Mycelium Polysaccharides from Termitomyces albuminosus Attenuate CCl4-Induced Chronic Liver Injury Via Inhibiting TGFβ1/Smad3 and NF-κB Signal Pathways

Abstract

A major fraction (MPT-W), eluted by deionized water, was extracted from mycelium polysaccharides of Termitomyces albuminosus (MPT), and its antioxidant, anti-fibrosis, and anti-inflammatory activities in CCl4-induced chronic liver injury mice, as well as preliminary characterizations, were evaluated. The results showed that MPT-W was a polysaccharide of α- and β-configurations containing xylose (Xyl), fucose (Fuc), mannose (Man), galactose (Gal), and glucose (Glc) with a molar ratio of 0.29:8.67:37.89:35.98:16.60 by gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared (FT-IR) spectroscopy. Its molecular weight (Mw), obtained by high-performance gel permeation chromatography (HPGPC), was 1.30 × 105 Da. The antioxidant assays in vitro showed that MPT-W displayed scavenging free-radical abilities. Based on the data of in vivo experiments, MPT-W could inhibit TGFβ1/Smad3 and NF-κB pathways; decrease the level and activity of cytochrome P4502E1 (CYP2E1), malonaldehyde (MDA) and serum enzyme; activate the HO-1/Nrf2 pathway; and increase antioxidant enzymes to protect the liver in CCl4-induced chronic liver injury mice. Therefore, MPT-W could be a potentially natural and functional resource contributing to antioxidant, hepatoprotective, and anti-inflammatory effects with potential health benefits.

A neutral polysaccharide from Ophiocordyceps lanpingensis restrains cisplatin‐induced nephrotoxicity

“…FT‐IR spectra of carbohydrates provide information on the primary structural characteristics of polysaccharides (Zhao et al., 2019). The FT‐IR spectrum of ONP is shown in Figure 1b.…”

The Protective Effect of Aspirin Eugenol Ester on Oxidative Stress to PC12 Cells Stimulated with H2O2 through Regulating PI3K/Akt Signal Pathway

“…Because of the high permeability of the plasma membrane to H 2 O 2 , H 2 O 2 -induced PC12 cells are generally considered to be an ideal cell model for studying neurodegenerative diseases [49][50][51]. Studies showed that the imbalance between free radical accumulation and antioxidant defense seems to be a link between cell death and the progression of neurodegenerative diseases [1,9,52]. ROS and the resulting oxidative stresses play an important role in apoptosis.…”

Bioactive Phytochemical Constituents of Wild Edible Mushrooms from Southeast Asia

“…It cannot be cultivated, because a symbiotic relationship with termites is necessary [229,230]. The mushroom has been reported to contain water-soluble polysaccharides with a great variety of biological activities, including antioxidant, anti-inflammatory, hepatoprotective, hypolipidemic activities [230][231][232][233][234]. In addition, T. albuminosus has been reported to contain many other bioactive components, such as chitin-glucan complex, alkaline protease, saponins, melanin, lipids and ergosterol; some of which possess analgesic and anti-inflammatory activities [235][236][237][238].…”

“…Polysaccharide (immunoenhancement [225,226] and anti-myelosuppressive activities [224]). Polysaccharide [230,[232][233][234],…”

“…Extract [239] --Polysaccharide (anti-inflammatory [234] and hepatoprotective effects [232,234]). Extract (analgesic and anti-inflammatory activities [236]).…”