Mutations specific to spore maturation in the asexual fruiting body of dicytostelium discoideum

Williams, Keith L.; Welker, Dennis L.

doi:10.1002/dvg.1020010408

Cited by 8 publications

References 24 publications

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Intracellular cAMP in Dictyostelium discoideum: Characterization of an aggregation‐deficient mutant with elevated cAMP pools

Chan¹,

Coukell²

1981

Dev. Genet.

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Forty aggregation-deficient mutants of Dictyostelium discoideum were screened for changes in intracellular cAMP during the first 10 hr of starvation. The pools in 39 of the mutants remained low and relatively static during this period. However, amoebae of one mutant, strain HC151, exhibited significantly elevated levels of intracellular cAMP during vegetative growth and for several hours after starvation. A more detailed analysis of this mutant indicated that the elevated cAMP pools in these cells are a consequence of the premature appearance and partial activation of an adenylate cyclase. The mutation(s) altering adenylate cyclase regulation in this strain appears to map in linkage group 1V. Complementation tests between strain HC151 and another mutant, HH201, which has recently been shown to produce an adenylate cyclase activity precociously [ 1). indicated that the mutations affecting adenylate cyclase activity in these strains map at different loci. Although both of these mutations behave recessively in heterozygous diploids with respect to gross development, an examination of early cAMP metabolism and terminal spore differentiation in these diploids suggest that these mutations are at least partially expressed during some stage(s) of the developmental cycle.

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Intracellular cAMP in Dictyostelium discoideum: Characterization of an aggregation‐deficient mutant with elevated cAMP pools

Chan¹,

Coukell²

1981

Dev. Genet.

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Genetic and cytological characterisation of fusion chromosomes of Dictyostelium discoideum

Welker

Williams

1981

Chromosoma

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Abnormally large chromosomes which appear to result from the fusion of 2 chromosomes of the normal karyotype have been found in diploids of Dictyostelium discoideum formed by parasexual fusion of haploid strains HU483 (N = 7) and HU245 (n = 7). These fusion chromosomes appear to be the products of the tandem translocation of most, if not all, of one acrocentric chromosome to the telomere of a second acrocentric. Thus the chromosome number of the diploids is reduced from the normal 2n = 14 to 2n = 13 with the formation of an abnormally large acrocentric fusion chromosome. Experimental haploidisation of such diploids result in two types of products, those with a normal 7 chromosome karyotype and those with an abnormal 6 chromosome karyotype which contains the fusion chromosome. Genetic analysis of haploid segregants indicates that linkage groups II and VII are involved in this fusion. Phenotypes of recombinant diploids obtained following mitotic crossing-over establishes that linkage group II is proximal to linkage group VII. Cytological examination of the karyotypes of haploid strains bearing the fusion chromosome suggest that chromosome 2 may correspond to linkage group II and chromosome 3 to linkage group VII. Haploid strains bearing the fusion chromosome grow and develop normally so little or no genetic information can have been lost in the fusion event. While the nature of this event is unknown it may have involved aberrant recombinational DNA repair since the parental haploid strain HU483 bears the radB13 DNA repair mutation.

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Genetics

Newell¹

1982

The Development of Dictyostelium Discoideum

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Mutations specific to spore maturation in the asexual fruiting body of dicytostelium discoideum

Cited by 8 publications

References 24 publications

Intracellular cAMP in Dictyostelium discoideum: Characterization of an aggregation‐deficient mutant with elevated cAMP pools

Intracellular cAMP in Dictyostelium discoideum: Characterization of an aggregation‐deficient mutant with elevated cAMP pools

Genetic and cytological characterisation of fusion chromosomes of Dictyostelium discoideum

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