Mutations of t-complex testis expressed gene 5 transcripts in the testis of sterile t-haplotype mutant mouse

Han, Yang; Song, Xue-Xiong; Feng, Haoran; Cheung, Che-Kwok; Lam, P. M.; Wang, Chi Chiu; Haines, C.J.

doi:10.1111/j.1745-7262.2008.00323.x

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…PPP1R11 (Protein Phosphatase 1 Regulatory Subunit 11) was originally described to interact with and inhibit protein phosphatase 1 activity (Zhang et al, 1998) . It also has another name, TcTex5 (T-Complex-Associated-Testis-Expressed 5), and it was implied that mutation of TcTex5 is associated with a mouse sperm motility abnormality in sterile t-haplotype mutant mice (Han et al, 2008; Pilder et al, 2007). However, its E3 ligase activity has never been described.…”

Section: Discussionmentioning

confidence: 99%

RING finger E3 ligase PPP1R11 regulates TLR2 signaling and innate immunity

McKelvey

Dunn

Evankovich

et al. 2016

eLife

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Toll-like receptor 2 (TLR2) is a pattern recognition receptor that recognizes many types of PAMPs that originate from gram-positive bacteria. Here we describe a novel mechanism regulating TLR2 protein expression and subsequent cytokine release through the ubiquitination and degradation of the receptor in response to ligand stimulation. We show a new mechanism in which an uncharacterized RING finger E3 ligase, PPP1R11, directly ubiquitinates TLR2 both in vitro and in vivo, which leads to TLR2 degradation and disruption of the signaling cascade. Lentiviral gene transfer or knockdown of PPP1R11 in mouse lungs significantly affects lung inflammation and the clearance of Staphylococcus aureus. There is a negative correlation between PPP1R11 and TLR2 levels in white blood cell samples isolated from patients with Staphylococcus aureus infections. These results suggest that PPP1R11 plays an important role in regulating innate immunity and gram-positive bacterial clearance by functioning, in part, through the ubiquitination and degradation of TLR2.DOI: http://dx.doi.org/10.7554/eLife.18496.001

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Section: Discussionmentioning

confidence: 99%

RING finger E3 ligase PPP1R11 regulates TLR2 signaling and innate immunity

McKelvey

Dunn

Evankovich

et al. 2016

eLife

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“…For example, the different isoforms of PPP1R11 (also known as TCTEX5) were found to be expressed in most mouse tissues with high expression in testis, epididymis, and in the head and tail regions of spermatozoa (Han et al, 2007). In a subsequent study, it was shown that mutations in the long transcript of PPP1R11 were associated with normal sperm function (Han et al, 2008). The authors concluded that PPP1R11 plays important roles in sperm motility and spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics between fly, mouse, and cattle identifies genes associated with sire conception rate

Peñagaricano

Weigel

et al. 2012

Journal of Dairy Science

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The decline in reproductive performance in cattle is of major concern to farmers and the dairy industry worldwide. Most fertility studies in cattle have focused on fertility of the cow, whereas the genetics of male fertility have not been thoroughly investigated. The present study hypothesizes that the high conservation of spermatogenesis genes from fly to human implies important roles of these genes in male fertility in cattle. To test this hypothesis, we performed an association analysis between highly conserved spermatogenesis genes and sire conception rate (SCR) in US Holsteins as a measure of bull fertility. Sequencing analysis revealed 24 single nucleotide polymorphisms (SNP) in 9 genes in the bull population using the pooled DNA sequencing approach. Five SNP previously identified in 5 genes from the POU1F1 pathway were also included in this study because they have shown significant associations with female and male fertility traits. Overall, 29 SNP located in 14 candidate genes were tested for association with sire conception rate in a population of 1,988 bulls. Three SNP located in MAP1B and 1 SNP in PPP1R11 showed significant associations with SCR. For the POU1F1 pathway, single gene analysis revealed significant associations of POU1F1 and STAT5A with SCR. Analysis of genotypic interactions between adjacent genes in the pathway revealed significant associations of STAT5A and UTMP genotypic combinations with SCR. The most significant spermatogenesis gene, MAP1B, was found to be associated with fertilization and blastocyst rates. Thus, the association of these genes with bull fertility testifies to the usefulness of the comparative genomics approach in selecting candidate male fertility genes.

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“…Cell protein lysates were generated using RIPA Lysis Buffer (Beyotime, China) and were added to PMSF (Beyotime) at a ratio of 100:1. Cellular proteins were extracted 48 h posttransfection, the number of cells was 10 [6]. Proteins were separated by SDS-PAGE and were transferred into polyvinylidene fluoride membranes (Millipore, USA).…”

Section: Western Blot Analysismentioning

confidence: 99%

“…In our previous work, PPP1R11 (protein phosphatase 1 regulatory subunit 11) gene was significantly expressed in pigs after SS2 challenged [3]. The PPP1R11 gene is a member of the PPP (protein phosphatases) superfamily, which encodes protein inhibitor 3 (Inh3) to inhibit PP1, which regulates diverse cellular processes, such as cell cycle, transcription, protein synthesis and metabolism [4][5][6][7]. The role of PPP1R11 in immune response has been paid more attention than in other functions [8,9].…”

Section: Introductionmentioning

confidence: 99%

Transcription elements AREB6 and miR-34a affect apoptosis of PAMs by regulating the expression of SS2-related gene PPP1R11

Liu

Qiao

et al. 2019

Cell Cycle

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In our previous work, gene PPP1R11 (protein phosphatase 1 regulatory subunit 11) was significantly expressed in pigs after Streptococcus suis 2 (SS2) challenged. This study firstly confirmed that SS2 induced significant expression of PPP1R11 gene in porcine alveolar macrophage (PAM) cells, and apoptosis of PAM cells were observed. After that, the core promoter of porcine PPP1R11 was identified and its transcription factor AREB6 which significantly regulated PPP1R11. We also characterized that the PPP1R11 gene is a target of miR-34a. Further, we found that PPP1R11 helped to inhibit apoptosis of PAM cells under SS2 infecting, through transcription factor AREB6 was negatively correlated with apoptosis whereas miR-34a was positively correlated. Those findings provide a functional connection among the transcription factor AREB6, miR-34a, PPP1R11 gene and apoptosis of PAM cells in the pathogenesis of the SS2 infection.

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Mutations of t-complex testis expressed gene 5 transcripts in the testis of sterile t-haplotype mutant mouse

Abstract: We established that mutations that occur in the Tctex5 long transcript of the t-haplotype mice are important for normal sperm function, whereas the short transcript of Tctex5 might have a conserved function among different tissues.

Cited by 6 publications

References 16 publications

RING finger E3 ligase PPP1R11 regulates TLR2 signaling and innate immunity

RING finger E3 ligase PPP1R11 regulates TLR2 signaling and innate immunity

Comparative genomics between fly, mouse, and cattle identifies genes associated with sire conception rate

Transcription elements AREB6 and miR-34a affect apoptosis of PAMs by regulating the expression of SS2-related gene PPP1R11

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