Mycelial growth of strains of the genera Suillus and Boletinus in media with a wide range of concentrations of carbon and nitrogen sources

Abstract

Suillus and Boletinus were studied using Ohta medium. In media with glucose or trehalose, all tested strains grew well. With mannose and cellobiose, strains generally grew well, except for one strain of Suillus. Utilization of dextrin and soluble starch differed with each strain, and that of sucrose and glycerol was low for all strains. Utilization of four amino acids, arginine, glutamic acid, aspartic acid, and alanine, was similar to that of ammonium tartrate for Suillus strains, but mycelial growth with amino acids was clearly lower than with ammonium tartrate for the Boletinus strain. The effect of glucose and ammonium tartrate concentrations for nine strains of the genera Suillus and Boletinus was studied with ranges for glucose of 1-100 and 200 g/l, respectively, and for ammonium tartrate of 0.2-5 and 20 g/l, respectively. Six strains showed maximal growth at a glucose concentration greater than 25 g/l, and one strain showed maximal growth at 70 g/l. The results indicate that these fungi are adapted to relatively high concentrations of carbon sources. In general, glucose concentration at mycelial growth maximum decreased as ammonium tartrate concentration increased, and at higher concentrations of glucose, mycelial growth decreased more rapidly in higher concentrations of ammonium tartrate.

Favourable culture conditions for mycelial growth of <i>Hydnum repandum</i>, a medicinal mushroom

“…This result was parallel with the suggestion of Hatakeyama and Ohmasa (2004) who found that the mycelial growth of Suillus and Boletinus was poor in sucrose contained medium. 1 Mycelial growth area and mycelial colony diameter were measured when it was completed in any of Petri plates at the end of the 11th day after mycelium inoculation for the current study; **Means followed by different letters in the same columns are statistically different according to Duncan's multiple range test (P<0.01).…”

EFECTO DEL MEDIO DE CULTIVO EN EL DESARROLLO DE Suillus granulatus (L.) Roussel y S. brevipes (Pk.) Kuntze

“…Therefore, only glucose and some other monosaccharides and disaccharides can be used for growth (Kusuda et al, 2007). It is possible that the low glucose level in the MMN medium (10 g·liter -1 ) was the reason the mycelium grew less, because, as reported by Hatakeyama and Ohmasa (2004a) El PDA fue el mejor medio de cultivo. Este resultado es conveniente para la selección y el aislamiento de las cepas pues es un medio fácil de preparar.…”

“…El estudio del comportamiento y requerimientos de estos microorganismos en cultivo puro, constituye la base de la producción de inóculo; etapa necesaria para la micorrización controlada en plantas de interés forestal y posterior producción de esporomas (Díaz, Flores, & Honrubia, 2009a;Hatakeyama & Ohmasa, 2004a).…”

“…The study of the behavior and requirements of these microorganisms in pure culture is the basis for the production of inoculum, which is a necessary step for controlled mycorrhization in plants of forest interest and subsequent production of sporocarps (Díaz, Flores, & Honrubia, 2009a;Hatakeyama & Ohmasa, 2004a).…”

Growth promotion of Suillus luteus by adenosine in vitro

“…(2) It is well known that adenosine is a precursor of cAMP, and the higher concentration improves the activity of cAMP-dependent protein kinase; however, the enzyme could suppress mycelial growth (Lubbehusen et al 2004). This effect was also found in other carbon and nitrogen components (Hatakeyama and Ohmasa 2004b). Kusuda et al (2007) reported catabolite suppression by higher concentrations of extra glucose in the ectomycorrhizal fungus Tricholoma matsutake.…”