Mutations of flagellar genesfliC12,fliAandflhDCofEdwardsiella tardaattenuated bacterial motility, biofilm formation and virulence to fish

Xu, Tingting; Su, Ying; Ying, Xu; He, Yang; Wang, Bei; Dong, Xue; Li, Y.; Zhang, X.-H.

doi:10.1111/jam.12357

Cited by 33 publications

(25 citation statements)

References 34 publications

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“…, ; Xu et al. , ). In D. dadantii , FlhDC has recently been shown to control, in addition to flagellar motility, a type III secretion system and virulence factor synthesis through several pathways (Yuan et al.…”

Section: Resultsmentioning

confidence: 99%

Identification by Tn‐seq of Dickeya dadantii genes required for survival in chicory plants

Royet

Parisot

Rodrigue

et al. 2018

Molecular Plant Pathology

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Summary The identification of the virulence factors of plant‐pathogenic bacteria has relied on the testing of individual mutants on plants, a time‐consuming process. Transposon sequencing (Tn‐seq) is a very powerful method for the identification of the genes required for bacterial growth in their host. We used this method in a soft‐rot pathogenic bacterium to identify the genes required for the multiplication of Dickeya dadantii in chicory. About 100 genes were identified showing decreased or increased fitness in the plant. Most had no previously attributed role in plant–bacterium interactions. Following our screening, in planta competition assays confirmed that the uridine monophosphate biosynthesis pathway and the purine biosynthesis pathway were essential to the survival of D. dadantii in the plant, as the mutants ∆ carA , ∆ purF , ∆ purL , ∆ guaB and ∆ pyrE were unable to survive in the plant in contrast with the wild‐type (WT) bacterium. This study also demonstrated that the biosynthetic pathways of leucine, cysteine and lysine were essential for bacterial survival in the plant and that RsmC and GcpA were important in the regulation of the infection process, as the mutants ∆ rsmC and ∆ gcpA were hypervirulent. Finally, our study showed that D. dadantii flagellin was glycosylated and that this modification conferred fitness to the bacterium during plant infection. Assay by this method of the large collections of environmental pathogenic strains now available will allow an easy and rapid identification of new virulence factors.

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Section: Resultsmentioning

confidence: 99%

Identification by Tn‐seq of Dickeya dadantii genes required for survival in chicory plants

Royet

Parisot

Rodrigue

et al. 2018

Molecular Plant Pathology

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“…The pathogenesis of this species and its disease-causing mechanisms remain largely unknown. However, recent studies on E. tarda have identified a number of potential virulence factors associated with the pathogenicity of this bacterium (Yang et al 2012;Xu et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production

Castro

Osorio

Buján

et al. 2015

Journal of Fish Diseases

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Edwardsiella tarda has long been known as a pathogen that causes severe economic losses in aquaculture industry. Insights gained on E. tarda pathogenesis may prove useful in the development of new methods for the treatment of infections as well as preventive measures against future outbreaks. In this report, we have established the correlation between the presence of virulence genes, related with three aspects typically involved in bacterial pathogenesis (chondroitinase activity, quorum sensing and siderophore-mediated ferric uptake systems), in the genome of E. tarda strains isolated from turbot in Europe and their phenotypic traits. A total of 8 genes were tested by PCR for their presence in 73 E. tarda isolates. High homogeneity was observed in the presence/absence pattern of all the strains. Positive results in the amplification of virulence-related genes were correlated with the detection of chondroitinase activity in agar plates, in vivo AHL production during fish infection and determination of type of siderophore produced by E. tarda. To the best of our knowledge, this is the first study carried out with European strains on potential virulence factors. Furthermore, we demonstrated for the first time that E. tarda produces the siderophore vibrioferrin.

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“…Accumulating evidences indicate that the motility of bacteria plays an important role in biofilm development (Wood et al, 2006; Meshcheryakov et al, 2013). For example, E. coli lacking flagella or possessing paralyzed flagella is defective in biofilm growth (Wood et al, 2006; Houry et al, 2010), and Edwardsiella tarda with impaired flagella exhibited reduced biofilm production (Xu et al, 2014). Biofilms are multicellular aggregates of bacteria bound by a matrix of extracellular polymers (O’Toole et al, 2000; Branda et al, 2006; Monds and O’Toole, 2009).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity

Sun¹,

Chi

Sun

2016

Front. Microbiol.

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Pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. In this study, we employed proteomic analysis and identified filamentous hemagglutinin (FHA) as an iron-responsive protein secreted by TSS, a pathogenic P. fluorescens isolate. In vitro study showed that compared to the wild type, the fha mutant TSSfha (i) exhibited a largely similar vegetative growth profile but significantly retarded in the ability of biofilm growth and producing extracellular matrix, (ii) displayed no apparent flagella and motility, (iii) was defective in the attachment to host cells and unable to form self-aggregation, (iv) displayed markedly reduced capacity of hemagglutination and surviving in host serum. In vivo infection analysis revealed that TSSfha was significantly attenuated in the ability of dissemination in fish tissues and inducing host mortality, and that antibody blocking of the natural FHA produced by the wild type TSS impaired the infectivity of the pathogen. Furthermore, when introduced into turbot as a subunit vaccine, recombinant FHA elicited a significant protection against lethal TSS challenge. Taken together, these results indicate for the first time that P. fluorescens FHA is a key virulence factor essential to multiple biological processes associated with pathogenicity.

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Mutations of flagellar genesfliC12,fliAandflhDCofEdwardsiella tardaattenuated bacterial motility, biofilm formation and virulence to fish

Cited by 33 publications

References 34 publications

Identification by Tn‐seq of Dickeya dadantii genes required for survival in chicory plants

Identification by Tn‐seq of Dickeya dadantii genes required for survival in chicory plants

Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production

Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity

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