Mutations in gyrA and parC Genes in Quinolone-Resistant Klebsiella pneumoniae Isolates from Borujerd Hospitals

Shokoohizadeh, Leili; Saniee, Mehrangiz; Mirzaee, Mohsen; Taheri, Mohammad

doi:10.30699/jambs.27.120.1

Cited by 6 publications

(8 citation statements)

References 15 publications

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“…This comes around consistent with the reported rate by Shokoohizadeh et al (38%). 17 Our results for ciprofloxacin resistance in K. pneumoniae isolates (18.5%) were not in agreement with other reports, which have shown higher resistance. 18,19 Nevertheless, in previous studies performed by Razavi et al and Priyadarshini et al the prevalence of ciprofloxacin resistance was 19.6% and 19.09%, respectively, which was well in line with our results.…”

Section: Discussioncontrasting

confidence: 92%

“…In the present study, the rate of quinolones resistance was approximately 40%, and the majority of isolates were resistant to nalidixic acid and ofloxacin. This comes around consistent with the reported rate by Shokoohizadeh et al (38%) 17 . Our results for ciprofloxacin resistance in K. pneumoniae isolates (18.5%) were not in agreement with other reports, which have shown higher resistance 18,19 …”

Section: Discussionsupporting

confidence: 81%

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Investigation of plasmid‐mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae in southwest Iran

Jomehzadeh¹,

Ahmadi²,

Bahmanshiri³

2022

Clinical Laboratory Analysis

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Background Extensive and inappropriate use of quinolones has led to growing resistance rates to these broad‐spectrum antibiotics. The present study purposed to investigate the prevalence of plasmid‐mediated quinolone resistance (PMQR) genes in Klebsiella pneumoniae clinical isolates. Method Ninety‐two non‐repetitive K. pneumoniae clinical isolates were confirmed by standard microbiological methods. Antibacterial susceptibility of isolates toward seven agents from the quinolone family was evaluated by the disc diffusion method. Ciprofloxacin minimum inhibitory concentrations (MICs) were determined using the standard agar dilution method. PCR amplification was used to detect the existence of PMQR genes in the studied isolates. Results In the present study, significant quinolones' resistance (40%) was observed in K. pneumoniae isolates, and most of the strains were resistant to nalidixic acid (94.6%) and ofloxacin (45.6%). MIC analysis showed 15 strains were resistant to 6–128 μg/ml of ciprofloxacin, and five were intermediately‐resistant. PMQR genes were detected in 88% of all isolates. Acc(6’)‐Ib‐cr was constituted half of the total PMQR genes detected among ciprofloxacin non‐susceptible isolates. Of 20 ciprofloxacin non‐susceptible isolates, 65% (n = 13) harbored multiple PMQR determinants, and 15 strains were determined as integron carriage. Conclusion The findings of this study indicated considerable resistance against quinolones, which could be correlated with the extensive and inappropriate use of this class of antibiotics as empirical treatment.

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Section: Discussioncontrasting

confidence: 92%

Section: Discussionsupporting

confidence: 81%

Investigation of plasmid‐mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae in southwest Iran

Jomehzadeh¹,

Ahmadi²,

Bahmanshiri³

2022

Clinical Laboratory Analysis

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“…The resistance against quinolones is often originated from chromosomes due to mutation of DNA gyrase gene. This enzyme consists of four subunits; a sub-unit is the main target of quinolone group antibiotics (3,6). Unlike to abundance of global studies on quinolones and fluoroquinolones resistant K. pneumoniae, a few reports from Iran have shown the frequency of these resistance types and their genetic natures in clinical isolates of K. pneumoniae (6)(7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

“…This enzyme consists of four subunits; a sub-unit is the main target of quinolone group antibiotics (3,6). Unlike to abundance of global studies on quinolones and fluoroquinolones resistant K. pneumoniae, a few reports from Iran have shown the frequency of these resistance types and their genetic natures in clinical isolates of K. pneumoniae (6)(7)(8)(9)(10). Therefore, it is very important to continue studying the genes responsible for the resistance to different antibiotics in this pathogen particularly those are involved in quinolone resistance.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of gyrA Mutation in Clinical Strains of Klebsiella pneumoniae

Ahmadi¹,

Noormohammadi²,

Behzadi³

et al. 2022

ijph

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Background: Multi-drug resistant (MDR) Klebsiella pneumoniae strains cause the majority of community acquired and life-threatening infections. We aimed to detect the gyrA mutations in the clinical strains of nalidixic acid and ciprofloxacin resistant K. pneumoniae isolated from the patients with urinary tract infections. Methods: Bacterial strains were isolated from the patients with urinary tract infections admitted to a major hospital in Tehran, Iran (2017-2018). Bacterial identification was done according to standard microbiological tests. Antimicrobial susceptibility testing for quinolones and fluoroquinolone antibiotics was done using both disc diffusion and minimal inhibitory concentrations (MICs) methods. PCR-RFLP was used to detect the probable mutation in the gyrA gene in nalidixic acid and ciprofloxacin resistant strains. Finally sequencing was performed to detect point mutations in isolated K. pneumoniae strains. Results: One hundred K. pneumonia isolates were recovered from the urine samples of the clinical cases. Antibiotic resistance testing showed that among all K. pneumoniae isolates, 26% and 19% of the strains were resistant to nalidixic acid and ciprofloxacin respectively. MIC value was ≥4 µg/ml for ciprofloxacin resistant isolates. The results of RFLP on gyrA PCR amplicons using HinfI restriction enzyme showed point mutation in this gene in 46% of nalidixic acid and ciprofloxacin resistant K. pneumonia. The data obtained from the sequencing confirmed the RFLP results and indicated the presence of point mutations in codons 83 and 87 in the gyrA gene which leads to the substitution of different amino acids in gyrA protein. Conclusion: Our findings indicated a relative increased rate of resistance against quinolones and fluoroquinolone antibiotics that raised a concern about extensive dissemination of clinical strains of nalidixic acid and ciprofloxacin resistant K. pneumonia. Point mutation of gyrA gene was responsible for the resistance in our strains however to gain more insight into the molecular characterization of quinolone-resistant isolates, other possible mechanisms of the resistance should also be investigated.

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“…Sulfonamides are antibiotics with a broad spectrum of action that are made chemically and used to treat bacterial infections [ 10 ]. In sulfonamide resistant strains, sul genes have been detected and provide translocation on chromosomes and plasmids are often associated with MGEs [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of mobile genetic elements in multidrug-resistant Klebsiella pneumoniae isolated from different infection sites in Hamadan, west of Iran

et al. 2021

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Objective Klebsiella pneumoniae is one of most opportunistic pathogens that can be related to nosocomial infections. Increased acquisitions of multidrug resistance in this bacterium as well as the transfer of genes to other strains have caused concern. Integrons play key role in the acquisition and the spread of resistance genes. The aim of this study was evaluated the frequency of resistance genes sulI, sulII, tetA, tetB, class I (intI gene), class II integrons (intII gene) and the association between multidrug resistance and the presence of integrons in K. pneumoniae. Results Antibiotics susceptibility test was performed on 126 of K. pneumoniae isolates. Also, DNA extraction was done and genes were detected using PCR method. In this study, 67 isolates (53%), carrying both the sulI and sulII genes. Forty-five percent tetracycline-resistant isolates were tetA or tetB positive. The prevalence of intI gene was 96%, while only sixteen isolate harboring intII gene (12.5%). Our results showed the high prevalence of integrons in MDR K. pneumoniae, indicating the important role of these genes in the transmission of antibiotic resistance.

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Mutations in gyrA and parC Genes in Quinolone-Resistant Klebsiella pneumoniae Isolates from Borujerd Hospitals

Cited by 6 publications

References 15 publications

Investigation of plasmid‐mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae in southwest Iran

Investigation of plasmid‐mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae in southwest Iran

Molecular Detection of gyrA Mutation in Clinical Strains of Klebsiella pneumoniae

Detection of mobile genetic elements in multidrug-resistant Klebsiella pneumoniae isolated from different infection sites in Hamadan, west of Iran

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