Mutual antagonism between Mannheimia haemolytica and Pasteurella multocida when forming a biofilm on bovine bronchial epithelial cells in vitro

Boukahil, Ismail; Czuprynski, Charles J.

doi:10.1016/j.vetmic.2018.02.015

Cited by 9 publications

(19 citation statements)

References 29 publications

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“…With its broad host range comprising humans, domestic animals and wild animals [ 1 , 6 , 7 ], P. multocida is considered as one of the most prevalent commensals and opportunistic pathogens worldwide [ 7 , 8 ]. As a commensal of cattle, P. multocida is located in the upper respiratory tract [ 1 , 9 – 11 ]; the pathogenesis of the respiratory disease and the interactions with other respiratory pathogens are largely unknown. Several bacterial components have been identified as virulence factors, e.g.…”

Section: Introductionmentioning

confidence: 99%

Infection of bovine well-differentiated airway epithelial cells by Pasteurella multocida: actions and counteractions in the bacteria–host interactions

Tong

et al. 2020

Vet Res

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Pasteurella (P.) multocida is a zoonotic pathogen, which is able to cause respiratory disorder in different hosts. In cattle, P. multocida is an important microorganism involved in the bovine respiratory disease complex (BRDC) with a huge economic impact. We applied air–liquid interface (ALI) cultures of well-differentiated bovine airway epithelial cells to analyze the interaction of P. multocida with its host target cells. The bacterial pathogen grew readily on the ALI cultures. Infection resulted in a substantial loss of ciliated cells. Nevertheless, the epithelial cell layer maintained its barrier function as indicated by the transepithelial electrical resistance and the inability of dextran to get from the apical to the basolateral compartment via the paracellular route. Analysis by confocal immunofluorescence microscopy confirmed the intactness of the epithelial cell layer though it was not as thick as the uninfected control cells. Finally, we chose the bacterial neuraminidase to show that our infection model is a sustainable tool to analyze virulence factors of P. multocida. Furthermore, we provide an explanation, why this microorganism usually is a commensal and becomes pathogenic only in combination with other factors such as co-infecting microorganisms.

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Section: Introductionmentioning

confidence: 99%

Infection of bovine well-differentiated airway epithelial cells by Pasteurella multocida: actions and counteractions in the bacteria–host interactions

Tong

et al. 2020

Vet Res

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“…In healthy cattle, M. haemolytica is a natural inhabitant of the upper respiratory tract including the nasal passages, nasopharynx, and tonsils; paranasal sinuses are predominately sterile, and M. haemolytica was isolated from transtracheal fluids from 13.1% of healthy cattle (Frank and Briggs, 1992; Frank et al ., 1995; Murray et al ., 2017; Timsit et al ., 2017). M. haemolytica live within biofilms on the upper respiratory mucosa (Olson et al ., 2002; Boukahil and Czuprynski, 2015, 2016, 2018). Multiple surface adhesins, including several surface proteins, fimbriae, and the polysaccharide capsule, are responsible for adherence of M. haemolytica to the upper respiratory mucosa and colonization (Morck et al ., 1988; Jaramillo et al ., 2000; Lo, 2001; Gioia et al ., 2006; Daigneault and Lo, 2009; Kisiela and Czuprynski, 2009).…”

Section: Introductionmentioning

confidence: 99%

Mannheimia haemolyticain bovine respiratory disease: immunogens, potential immunogens, and vaccines

Confer

Ayalew

2018

Anim. Health. Res. Rev.

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Mannheimia haemolytica is the major cause of severe pneumonia in bovine respiratory disease (BRD). Early M. haemolytica bacterins were either ineffective or even enhanced disease in vaccinated cattle, which led to studies of the bacterium's virulence factors and potential immunogens to determine ways to improve vaccines. Studies have focused on the capsule, lipopolysaccharide, various adhesins, extracellular enzymes, outer membrane proteins, and leukotoxin (LKT) resulting in a strong database for understanding immune responses to the bacterium and production of more efficacious vaccines. The importance of immunity to LKT and to surface antigens in stimulating immunity led to studies of individual native or recombinant antigens, bacterial extracts, live-attenuated or mutant organisms, culture supernatants, combined bacterin-toxoids, outer membrane vesicles, and bacterial ghosts. Efficacy of several of these potential vaccines can be shown following experimental M. haemolytica challenge; however, efficacy in field trials is harder to determine due to the complexity of factors and etiologic agents involved in naturally occurring BRD. Studies of potential vaccines have led current commercial vaccines, which are composed primarily of culture supernatant, bacterin-toxoid, or live mutant bacteria. Several of those can be augmented experimentally by addition of recombinant LKT or outer membrane proteins.

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“…Another H. somni ICE, ICEHs1, was confirmed to confer tetracycline resistance and copper and zinc tolerance to recipient strains (Bhatt et al 2018). The three BRD pathogens H. somni, P. multocida, and M. haemolytica are members of the Pasteurellaceae family and are known to live together in biofilms in the respiratory tract of cattle (Boukahil and Czuprynski 2018;Petruzzi et al 2020). Inhabiting biofilms enables physical interactions between microbial community members and facilitates horizontal gene transfer (HGT) between bacteria (Bañuelos et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Examination of the horizontal gene transfer dynamics of an integrative and conjugative element encoding multidrug resistance in Histophilus somni

et al. 2023

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Integrative and conjugative elements (ICEs) are self-transferable mobile genetic elements that play a significant role in disseminating antimicrobial resistance between bacteria via horizontal gene transfer (HGT). A recently identified ICE in a clinical isolate of Histophilus somni (ICEHs02) is 72,914 base-pairs in length and harbours seven predicted antimicrobial resistance genes (ARG) conferring resistance to tetracycline (tetR-tet(H)), florfenicol (floR), sulfonamide (Sul2), aminoglycoside (APH(3'')-Ib), (APH(6)-Id), (APH(3')-Ia), and copper (mco). This study investigated ICEHs02 host range, assessed effects of antimicrobial stressors on transfer frequency, and examined effects of ICEHs02 acquisition on hosts. Conjugation assays examined transfer frequency of ICEHs02 to Histophilus somni and Pasteurella multocida strains. PCR assays confirmed the presence of a circular intermediate, ICE-associated core genes, and cargo genes in recipient strains. Susceptibility testing examined ICEHs02-associated resistance phenotypes in recipient strains. Tetracycline and ciprofloxacin induction significantly increased the transfer rates of ICEHs02 in vitro. The copy numbers of the circular intermediate of ICEHs02 per chromosome exhibited significant increases of ~ 37-fold after tetracycline exposure, and ~ 4-fold after ciprofloxacin treatment. Acquisition of ICEHs02 reduced relative fitness of H. somni transconjugants by 28% (w = 0.72 ± 0.04) and P. multocida TG relative fitness was decreased 15% (w = 0.85 ± 0.01).

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Mutual antagonism between Mannheimia haemolytica and Pasteurella multocida when forming a biofilm on bovine bronchial epithelial cells in vitro

Cited by 9 publications

References 29 publications

Infection of bovine well-differentiated airway epithelial cells by Pasteurella multocida: actions and counteractions in the bacteria–host interactions

Infection of bovine well-differentiated airway epithelial cells by Pasteurella multocida: actions and counteractions in the bacteria–host interactions

Mannheimia haemolyticain bovine respiratory disease: immunogens, potential immunogens, and vaccines

Examination of the horizontal gene transfer dynamics of an integrative and conjugative element encoding multidrug resistance in Histophilus somni

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