for the Cia proteins. Mutations were generated in five genes encoding three structural components of the flagella, the flagellar basal body (flgB and flgC), hook (flgE2), and filament (flaA and flaB) genes, as well as in genes whose products are essential for flagellar protein export (flhB and fliI). While mutations that affected filament assembly were found to be nonmotile (Mot ؊ ) and did not secrete Cia proteins (S ؊ ), a flaA (flaB ؉ ) filament mutant was found to be nonmotile but Cia protein secretion competent (Mot ؊ , S ؉ ). Complementation of a flaA flaB double mutant with a shuttle plasmid harboring either the flaA or flaB gene restored Cia protein secretion, suggesting that Cia export requires at least one of the two filament proteins. Infection of INT 407 human intestinal cells with the C. jejuni mutants revealed that maximal invasion of the epithelial cells required motile bacteria that are secretion competent. Collectively, these data suggest that the C. jejuni Cia proteins are secreted from the flagellar export apparatus.Campylobacter jejuni, a gram-negative motile bacterium, is a frequent cause of human gastrointestinal infections (39). The spectrum of disease observed in C. jejuni-infected individuals ranges from asymptomatic to severe enteritis characterized by fever, severe abdominal cramping, and diarrhea with blood and mucus (2, 4). By analogy with other more extensively characterized bacterial pathogens, the mechanism of C. jejunimediated enteritis is proposed to be multifactorial. Previous work has indicated that motility as well as the presence of the flagellum contributes to the ability of C. jejuni to colonize the intestinal tract of animals (33,36,42).The flagellum of C. jejuni is composed of a basal body, hook, and filament. The flagellar filament is comprised of two proteins, FlaA and FlaB, although it appears that FlaA is the preferred subunit (3). While the C. jejuni FlaA and FlaB flagellin proteins are transcribed concomitantly (16), the flaA gene is regulated by 28 and the flaB gene is regulated by 54 (3, 16). Hendrixson et al. (16) noted that a C. jejuni isolate deficient in 28 , which is encoded by the fliA gene, is able to assemble a truncated filament composed exclusively of the flagellin protein FlaB. This result indicates that the regulation of flagellar gene expression within C. jejuni differs from the regulation in more intensely studied systems such as that of Salmonella enterica. Unlike flagellar gene expression in C. jejuni, flagellar gene expression in S. enterica is initiated by a master regulator, while late gene expression and motility require 28 (1). Previous work in our laboratory has demonstrated that C. jejuni synthesizes a set of proteins during coculture with epithelial cells, a subset of which are secreted. The sec...

show abstract

Section: Vol 186 2004 C Jejuni Protein Secretion 3299mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Secretion of Virulence Proteins from Campylobacter jejuni Is Dependent on a Functional Flagellar Export Apparatus

et al. 2004

View full text Add to dashboard Cite

show abstract

“…These included fliA and rpoN, which encode 28 and 54 , respectively; flgR, which encodes a 54 activator (14); and flhA and flhB, which have been implicated in the regulation of flagellar genes in campylobacter and in other organisms (26,27,31). PCR products of flagellar regulators generated from V1 and V26 were sequenced in both directions, including at least 200 nt of the upstream region.…”

Section: Levels Of Genes Encoding Specific Flagellar Transcripts and mentioning

confidence: 99%

“…Another key component involved in flagellar protein export is FlhB, which associates with FlhA and is involved in determining substrate specificity (30). In C. jejuni, FlhB has also been shown to be required for production of flagella and motility (29,31).…”

mentioning

confidence: 99%

Genome-wide Expression Analyses of Campylobacter jejuni NCTC11168 Reveals Coordinate Regulation of Motility and Virulence by flhA

Carrillo

Taboada

Nash

et al. 2004

Journal of Biological Chemistry

191

174

View full text Add to dashboard Cite

We examined two variants of the genome-sequenced strain, Campylobacter jejuni NCTC11168, which show marked differences in their virulence properties including colonization of poultry, invasion of Caco-2 cells, and motility. Transcript profiles obtained from whole genome DNA microarrays and proteome analyses demonstrated that these differences are reflected in late flagellar structural components and in virulence factors including those involved in flagellar glycosylation and cytolethal distending toxin production. We identified putative 28 and 54 promoters for many of the affected genes and found that greater differences in expression were observed for 28 -controlled genes. Inactivation of the gene encoding 28 , fliA, resulted in an unexpected increase in transcripts with 54 promoters, as well as decreased transcription of 28 -regulated genes. This was unlike the transcription profile observed for the attenuated C. jejuni variant, suggesting that the reduced virulence of this organism was not entirely due to impaired function of 28 . However, inactivation of flhA, an important component of the flagellar export apparatus, resulted in expression patterns similar to that of the attenuated variant. These findings indicate that the flagellar regulatory system plays an important role in campylobacter pathogenesis and that flhA is a key element involved in the coordinate regulation of late flagellar genes and of virulence factors in C. jejuni.

show abstract

Bidirectional transcription of lipooligosaccharide synthesis genes from Campylobacter jejuni

Phongsisay

Fry

2007

International Journal of Medical Microbiology

View full text Add to dashboard Cite

Mutational and transcriptional analysis of the Campylobacter jejuni flagellar biosynthesis gene flhB

Cited by 16 publications

References 39 publications

Secretion of Virulence Proteins from Campylobacter jejuni Is Dependent on a Functional Flagellar Export Apparatus

Secretion of Virulence Proteins from Campylobacter jejuni Is Dependent on a Functional Flagellar Export Apparatus

Genome-wide Expression Analyses of Campylobacter jejuni NCTC11168 Reveals Coordinate Regulation of Motility and Virulence by flhA

Bidirectional transcription of lipooligosaccharide synthesis genes from Campylobacter jejuni

Contact Info

Product

Resources

About