MYC in Brain Development and Cancer

Zaytseva, Olga; Kim, Nan‐Hee; Quinn, Leonie M.

doi:10.3390/ijms21207742

Cited by 25 publications

(23 citation statements)

References 99 publications

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“…[ 38 ] It has been reported that inhibition of this protein could result in the suppression of tumor growth. [ 39 ] Given that SadM‐CBE had a higher efficiency in the PAM‐proximal regions ( Figure 8 A ), it was a promising tool to introduce a stop codon in MYC via base editing for oncogene disruption. To further verify the superiority of SadM‐CBE for the editing of PAM‐proximal cytosines, the hyBE4max including a DNA binding protein RAD51 between APOBEC1 and Cas9 that was reported to have a broader editing window [ 40 ] was chosen for the comparison for the PAM‐proximal editing at six genomic loci.…”

Section: Resultsmentioning

confidence: 99%

Pioneer Factor Improves CRISPR‐Based C‐To‐G and C‐To‐T Base Editing

Dong

et al. 2022

Advanced Science

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Base editing events in eukaryote require a compatible chromatin environment, but there is little research on how chromatin factors contribute to the editing efficiency or window. By engineering BEs (base editors) fused with various pioneer factors, the authors found that SOX2 substantially increased the editing efficiency for GBE and CBE. While SoxN‐GBE (SOX2‐NH3‐GBE) improved the editing efficiency at overall cytosines of the protospacer, SoxM‐GBE/CBE (SOX2‐Middle‐GBE/CBE) enabled the higher base editing at PAM‐proximal cytosines. By separating functional domains of SOX2, the SadN‐GBE (SOX2 activation domain‐NH3‐GBE) is constructed for higher editing efficiency and SadM‐CBE for broader editing window to date. With the DNase I assay, it is also proved the increased editing efficiency is most likely associated with the induction of chromatin accessibility by SAD. Finally, SadM‐CBE is employed to introduce a stop codon in the proto‐oncogene MYC, at a locus rarely edited by previous editors with high efficiency. In this work, a new class of pioneer‐BEs is constructed by fusion of pioneer factor or its functional domains, which exhibits higher editing efficiency or broader editing window in eukaryote.

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Section: Resultsmentioning

confidence: 99%

Pioneer Factor Improves CRISPR‐Based C‐To‐G and C‐To‐T Base Editing

Dong

et al. 2022

Advanced Science

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“…MYC is a regulator of gene transcription and controls a diverse set of biological programs (28,29). MYC can promote programs of proliferative cell growth; thus, MYC is frequently up-regulated in tumors (30). MYC is associated with many cancers' progression (31).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of fatty acid and lactate metabolism–related genes for prognosis value, immune infiltration, and therapy in osteosarcoma patients

Han²,

Su³

et al. 2022

Front. Oncol.

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Osteosarcoma is the most frequent bone tumor. Notwithstanding that significant medical progress has been achieved in recent years, the 5-year overall survival of osteosarcoma patients is inferior. Regulation of fatty acids and lactate plays an essential role in cancer metabolism. Therefore, our study aimed to comprehensively assess the fatty acid and lactate metabolism pattern and construct a fatty acid and lactate metabolism–related risk score system to predict prognosis in osteosarcoma patients. Clinical data and RNA expression data were downloaded from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) and Gene Expression Omnibus (GEO) databases. We used the least absolute shrinkage and selection operator (LASSO) and Cox regression analyses to construct a prognostic risk score model. Relationships between the risk score model and age, gender, tumor microenvironment characteristics, and drug sensitivity were also explored by correlation analysis. We determined the expression levels of prognostic genes in osteosarcoma cells via Western blotting. We developed an unknown fatty acid and lactate metabolism–related risk score system based on three fatty acid and lactate metabolism–related genes (SLC7A7, MYC, and ACSS2). Survival analysis showed that osteosarcoma patients in the low-risk group were likely to have a better survival time than those in the high-risk group. The area under the curve (AUC) value shows that our risk score model performs well in predicting prognosis. Elevated fatty acids and lactate risk scores weaken immune function and the environment of the body, which causes osteosarcoma patients’ poor survival outcomes. In general, the constructed fatty acid and lactate metabolism–related risk score model can offer essential insights into subsequent mechanisms in available research. In addition, our study may provide rational treatment strategies for clinicians based on immune correlation analysis and drug sensitivity in the future.

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“…In turn, activation of p21 and p27 can occur using p53 and p73, for which L1 inhibition is also shown [321]. MYC proto-oncogene is involved in many cell processes and in the regulation of the cell cycle, the defects of which are often found in cancer [384]. MYC binding to the L1 5 UTR represses transcriptional activity.…”

Section: Regulation Of L1 By Cell Cycle Factorsmentioning

confidence: 99%

Factors Regulating the Activity of LINE1 Retrotransposons

Protasova

Andreeva

Rogaev

2021

Genes

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LINE-1 (L1) is a class of autonomous mobile genetic elements that form somatic mosaicisms in various tissues of the organism. The activity of L1 retrotransposons is strictly controlled by many factors in somatic and germ cells at all stages of ontogenesis. Alteration of L1 activity was noted in a number of diseases: in neuropsychiatric and autoimmune diseases, as well as in various forms of cancer. Altered activity of L1 retrotransposons for some pathologies is associated with epigenetic changes and defects in the genes involved in their repression. This review discusses the molecular genetic mechanisms of the retrotransposition and regulation of the activity of L1 elements. The contribution of various factors controlling the expression and distribution of L1 elements in the genome occurs at all stages of the retrotransposition. The regulation of L1 elements at the transcriptional, post-transcriptional and integration into the genome stages is described in detail. Finally, this review also focuses on the evolutionary aspects of L1 accumulation and their interplay with the host regulation system.

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MYC in Brain Development and Cancer

Cited by 25 publications

References 99 publications

Pioneer Factor Improves CRISPR‐Based C‐To‐G and C‐To‐T Base Editing

Pioneer Factor Improves CRISPR‐Based C‐To‐G and C‐To‐T Base Editing

Comprehensive analysis of fatty acid and lactate metabolism–related genes for prognosis value, immune infiltration, and therapy in osteosarcoma patients

Factors Regulating the Activity of LINE1 Retrotransposons

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