Mycelia Biomass Yield of <i>Ganoderma lucidum</i> Mushroom by submerged culture

Abstract

Biomass yield of G. lucidum in submerged culture was investigated on account of its requirements for sources of carbon, nitrogen and the carbon: nitrogen (C:N) ratio. The study was carried out with the culture medium of potato dextrose broth enriched with the different carbon (glucose, maltose, starch) and nitrogen (aspartic acid, nitrogen nitrate, peptone) sources over a period of 15 days following standard procedures. The highest biomass of 460±89mg was recorded in the broth medium incorporated with maltose which is significantly different (p<0.05) from that of glucose, 310±89mg, and lactose, 250±50mg. There was no growth on starch (0.00±0.000mg) while the control recorded 114±20mg. The highest biomass of 200±39mg among the nitrogen sources was recorded in the medium incorporated with aspartic acid which is also significantly different(p<0.05) from that of peptone, nitrogen nitrate and the control. A carbon: nitrogen (C:N) ratio (i.e. maltose : aspartic acid) source of 2:2 supported the highest biomass when compared to other carbon : nitrogen ratios. It was therefore deduced, that the presence of maltose as a source of carbon and aspartic acid as a source of nitrogen respectively favoured a good growth of G. lucidum when supplied in a definite amount and ratio. The implications and reasons for these results were highlighted and discussed. It was concluded therefore, that various carbon and nitrogen sources and certain carbon: nitrogen (C:N) ratio have effect on the biomass yield of G. lucidum grown in Potato dextrose broth medium. The findings of this study will be an addition to the baseline information for the growth of the mushroom in submerged cultures for its pharmacological and medicinal potentials.

Optimization of Culture Conditions for Biomass Production of Ganoderma lucidum

“…Figure 3a, shows that among the six nitrogen sources that were tested, yeast extract was found to be most suitable for the growth of mycelial biomass (279±2.18 mg/mL), followed by peptone, which yielded a dry mycelial biomass of 259±3.33 mg/100mL. Hence, organic nitrogen sources were found to be better than the inorganic nitrogen sources, which are in accordance to the study done previously on G. lucidum (Suberu et al, 2013).…”

“…In a study done by (Suberu et al, 2013) on the growth of G. lucidum mycelia, maltose gave the highest yield followed by glucose. Many studies done on other basidiomycetes like G. frondosa and Psathyerella atroumbonata, showed glucose to be the most effective carbon source for mycelial biomass production (Lee et al, 2004;Jonathan and Fasidi, 2003).…”

Growth performance of Ganoderma lucidum using billet method in Garhwal Himalaya, India

“…For the experiment, the pure culture of G. lucidum was prepared from its fruiting body. The fruiting body was incised into small pieces and was inoculated in Potato Dextrose Agar (PDA) medium in a Petri-dish at room temperature (25 ± 3 °C) ( Ayodele et al, 2007 , Suberu et al, 2013 ). The mycelia of G. lucidum took 7–8 days to properly colonize on the PDA medium and were ready for the preparation of spawn.…”

Statistical optimization of culture conditions for enhanced mycelial biomass production using Ganoderma lucidum

“…The growth of mycelia has been found to be related with various environmental factors such as pH and temperature and the nutrients that are available to it. In general, culture conditions are optimized using a one factor-at-a-time approach, i.e., varying one factor while keeping all the others constant [4][5][6]. However, this method does not allow testing two factors simultaneously which when interacted together could improve the production.…”