Mutations in zucchini yellow mosaic virus helper component protein associated with loss of aphid transmissibility

Granier, Fabienne; Durand-Tardif, Mylène; Casse-Delbart, Francine; Lecoq, Hervé; Robaglia, Christophe

doi:10.1099/0022-1317-74-12-2737

Cited by 53 publications

(36 citation statements)

References 30 publications

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“…The presence of a lysine at position 50, a residue shown to be important in HC-Pro-mediated aphid transmission of potyviruses (Atreya et al, 1992;Atreya & Pirone, 1993), is in agreement with the aphidtransmissible phenotype of this virus (K. Grbrr-S~lassi~, personal communication). No amino acid alterations were observed in regions known to be highly conserved between potyviruses (Granier et al, 1993), except the central CCC box described by Cronin et al (1995) which was CSC in PVY LYE84.…”

Section: Cdna Sequencementioning

confidence: 93%

“…The N-terminal portion of HC-Pro is required for aphidmediated plant-to-plant transmission (Atreya et al, 1992;Atreya & Pirone, 1993). However, in a recent report a point mutation within the conserved C-terminal proline-threonine-lysine (PTK) box of HC-Pro was shown to be associated with loss of aphid transmissibility of zucchini yellow mosaic potyvirus (Granier et al, 1993;Huet et al, 1994), suggesting that more than one site in HC-Pro could be involved in aphid transmission activity.…”

Section: Introductionmentioning

confidence: 99%

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Nucleic acid-binding properties of a bacterially expressed potato virus Y helper component-proteinase

Maia

Bernardi

1996

Journal of General Virology

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The potyvirus helper component-proteinase (HC-Pro) is a multifunctional protein previously reported to have affinity for polyribonucleotides. To investigate further the ability of HC-Pro to bind nucleic acids, the potato virus Y (PVY) LYE84 isolate HC-Pro gene was amplified, cloned in an Escherichia coli expression vector and sequenced. HC-Pro was expressed as a fusion with the maltose-binding protein and purified by affinity chromatography. Electrophoretic mobility-shift assays demonstrated that HC-Pro acts as a sequence nonspecific RNA-binding protein and suggest that more than one molecule of protein was bound per molecule of RNA. The HC-Pro RNA-binding activity was stable in 400 mu-NaC1 and temperature sensitive. The recombinant protein preferentially bound ssRNA over DNA or dsRNA and showed little, if any, affinity for poly(A). The possible implications of the RNA-binding activity of HC-Pro in potyvirus replication and movement are discussed.

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Section: Cdna Sequencementioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Nucleic acid-binding properties of a bacterially expressed potato virus Y helper component-proteinase

Maia

Bernardi

1996

Journal of General Virology

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“…The same mutation was also found in a helper-deficient strain of ZYMV from Connecticut (Grumet et al, 1992). For another ZYMV isolate, two mutations were found between the HAT and PAT strains, one of them occurring in a conserved cluster of amino-acids Pro-Thr-Lys (PTK) (Granier et al, 1993). Huet et al (1994) modified the PTK of ZYMV to PAK in an infectious cDNA clone and observed a total loss of HC activity in aphid transmission.…”

Section: Aphid Transmissionmentioning

confidence: 99%

“…Loss of aphid transmissibility can result from a deficiency of the CP (Antignus et al, 1989;Lee et al, 1993) or from the lack of biologically active HC (Lecoq et al, 1991a;Granier et al, 1993).…”

Section: Aphid Transmissionmentioning

confidence: 99%

“…These strains, named PAT (poorly aphid transmissible), are deficient in their helper component activity. Granier et al (1993) compared the helper component sequences of two PAT strains to that of the highly aphid transmissible (HAT) strains from which they derived. They observed in one case a K to E mutation in the N-terminal part of the HC similar to that observed in the PVC aphid nontransmissible variant of potato virus Y (PVY) (Thornbury et al, 1990).…”

Section: Aphid Transmissionmentioning

confidence: 99%

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Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

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224

172

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Zucchini yellow mosaic potyvirus (ZYMV), first isolated in Italy in 1973, described in 1981, and then identified in all continents within a decade, is one of the most economically important viruses of cucurbit crops. It is efficiently aphid-transmitted in a nonpersistent manner and it is also seed-borne in zucchini squash, which could have contributed to its rapid spread worldwide. Biological variability has been observed among ZYMV isolates, concerning host range, symptomatology and aphid transmissibility. More recent studies also revealed a serological and molecular variability. The survival of ZYMV in areas where cucurbits are not grown throughout the year remains to be elucidated, because very few natural over-wintering hosts have been identified so far. Partial control of ZYMV can be achieved by limiting transmission of the virus to the crops by aphids, using adapted cultural practices. Cross-protection with a mild strain has been shown to be effective against most ZYMV isolates. Resistance genes found in cucurbit germplasms are currently being introduced into cultivars with good agronomical characteristics. Pathogen-derived resistance strategies using the expression of ZYMV genes in transgenic plants have also been developed and appear promising. Nevertheless, the high biological variability of ZYMV justifies a careful evaluation of the deployment of genetic control strategies in order to increase their durability.

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Multiple Interactions among Proteins Encoded by the Mite-Transmitted Wheat Streak Mosaic Tritimovirus

2000

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The genome organization of the mite-transmitted wheat streak mosaic virus (WSMV) appears to parallel that of members of the Potyviridae with monopartite genomes, but there are substantial amino acid dissimilarities with other potyviral polyproteins. To initiate studies on the functions of WSMV-encoded proteins, a protein interaction map was generated using a yeast two-hybrid system. Because the pathway of proteolytic maturation of the WSMV polyprotein has not been experimentally determined, random libraries of WSMV cDNA were made both in DNA-binding domain and activation domain plasmid vectors and introduced into yeast. Sequence analysis of multiple interacting pairs revealed that interactions largely occurred between domains within two groups of proteins. The first involved interactions among nuclear inclusion protein a, nuclear inclusion protein b, and coat protein (CP), and the second involved helper component-proteinase (HC-Pro) and cylindrical inclusion protein (CI). Further immunoblot and deletion mapping analyses of the interactions suggest that subdomains of CI, HC-Pro, and P1 interact with one another. The two-hybrid assay was then performed using full-length genes of CI, HC-Pro, P1, P3, and CP, but no heterologous interactions were detected. In vitro binding assay using glutathione-S-transferase fusion proteins and in vitro translation products, however, revealed mutual interactions among CI, HC-Pro, P1, and P3. The failure to detect interactions between full-length proteins by the two-hybrid assay might be due to adverse effects of expression of viral proteins in yeast cells. The capacity to participate in multiple homomeric and heteromeric molecular interactions is consistent with the pleiotropic nature of many potyviral gene mutants and suggests mechanisms for regulation of various viral processes via a network of viral protein complexes.

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Mutations in zucchini yellow mosaic virus helper component protein associated with loss of aphid transmissibility

Abstract: Zucchini yellow mosaic virus (ZYMV) is a potyvirustransmitted by aphids in a non-persistent manner.

Cited by 53 publications

References 30 publications

Nucleic acid-binding properties of a bacterially expressed potato virus Y helper component-proteinase

Nucleic acid-binding properties of a bacterially expressed potato virus Y helper component-proteinase

Zucchini yellow mosaic virus

Multiple Interactions among Proteins Encoded by the Mite-Transmitted Wheat Streak Mosaic Tritimovirus

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