Mutations inside rifampicin-resistance determining region of rpoB gene associated with rifampicin-resistance in Mycobacterium tuberculosis

Zaw, Myo Thura; Emran, Nor Amalina; Wang, Qisheng

doi:10.1016/j.jiph.2018.04.005

Cited by 153 publications

(136 citation statements)

References 29 publications

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“…In RRDR, the most commonly mutated codons are 512, 513, 516, 526, 531. In addition, mutations outside RRDR were also detected in Rif-resistant strains (Zaw et al, 2018). In this study, we found that A650 and L4650 strains had changes in the codon in RRDR (Gln513Pro, Val524Ile).…”

Section: Discussionmentioning

confidence: 62%

“…The full-length of the rpoB gene was 4029 bp encoding a polypeptide with 1342 amino acids (Campbell et al, 2001). Mutations in the rpoB gene were found to be associated with Rif resistance of some bacterial strains such as Mycobacterium tuberculosis, Escherichia coli, Acinetobacter baumannii, Bacillus subtilis, V. parahaemolyticus (Hazen et al, 2009;Jin and Gross, 1988;Maughan et al, 2004;Perez-Varela et al, 2017;Zaw et al, 2018), and the mutation position would affect the level of the resistance to Rif (Taniguchi et al, 1996). Hazen et al (2009) reported that V. parahaemolyticus Rif-resistant strains with concentration at 100 µg/ml had the mutations in the rpoB gene (Hazen et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Hazen et al (2009) reported that V. parahaemolyticus Rif-resistant strains with concentration at 100 µg/ml had the mutations in the rpoB gene (Hazen et al, 2009). The region which contains 81 bp from codon 507 to codon 533 (27 amino acid) is called rifampicinresistance determining region (RRDR) (Zaw et al, 2018). In RRDR, the most commonly mutated codons are 512, 513, 516, 526, 531.…”

Section: Discussionmentioning

confidence: 99%

“…The rpoB sequence of the L4650 strain had changes in some nucleotide positions compared to the wild-type strain, which resulted in changes of DaaSeq in 28 positions (Table 3). The changed codon 524 was located in 81 bp rifampicin-resistance determining region (RRDR) (Zaw et al, 2018). No nucleotide change was found in rpoB sequences of three attenuated strains (B5700, B7050, B7900) compared with wild-type strain (B3.13) in the second group.…”

Section: The Change Of Rpob Gene Sequencementioning

confidence: 96%

“…In the third group, only A650 strain had a nucleotide change leading to a replacement of amino acid in codon 513 (Gln513Pro). It was one hotspot of mutation associated with Rif resistance among many bacteria species (Jin and Gross, 1988;Lee et al, 2005;Maughan et al, 2004;Zaw et al, 2018). The A400 strain had no change nucleotide sequence of the rpoB gene region compared with the A3.3 strain.…”

Section: The Change Of Rpob Gene Sequencementioning

confidence: 99%

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Development of attenuated Vibrio parahaemolyticus mutant strains as potential live vaccines

Huyen

Viết

Tam

et al. 2020

APJMBB

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Vibrio parahaemolyticus is a pathogenic Gram-negative bacterium that commonly infects numerous species of marine fishes. In this study, we obtained eight attenuated mutant strains by the selection of rifampicin-resistant V. parahaemolyticus from three wild-type strains. Among eight selected strains, four strains (L4650, A650, L4200 and A400) were widely attenuated by virulence analysis. Sequences of two genes, tlh and toxR, that related to virulence were analyzed. The results showed that seven attenuated strains were different in sequences of the tlh gene while there was no difference in sequence of toxR gene in comparison with that of wild-type strains. Three attenuated strains (L4650, L4200 and A650) had the nucleotide changes in tlh gene that resulted to the changes in the size of deduced amino acid sequences. In addition, rpoB gene coding for -subunit of RNA polymerase that related to Rifampicin resistance was analyzed for all selected strains. Two strains (A650 and L4650) had the nucleotide changes in ropB sequence. To assess the potential use of the L4650 strain as a live attenuated vaccine, the groupers (Epinephelus coioides) were intraperitoneally injected with the L4650 strain and later challenged with the virulent wild-type strain at three different doses. Results showed the protection for L4650 strain vaccinated-fish produced relatively earlier than usual at 15 days post-vaccination with relative percent survival (RPS) values from 96.91-100%. At 60 days post-vaccination, the RPS values of vaccinated fish were 96.27-100%. Our results suggest the L4650 strain could become a potential vaccine candidate against V. parahaemolyticus for future studies.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: The Change Of Rpob Gene Sequencementioning

confidence: 96%

Section: The Change Of Rpob Gene Sequencementioning

confidence: 99%

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Development of attenuated Vibrio parahaemolyticus mutant strains as potential live vaccines

Huyen

Viết

Tam

et al. 2020

APJMBB

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Truenat MTB assays for pulmonary tuberculosis and rifampicin resistance in adults

Inbaraj

Daniel

Rajendran

et al. 2023

Cochrane Database of Systematic Reviews

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Synthesis and in vitro Metabolic Stability of Sterically Shielded Antimycobacterial Phenylalanine Amides

Lang,

Ganapathy,

Mann

et al. 2024

ChemMedChem

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Nα‐aroyl‐N‐aryl‐phenylalanine amides (AAPs) are RNA polymerase inhibitors with activity against Mycobacterium tuberculosis and non‐tuberculous mycobacteria. We observed that AAPs rapidly degrade in microsomal suspensions, suggesting that avoiding hepatic metabolism is critical for their effectiveness in vivo. As both amide bonds are potential metabolic weak points of the molecule, we synthesized 16 AAP analogs in which the amide bonds are shielded by methyl or fluoro substituents in close proximity. Some derivatives show improved microsomal stability, while being plasma‐stable and non‐cytotoxic. In parallel with the metabolic stability studies, the antimycobacterial activity of the AAPs against Mycobacterium tuberculosis, Mycobacterium abscessus, Mycobacterium avium and Mycobacterium intracellulare was determined. The stability data are discussed in relation to the antimycobacterial activity of the panel of compounds and reveal that the concept of steric shielding of the anilide groups by a fluoro substituent has the potential to improve the stability and bioavailability of AAPs.

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Mutations inside rifampicin-resistance determining region of rpoB gene associated with rifampicin-resistance in Mycobacterium tuberculosis

Abstract: Molecular methods which can identify extensive mutations associated with multiple anti-tuberculous drugs are in urgent need so that the research on drug resistant mutations should be extended.

Cited by 153 publications

References 29 publications

Development of attenuated Vibrio parahaemolyticus mutant strains as potential live vaccines

Development of attenuated Vibrio parahaemolyticus mutant strains as potential live vaccines

Truenat MTB assays for pulmonary tuberculosis and rifampicin resistance in adults

Synthesis and in vitro Metabolic Stability of Sterically Shielded Antimycobacterial Phenylalanine Amides

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