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Nowadays, the percentage of people with cancer is increasing. There are many methods used to treat cancer such as surgery, chemotherapy, the radiation, etc. In addition, the use of medicinal plants, natural products, is also popularly applied for cancer patients. Using 90% ethanol as the extraction solvent, we produced 4 kinds of concentrated extracts from dried flowers, dried leaves, fresh flowers, fresh leaves of male papaya (Carica papaya Linn). In vitro study, the extracts of dried flowers, fresh flowers did not effect on MCF7, A549, HT 29, Huh 7R, HEK 293 and LLC cancer cell lines. The extracts of dried leaves and fresh leaves presented cytotoxic effects with IC50 values ranging from 1.88 to 13.64 mg/mL. Meanwhile, activities of the extract of fresh leaves was 4-6 times stronger than that of the extract of dried leaves's. Based on evaluation of the IL2 and IL6 production capability of macrophage, we found that the extract of fresh leaves strongly inhibited the IL-6 production at 4 mg/mL (50.56%) and 0.8 mg/mL (23.79%) in comparison with the negative control (P<0.05) while it did not show the ability to induce IL-2 in the same studied concentrations. Using tumor bearing mice as model for studying the antitumor activities of extract of fresh leaves at the dose of 3 mL/kg/day and 6 mL/kg/day, we identified the positive effects of the higher dose treatment. The results showed that the extract of fresh leaves at the dose of 6 mL/kg/day could inhibit 33.44% the tumors’ sizes on day 28 (P<0.05); tumor's weigh decreased to 9.84 against 13.76 g of the negative control; the WBC parameter reduced from 41.65x106/mL to 19.1x106/mL (P<0.05), AST decreased from 1441.6 U/L to 1038.97 U/L (P<0.01), and creatinin decreased from 26.37 µmol/L to 20.63 µmol/L compared with the negative control group (P<0.05). These promising results are the scientific evidences for applying this extract in cancer treatment in the future.

In this study, we aimed at evaluating in vitro and in vivo anti-inflammatory activity of various extracts of the rhizomes of Globba pendula Roxb. Three extracts ( n-hexane, ethyl acetate, and water) were screened for their inhibitory effect on NO production by lipopolysaccharide-stimulated RAW 264.7 macrophages. The ethyl acetate extract of G. pendula rhizomes (EGP) showed a potential effect with an IC50 value of 32.45 µg/mL. For in vivo study, the ethyl acetate extract was further investigated for its anti-inflammatory effect using collagen antibody-induced arthritic mice (CAIA). The level of arthritis in experimental mice significantly reduced ( P < .05) after treatment with EGP at a dose of 500 mg/kg body weight (b.w.). This study also revealed that EGP is orally non-toxic. Ethyl p-methoxy cinamate was identified as the main constituent of EGP, which may result in its anti-inflammatory effect.

Tóm tắt: Một thí nghiệm 2 yếu tố đã được bố trí theo kiểu khối đầy đủ ngẫu nhiên với 3 lần lặp lại trên cây xà lách (Lactuca sativa var. capitata L.) trồng tại tỉnh Gia Lai. Yếu tố A là 2 mức đạm bón: (1) 60 kg/ha, (2) 80 kg/ha; yếu tố B là phương thức sử dụng HB 101: (1) không sử dụng HB 101 – Phun nước lã (ĐC), (2) sử dụng HB 101 tưới vào đất, (3) sử dụng HB 101 tưới vào đất + phun lên cây 3 lần; và (4) sử dụng HB 101 phun lên cây 3 lần. Mục tiêu của nghiên cứu là tìm liều lượng đạm bón và phương thức sử dụng HB 101 phù hợp cho sinh trưởng và năng suất của xà lách được trồng tại tỉnh Gia Lai. Kết quả nghiên cứu cho thấy cây xà lách trồng tại Gia Lai được bón phân đạm với liều lượng 60 kg/ha và phun HB 101 lên cây 3 lần cho sinh trưởng tốt nhất. Năng suất thực tế và năng suất thương phẩm đạt cao nhất lần lượt là 3.547,0 kg/1000 m2 và 2.581,7 kg/1000 m2, cho lợi nhuận cao nhất (13.367.900 đ/1000 m2) và tỷ suất lợi nhuận cao nhất đạt 2,84.Từ khóa: HB 101, phân đạm, xà lách