Summer rice cannot be grown near the coast of the Ayeyarwady Delta, Myanmar, because of the high salinity in river water during the dry season. This means that saline intrusion should be monitored to manage the irrigated rice-growing area. The aim of this study therefore is to develop a model to estimate the salinity of river water using satellite imagery. The imagery of Sentinel-2 was suitable for monitoring saline intrusion because of their high spatial (10-m) and temporal (10-day) resolutions. We found that the reflectance of the visible bands was correlated with electrical conductivity, which was influenced by the concentration and composition of dissolved salts. When the river water mixed with the salt water from the sea, suspended particles tended to flocculate and settle, as a result, less turbid water was more saline. The best-fitting model was obtained with the green band (coefficient of determination R2 of 0.776, root mean square error of 4.896 dSm−1, and mean absolute error of 3.340 dS m−1). The saline intrusion showed considerable spatial and temporal variability during the dry season. The salinity intrusion extended approximately 80 km inland at the end of the dry season in March in the Pathein River. The 1 ppt salt concentration line in March marked the boundary between cultivated and non-cultivated areas of paddy field, which indicates that cultivable areas were strongly affected by saline intrusion. The results show that more frequent and higher resolution monitoring than before of the Sentinel-2 can support effective water resource management.