Mutations in the F‐box gene <i>LARGER PANICLE</i> improve the panicle architecture and enhance the grain yield in rice

Li, Ming; Tang, Ding; Wang, Kejian; Wu, Xinru; Lu, Lili; Yu, Hengxiu; Gu, Minghong; Yan, Chuan; Cheng, Zhihui

doi:10.1111/j.1467-7652.2011.00610.x

Cited by 164 publications

(111 citation statements)

References 51 publications

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“…The Kelch domain has been reported to represent one β-sheet blade, and several repeats are able to interact to form a β-propeller (17). In rice, LARGER PANICLE (LP) encoding a Kelch repeat-containing F-box protein was reported to play a negative role in regulating plant architecture, particularly panicle architecture (18). In this study, we also found that the Kelch domains in OsPPKL1 93-11 play a negative regulation role in forming grain length.…”

Section: Resultsmentioning

confidence: 99%

Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice

Zhang

Wang

Huang

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

428

315

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Grain size and shape are important components determining rice grain yield, and they are controlled by quantitative trait loci (QTLs). Here, we report the cloning and functional characterization of a major grain length QTL, qGL3, which encodes a putative protein phosphatase with Kelch-like repeat domain (OsPPKL1). We found a rare allele qgl3 that leads to a long grain phenotype by an aspartate-to-glutamate transition in a conserved AVLDT motif of the second Kelch domain in OsPPKL1. The rice genome has other two OsPPKL1 homologs, OsPPKL2 and OsPPKL3. Transgenic studies showed that OsPPKL1 and OsPPKL3 function as negative regulators of grain length, whereas OsPPKL2 as a positive regulator. The Kelch domains are essential for the OsPPKL1 biological function. Field trials showed that the application of the qgl3 allele could significantly increase grain yield in both inbred and hybrid rice varieties, due to its favorable effect on grain length, filling, and weight.QTL mapping | positional cloning | Oryza sativa L.

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Section: Resultsmentioning

confidence: 99%

Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice

Zhang

Wang

Huang

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

428

315

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“…We observed that Gn1a (OsCKX2), encoding a cytokinin oxidase/dehydrogenase that regulates grain number by degrading bioactive cytokinin in inflorescence meristems (22), was strongly selected in IndI. LARGER PANICLE (LP), a gene that encodes a Kelch repeat-containing F-box protein (23), was also found to be significantly selected in IndI. In connection with the previous report that two allelic lp mutants showed an increased panicle size with more grains, it was suggested that LP might also be a very important target for improvement in rice breeding.…”

Section: Significancementioning

confidence: 99%

Breeding signatures of rice improvement revealed by a genomic variation map from a large germplasm collection

Xie

Wang

Yuan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

189

170

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Intensive rice breeding over the past 50 y has dramatically increased productivity especially in the indica subspecies, but our knowledge of the genomic changes associated with such improvement has been limited. In this study, we analyzed low-coverage sequencing data of 1,479 rice accessions from 73 countries, including landraces and modern cultivars. We identified two major subpopulations, indica I (IndI) and indica II (IndII), in the indica subspecies, which corresponded to the two putative heterotic groups resulting from independent breeding efforts. We detected 200 regions spanning 7.8% of the rice genome that had been differentially selected between IndI and IndII, and thus referred to as breeding signatures. These regions included large numbers of known functional genes and loci associated with important agronomic traits revealed by genome-wide association studies. Grain yield was positively correlated with the number of breeding signatures in a variety, suggesting that the number of breeding signatures in a line may be useful for predicting agronomic potential and the selected loci may provide targets for rice improvement.) is one of the most important cereal crops in the world. There have been landmark achievements in rice improvement over the past 50 y, especially in the indica subspecies. A major breakthrough resulted from the independent development of a series of semidwarf varieties in China and by the International Rice Research Institute (IRRI) in the 1950s and 1960s, leading to the "green revolution" in rice. Since then, semidwarfness has been a basic characteristic for almost all modern varieties. Based on semidwarf varieties, improvement for other traits, such as abiotic stress resistance, broad-spectrum resistances to biotic stresses, and better grain quality, has also been achieved. Another major breakthrough stemmed from the exploitation of hybrid vigor in China (1), resulting in the largescale adoption of hybrid rice since the 1970s. Jointly, these breakthroughs have greatly increased rice productivity in the past several decades globally.Genomic studies in recent years have identified a large number of loci that were under selection during rice domestication (2). However, there has been very limited study to identify loci or genomic regions that have been under selection due to breeding. Next-generation sequencing technologies have enabled sequencing of a large number of rice accessions at relatively low cost, providing opportunities to inspect the genomic regions selected in the history of crop improvement. Meanwhile, genome-wide association studies (GWAS) have provided an effective approach to analyze the genetic architecture of complex traits and allow identification of candidate genes for further improvement of agronomically important traits (3,4).In this study, we analyzed low-coverage sequencing data of 1,479 rice accessions, which revealed a large number of differentially selected regions associated with breeding efforts between two major subpopulations in indica. These selected regi...

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“…The FBK proteins ZEITLUPE, FLAVIN-BINDING, KELCH REPEAT, F-BOX1, and LOV KELCH PROTEIN2 are involved in light signaling, flowering, and circadian control via a proteasome-dependent pathway in Arabidopsis (Imaizumi et al, 2005). The rice (Oryza sativa) FBK gene LARGER PANICLE/ERECT PANICLE3 was reported to regulate panicle architecture (Piao et al, 2009) and modulate cytokinin levels through Oryza sativa CYTOKININ OXIDASE2 expression (Li et al, 2011a). However, only a few substances for F-box proteins with specific functions are known in plants.…”

mentioning

confidence: 99%

The F-Box Protein OsFBK12 Targets OsSAMS1 for Degradation and Affects Pleiotropic Phenotypes, Including Leaf Senescence, in Rice

et al. 2013

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Leaf senescence is related to the grain-filling rate and grain weight in cereals. Many components involved in senescence regulation at either the genetic or physiological level are known. However, less is known about molecular regulation mechanisms. Here, we report that OsFBK12 (an F-box protein containing a Kelch repeat motif) interacts with S-ADENOSYL-L-METHIONINE SYNTHETASE1 (SAMS1) to regulate leaf senescence and seed size as well as grain number in rice (Oryza sativa). Yeast two-hybrid, pull-down, and bimolecular fluorescence complementation assays indicate that OsFBK12 interacts with Oryza sativa S-PHASE KINASE-ASSOCIATED PROTEIN1-LIKE PROTEIN and with OsSAMS1. Biochemical and physiological data showed that OsFBK12 targets OsSAMS1 for degradation. OsFBK12-RNA interference lines and OsSAMS1 overexpression lines showed increased ethylene levels, while OsFBK12-OX lines and OsSAMS1-RNA interference plants exhibited decreased ethylene. Phenotypically, overexpression of OsFBK12 led to a delay in leaf senescence and germination and increased seed size, whereas knockdown lines of either OsFBK12 or OsSAMS1 promoted the senescence program. Our results suggest that OsFBK12 is involved in the 26S proteasome pathway by interacting with Oryza sativa S-PHASE KINASE-ASSOCIATED PROTEIN1-LIKE PROTEIN and that it targets the substrate OsSAMS1 for degradation, triggering changes in ethylene levels for the regulation of leaf senescence and grain size. These data have potential applications in the molecular breeding of rice.

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Mutations in the F‐box gene LARGER PANICLE improve the panicle architecture and enhance the grain yield in rice

Cited by 164 publications

References 51 publications

Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice

Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice

Breeding signatures of rice improvement revealed by a genomic variation map from a large germplasm collection

The F-Box Protein OsFBK12 Targets OsSAMS1 for Degradation and Affects Pleiotropic Phenotypes, Including Leaf Senescence, in Rice

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