Mycelial incompatibility operative in pairings between single basidiospore isolates of Helicobasidium mompa

Ikeda, Kenichi; Nakamura, Hitoshi; Matsumoto, Naoyuki

doi:10.1017/s0953756203008049

Cited by 18 publications

(9 citation statements)

References 30 publications

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“…The pairs from NS non-significant, S significant * (days after inoculation) ** (Weight of fresh mushroom in 3-harvests/weight of dry substrate) 9 100 World J Microbiol Biotechnol (2011) 27:1-9 5 P. eous and P. florida are not included in the further analysis due to the absence of hyphal fusion (barrage formation) between the isolates. It may be the consequence of either the similar mating type of all the isolates or inability of the isolates to fuse with each other under laboratory conditions (Ikeda et al 2002 Four isolates showed compatibility with H23 and one isolate was compatible with H6 (data not shown). All the other isolates were not compatible with the either of the tester isolate (H6 and H23).…”

Section: Identification Of Mating Types Of Single Basidiosporesmentioning

confidence: 96%

Molecular characterization and mating type analysis of oyster mushroom (Pleurotus spp.) using single basidiospores for strain improvement

Gupta

Reddy

Kotasthane

2010

World J Microbiol Biotechnol

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Existence of variability in morphological traits and growth rate of mycelium of homokaryotic single basidiospores can be exploited for the development of interstrainal hybrids. We isolated 182 single basidiospores from mushroom bodies of P. sajor-caju, P. florida, P. eous and one wild relative of Pleurotus called Hypsizygus ulmaris. The single spores were isolated using a new technique that is less prone to contamination and more efficient than the common techniques used by earlier workers. All the isolates showed a varied range of cultural morphology. Mating types of all the isolates within the species were identified on the basis of hyphal fusion via anastomosis with the tester strains. Four compatible pairs of isolates with well prominent tuft in the contact zone were selected for dikaryon isolation. Dikaryons were used for spawn preparation and mushroom cultivation. The dikaryotic isolates with their replicates were evaluated for spawn run period, yield and biological efficiency. 42 isolates (10 di-and 32 monokaryotic isolates) were analyzed with RAPD genetic markers. Phenotypic characters and mating types of all the 42 isolates analyzed genetically were correlated with their genetic polymorphism data. The isolates showed very large diversity both at the phenotypic and the genotypic level. Available phenotypic and genotypic data can further help in the selection of monosporous isolates for developing interstrainal hybrids which can lead to better prospects for genetic improvement in different species of Pleurotus.

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Section: Identification Of Mating Types Of Single Basidiosporesmentioning

confidence: 96%

Molecular characterization and mating type analysis of oyster mushroom (Pleurotus spp.) using single basidiospores for strain improvement

Gupta

Reddy

Kotasthane

2010

World J Microbiol Biotechnol

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“…We used universally primed (UP)-PCR to detect DNA polymorphisms (Bulat et al 2000;Ikeda et al 2003;Ikeda et al 2005) with four primers (AS4, 5 0 -TGTGGGCGCTCGACAG; CA8G, 5 0 -CACACACACACACACAG; AS15inv, 5 0 -CATTGCTGGCGAAA TCGG; GTG, 5 0 -GTGGTGGTGGTGGTG). DNA amplification was performed in a 50 mL reaction mixture containing 1.25 U GoTaq DNA polymerase (Promega, Madison, WI, USA), 1Â PCR buffer, 2.5 mM MgCl 2 , 0.2 mM of each dNTP, 50 ng of template DNA, and 0.35 mM of the UP-PCR primer.…”

Section: Molecular Markers To Elucidate Paternitymentioning

confidence: 99%

Genetic analysis of barrage line formation during mycelial incompatibility in Rosellinia necatrix

et al. 2011

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“…In most Basidiomycetes, the proliferation of the dikaryon is accompanied by the formation of clamp cells that ensure the proper distribution of the genetically diverse nuclei. However, although widespread, clamps are not essential for the maintenance of a heterokaryotic mycelium; clampless dikaryons have been described in various cases (Swiezynski and Day, 1960a;Kemp, 1980;Salo et al, 1989), especially for various Uredinomycetes (for recent examples, see Frieders and McLaughlin, 2001;Ikeda et al, 2003). Similarly, it was generally believed that in U. maydis clamps are not required for dikaryotic growth.…”

Section: Clamp Formation In Ustilago Maydismentioning

confidence: 99%

The Clp1 Protein Is Required for Clamp Formation and Pathogenic Development ofUstilago maydis

et al. 2006

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In the phytopathogenic fungus Ustilago maydis, pathogenic development is controlled by a heterodimer of the two homeodomain proteins bE and bW, encoded by the b-mating-type locus. We have identified a b-dependently induced gene, clampless1 (clp1), that is required for the proliferation of dikaryotic filaments in planta. We show that U. maydis hyphae develop structures functionally equivalent to clamp cells that participate in the distribution of nuclei during cell division. In clp1 mutant strains, dikaryotic filaments penetrate the plant cuticle, but development is stalled before the first mitotic division, and the clamp-like structures are not formed. Although clp1 is immediately activated upon b-induction on the transcriptional level, nuclear-localized Clp1 protein is first observed at the stage of plant penetration prior to the first cell division. Induced expression of clp1 strongly interferes with b-dependent gene regulation and blocks b-dependent filament formation and b-dependent cell cycle arrest. We speculate that the Clp1 protein inhibits the activity of the bE/bW heterodimer to facilitate the cell cycle progression during hyphal growth.

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Mycelial incompatibility operative in pairings between single basidiospore isolates of Helicobasidium mompa

Cited by 18 publications

References 30 publications

Molecular characterization and mating type analysis of oyster mushroom (Pleurotus spp.) using single basidiospores for strain improvement

Molecular characterization and mating type analysis of oyster mushroom (Pleurotus spp.) using single basidiospores for strain improvement

Genetic analysis of barrage line formation during mycelial incompatibility in Rosellinia necatrix

The Clp1 Protein Is Required for Clamp Formation and Pathogenic Development ofUstilago maydis

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