MWCNTs enhance hBMSCs spreading but delay their proliferation in the direction of differentiation acceleration

Deligianni, Despina

doi:10.4161/19336918.2014.969999

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…Contrarily to the PLGA, the use of MWCNTs is controversy with their biocompatibility. However, due to their nanometer scale and extraordinary physicochemical properties, supporting the proliferation of different kinds of cells, MWCNTs have won popularity and have been used in tissue engineering applications, including in bone tissue . By analyzing Figure , it is observed that just neat PLGA and the PLGA/MWCNTs composite with 0.1% of MWCNTs are no cytotoxic (cell viability reduction is less than 30%).…”

Section: Resultsmentioning

confidence: 99%

Hydrolytic degradation and cytotoxicity of poly(lactic‐co‐glycolic acid)/multiwalled carbon nanotubes for bone regeneration

Dı́az

Puerto

Sandonis

et al. 2019

J of Applied Polymer Sci

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Biodegradable poly(L-lactide-co-glycolide) (PLGA)/multiwalled carbon nanotubes (MWCNTs) scaffolds produced by thermally induced phase separation (TIPS) are studied for bone regeneration. Their magnetic properties, cytotoxicity, and in vitro degradation are investigated. Certain properties are analyzed at 37 C over 16 weeks in phosphate buffer saline (PBS) solution, as a function of degradation time: morphology, mass loss, pH value of PBS, and thermal behavior. The presence of small quantities of nanotubes in the scaffolds, ≤0.5 wt %, leads to a weak magnetic response although the PLGA was diamagnetic. The incorporation of MWCNTs in the scaffolds generated a morphology and a very different process of in vitro degradation than might be expected in a PLGA scaffold. The in vitro degradation process started on week 13 and rapidly advanced, although the structural integrity of the scaffolds was maintained and no collapse of the structure occurred. Cytotoxicity tests on the samples showed cytotoxicity behavior at concentrations of over 0.3 wt % MWCNTs.

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Section: Resultsmentioning

confidence: 99%

Hydrolytic degradation and cytotoxicity of poly(lactic‐co‐glycolic acid)/multiwalled carbon nanotubes for bone regeneration

Dı́az

Puerto

Sandonis

et al. 2019

J of Applied Polymer Sci

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“…Cellular interaction with the substrate and neighboring cells is known to play a critical role in survival, proliferation, and differentiation. 60 Fibroblast cells are anchoragedependent and therefore they require a surface to attach to and then proliferate. In that context, the results showed that fibroblast cell proliferation was delayed when they were cultured in CEL2 bioactive glass-ceramic scaffolds (with or without acetaminophen), through an unknown mechanism, because their proliferation was lower than that of the control cells.…”

Section: Biologic Testsmentioning

confidence: 99%

Preparation of CEL2 glass‐ceramic porous scaffolds coated with chitosan microspheres that have a drug delivery function

Villicaña-Molina

Aguilar-Reyes

León‐Patiño

et al. 2019

Int J Applied Ceramic Tech

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The present work focused on the preparation of CEL2 bioactive glass (SiO2–P2O5–CaO–MgO–K2O–Na2O) scaffolds loaded with chitosan microspheres. Chitosan microspheres, with a mean particle size of 0.55 μm ± 0.25 μm and loaded with acetaminophen, were obtained through the water‐in‐oil single emulsion solvent evaporation method and were adhered to the surface of the scaffolds by a simple dip‐coating technique. The characterization of the microsphere‐loaded scaffolds, before and after immersion in simulated body fluid (SBF), was performed by scanning electron microscopy, X‐ray diffraction, and infrared spectroscopy. In vitro bioactivity was performed for 21 days. The glass‐ceramic microsphere‐loaded scaffolds showed more than 70% interconnected porosity and an average compressive strength of 1.2 ± 0.43 MPa after immersion in SBF. They also showed the formation of a hydroxyapatite layer from the first day of immersion in SBF, demonstrating their high bioactivity. The microspheres were shown to be homogeneously dispersed on the scaffold surfaces. After 120 hours, the biologic tests showed good fibroblast cell proliferation onto the scaffolds. The encapsulated drug in the microspheres was released by diffusion in a sustained manner (90% and 99% in 200 hours). The results suggest that scaffolds have a promising role in applications of bone tissue engineering.

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“…Purified pristine multiwalled carbon nanotubes (MWCNTs) have been found to support adhesion and proliferation of hMSCs, enhance mature osteogenic gene expression, and accelerate cell differentiation, even in the absence of osteogenic supplements. 8,9 Controlling thickness, roughness, and directional alignment of single-walled CNT films would regulate the growth and differentiation of stem cells. 10 On arrays of vertically aligned MWCNTs (VACNTs), unlike cell growth on nonpatterned surfaces, the osteoblast-like cell attachment and spreading process on VACNT was significantly enhanced.…”

Section: Introductionmentioning

confidence: 99%

Carbon Nanotube Surface Regular Topography Improves Cell Response, Depending on Cell Passage

Kroustalli

Deligianni

2016

Bone�Tissue�Regen�Insights

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Through mediation of integrin clustering, nanoscale surface structure of carbon nanotubes (CNTs) directly affects cell binding and subsequent behavior. The influence of morphological structures on proliferation, differentiation, and organization of focal adhesions and cytoskeleton of human mesenchymal stem cells (hMSCs) was studied. The following two surface morphologies were fabricated and examined: a random network multiwalled carbon nanotubes film (RNCNT) and a vertically aligned multiwalled carbon nanotube (VACNT) film. hMSCs adhered and spread earlier on both CNT surfaces than on control. A statistically significantly increased number of attached cells were observed on VACNT surfaces. No CNT substrate enhanced differentiation, but both maintained the differentiation property of hMSCs. VACNTs recruited vinculin from the cytoplasm for the formation of focal adhesion complexes earlier in comparison to RNCNT. There is still disparity on how nanostructures regulate the progression toward an osteoblastic phenotype. It is necessary to explore various architectures in order to understand how they initiate osteoinductive or proliferating signals.

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MWCNTs enhance hBMSCs spreading but delay their proliferation in the direction of differentiation acceleration

Cited by 6 publications

References 29 publications

Hydrolytic degradation and cytotoxicity of poly(lactic‐co‐glycolic acid)/multiwalled carbon nanotubes for bone regeneration

Hydrolytic degradation and cytotoxicity of poly(lactic‐co‐glycolic acid)/multiwalled carbon nanotubes for bone regeneration

Preparation of CEL2 glass‐ceramic porous scaffolds coated with chitosan microspheres that have a drug delivery function

Carbon Nanotube Surface Regular Topography Improves Cell Response, Depending on Cell Passage

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