MYB75 Phosphorylation by MPK4 Is Required for Light-Induced Anthocyanin Accumulation in Arabidopsis

Li, Shengnan; Wang, Wenyi; Gao, Jinlan; Yin, Kangquan; Wang, Rui; Wang, Yuming; Petersen, Morten; Mundy, John; Qiu, Jin‐Long

doi:10.1105/tpc.16.00130

Cited by 184 publications

(129 citation statements)

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“…Over‐expression of MYB75 results in accumulation of anthocyanin and protects A . thaliana from high light intensity (Gonzalez, Zhao, Leavitt, & Lloyd, ; Li et al, ). Glucosinolates‐myrosinase system genes such as flavin monooxygenase glucosinolate s‐oxygenase 2, alkenyl hydroxyalkyl producing 2, MYB domain protein 76, myrosinase binding protein 1, and epithiospecifier protein were up‐regulated in At MIOX4 OE line compared to WT (Figure a).…”

Section: Discussionmentioning

confidence: 99%

“…Phenylpropanoid biosynthesis genes (TSM1, CAD-B2), flavonoid biosynthesis (MYB75), the JAO2, JAO3, JAO4, AACT1 genes, and terpene biosynthesis genes (GGPS6, LAS1, TPS10) were up-regulated in the high AsA line compared to controls (Figure 11a). Over-expression of MYB75 results in accumulation of anthocyanin and protects A. thaliana from high light intensity (Gonzalez, Zhao, Leavitt, & Lloyd, 2008;Li et al, 2016). Glucosinolates-myrosinase system genes such as flavin monooxygenase glucosinolate s-oxygenase 2, alkenyl hydroxyalkyl producing 2, MYB domain protein 76, myrosinase binding protein 1, and epithiospecifier protein were up-regulated in AtMIOX4 OE line compared to WT (Figure 12a).…”

Section: High Ascorbate Plants Are Transcriptionally Primed For Hermentioning

confidence: 99%

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Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

et al. 2019

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Myo ‐inositol oxygenase (MIOX) is the first enzyme in the inositol route to ascorbate (L‐ascorbic acid, AsA, vitamin C). We have previously shown that Arabidopsis plants constitutively expressing MIOX have elevated foliar AsA content and displayed enhanced growth rate, biomass accumulation, and increased tolerance to multiple abiotic stresses. In this work, we used a combination of transcriptomics, chromatography, microscopy, and physiological measurements to gain a deeper understanding of the underlying mechanisms mediating the phenotype of the At MIOX4 line. Transcriptomic analysis revealed increased expression of genes involved in auxin synthesis, hydrolysis, transport, and metabolism, which are supported by elevated auxin levels both in vitro and in vivo, and confirmed by assays demonstrating their effect on epidermal cell elongation in the At MIOX4 over‐expressers. Additionally, we detected up‐regulation of transcripts involved in photosynthesis and this was validated by increased efficiency of the photosystem II and proton motive force. We also found increased expression of amylase leading to higher intracellular glucose levels. Multiple gene families conferring plants tolerance/expressed in response to cold, water limitation, and heat stresses were found to be elevated in the At MIOX4 line. Interestingly, the high AsA plants also displayed up‐regulation of transcripts and hormones involved in defense including jasmonates, defensin, glucosinolates, and transcription factors that are known to be important for biotic stress tolerance. These results overall indicate that elevated levels of auxin and glucose, and enhanced photosynthetic efficiency in combination with up‐regulation of abiotic stresses response genes underly the higher growth rate and abiotic stresses tolerance phenotype of the At MIOX4 over‐expressers.

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Section: Discussionmentioning

confidence: 99%

Section: High Ascorbate Plants Are Transcriptionally Primed For Hermentioning

confidence: 99%

Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

et al. 2019

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“…Anthocyanin accumulated specifically in cells in which PAP1 was expressed, although it took several days for strong visible pigmentation to develop. We used the wild‐type coding sequence for PAP1, but as PAP1 requires phosphorylation for stability and full activity, anthocyanin accumulation could be accelerated by use of phospho‐mimic mutations (Li et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Inducible reporter/driver lines for the Arabidopsis root with intrinsic reporting of activity state

et al. 2019

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Cell-, tissue-or organ-specific inducible expression systems are powerful tools for functional analysis of changes to the pattern, level or timing of gene expression. However, plant researchers lack standardised reagents that promote reproducibility across the community. Here, we report the development and functional testing of a Gateway-based system for quantitatively, spatially and temporally controlling inducible gene expression in Arabidopsis that overcomes several drawbacks of the legacy systems. We used this modular driver/effector system with intrinsic reporting of spatio-temporal promoter activity to generate 18 well-characterised homozygous transformed lines showing the expected expression patterns specific for the major cell types of the Arabidopsis root; seed and plasmid vectors are available through the Arabidopsis stock centre. The system's tight regulation was validated by assessing the effects of diphtheria toxin A chain expression. We assessed the utility of Production of Anthocyanin Pigment 1 (PAP1) as an encoded effector mediating cell-autonomous marks. With this shared resource of characterised reference driver lines, which can be expanded with additional promoters and the use of other fluorescent proteins, we aim to contribute towards enhancing reproducibility of qualitative and quantitative analyses.

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“…Phenylpropanoid biosynthesis genes ( TSM1, CAD-B2 ), flavonoid biosynthesis ( MYB75 ), the JAO2, JAO3, JAO4, AACT1 genes and terpene biosynthesis genes ( GGPS6, LAS1, TPS10 ) were up-regulated in the high AsA line compared to controls (Figure 11A). Over-expression of MYB75 results in accumulation of anthocyanin and protects A. thaliana from high light intensity (Gonzalez et al, 2008; Li et al, 2016). Glucosinolates-myrosinase system genes such as flavin monoxygenase glucosinolate s-oxygenase 2, alkenyl hydroxalkyl producing 2, MYB domain protein 76, myrosinase binding protein 1, and epithiospecifier protein were up-regulated in At MIOX4 OE line compared to WT (Figure 12A).…”

Section: Discussionmentioning

confidence: 99%

Mechanisms Underlying the Enhanced Biomass and Abiotic Stress Tolerance Phenotypes of an Arabidopsis MIOX Over-expresser

Nepal

Yactayo-Chang

Medina‐Jiménez

et al. 2019

Preprint

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1Myo-inositol oxygenase (MIOX) is the first enzyme in the inositol route to ascorbate (L-2 ascorbic acid, AsA, vitamin C). We have previously shown that Arabidopsis plants 3 constitutively expressing MIOX have elevated foliar AsA content and displayed enhanced 4 growth rate, biomass accumulation, and increased tolerance to multiple abiotic stresses. 5In this work, we used a combination of transcriptomics, chromatography, microscopy, 6 and physiological measurements to gain a deeper understanding of the underlying 7 mechanisms mediating the phenotype of the AtMIOX4 line. Transcritpomic analysis 8 revealed increased expression of genes involved in auxin synthesis, hydrolysis, transport, 9 and metabolism, which are supported by elevated auxin levels both in vitro and in vivo, 10 and confirmed by assays demonstrating their effect on epidermal cell elongation in the 11AtMIOX4 over-expresser plants. Additionally, we detected up-regulation of transcripts 12 involved in photosynthesis that was validated by increased efficiency of the photosystem 13 II and proton motive force. We also found increased expression of amylase leading to 14 higher intracellular glucose levels. Multiple gene families conferring plants tolerance to 15 cold, water limitation, and heat stresses were found to be elevated in the AtMIOX4 line. 16Interestingly, the high AsA plants also displayed up-regulation of transcripts and 17 hormones involved in defense including jasmonates, defensin, glucosinolates, and 18 transcription factors that are known to be important for biotic stress tolerance. These 19 results overall indicate that elevated levels of auxin and glucose, and enhanced 20 photosynthetic efficiency in combination with up-regulation of abiotic stresses response 21 genes underly the higher growth rate and abiotic stresses tolerance phenotype of the 22 AtMIOX4 over-expressers. 23 (Keywords: ascorbate, ascorbic acid, vitamin C, abiotic stress, redox biology) 24 25 26 27 28 29 30 31 related (PR) genes via reduced glutathione accumulation (Pavet et al., 2006).

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MYB75 Phosphorylation by MPK4 Is Required for Light-Induced Anthocyanin Accumulation in Arabidopsis

Cited by 184 publications

References 72 publications

Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

Mechanisms underlying the enhanced biomass and abiotic stress tolerance phenotype of an Arabidopsis MIOX over‐expresser

Inducible reporter/driver lines for the Arabidopsis root with intrinsic reporting of activity state

Mechanisms Underlying the Enhanced Biomass and Abiotic Stress Tolerance Phenotypes of an Arabidopsis MIOX Over-expresser

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