Mutations in the Saccharomyces cerevisiae vacuolar fusion proteins Ccz1, Mon1 and Ypt7 cause defects in cell cycle progression in a num1Δ background

CCZ1,MON1andYPT7genes are involved in pexophagy, the Cvt pathway and non-specific macroautophagy in the methylotrophic yeastPichia pastoris

“…cerevisiae Mon1 and Ccz1 proteins are involved in pexophagy, autophagy and Cvt biosynthetic pathways at the late stages of vacuole delivery (Meiling-Wesse et al, 2002;Wang et al, 2002). Ypt7 acts in complex with Ccz1 and Mon1 and is thus required for correct fusion of vesicles with the vacuole (Kucharczyk et al, 2001;Hoffman-Sommer et al, 2009). We found that the putative orthologues of P. pastoris had 23% identity and 43% similarity to Ccz1p ( Figure 1A), 33% identity and 53% similarity to Mon1p ( Figure 1B) and 67% identity and 84% similarity to Ypt7p of S. cerevisiae ( Figure 1C).…”

“…S. Ypt7 acts in complex with Ccz1 and Mon1 and is thus required for correct fusion of vesicles with the vacuole(Kucharczyk et al, 2001;Hoffman-Sommer et al, 2009). Ypt7 acts in complex with Ccz1 and Mon1 and is thus required for correct fusion of vesicles with the vacuole(Kucharczyk et al, 2001;Hoffman-Sommer et al, 2009).…”

Mon1a Protein Acts in Trafficking through the Secretory Apparatus

“…Proteins in the immunoprecipitate were identified using Western blot analysis as described above, and mass spectrometry was performed at the University of Utah Mass Spectrometry Core as described previously (16). (1)(2)(3). More recently, Mon1 and its partner Ccz1 have been shown to act as a guanine nucleotide exchange factor of the late endosomal rab7 homolog Ypt7 in S. cerevisiae (17).…”