Mycobacterium avium subspecies paratuberculosis

L, Li; Singh, S.V.; Bannantine, John P.; Kanjilal, Sagarika; Kapur, Vivek

doi:10.1007/978-3-540-74042-1_2

Cited by 4 publications

(1 citation statement)

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“…The emergence of drug resistance is a serious challenge for mastitis control due to their extensive use in the dairy industry, for example, through the dry cow therapy, contributes to the emergence of antimicrobial determinants in S. aureus , including development of multiple drug resistance ( Klibi et al, 2018 ; Liu et al, 2020 ; Omwenga et al, 2021 ; Gelalcha et al, 2022 ). Molecular epidemiology of staphylococcal species involved in IMI of dairy cattle have focused more on the use of multi-locus enzyme electrophoresis (MLEE), pulsed-field gel electrophoresis (PFGE), sequence-based typing schemes, such as multiple-locus sequence typing (MLST), multiple-locus VNTR (variable number of tandem repeats) analysis (MLVA), random amplification of polymorphic DNA (RAPD) analysis and staphylococcal protein A ( spa ) typing ( Li, 2008 ). Although these methods are helpful in genetic analysis, their resolution is often not strong enough to reveal genetic differences between strains.…”

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confidence: 99%

Comprehensive whole genome analysis of Staphylococcus aureus isolates from dairy cows with subclinical mastitis

Khasapane,

Nkhebenyane,

Mnisi

et al. 2024

Front. Microbiol.

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Staphylococcus species are the primary cause of mastitis in dairy cows across the world. Staphylococcus aureus has recently become a pathogen that is zoonotic and multidrug resistant. This study aimed to sequence whole genomes of 38 S. aureus isolates from 55 subclinical mastitis dairy cows of 7 small-scale farmers in the Free State Province, South Africa and document and their antimicrobial and virulence genes. The 38 isolates were grouped by the in silico multi-locus sequencing types (MLST) into seven sequence types (STs), that is (ST 97, 352, 152, 243) and three new STs (ST8495, ST8500, and ST8501). Thirty-three S. aureus isolates were divided into 7 core single-nucleotide polymorphism (SNP) clusters. Among the 9 distinct spa-types that were detected, Spa-types t2883 accounted for the majority of isolates at 12 (31.57%), followed by t416 with 11 (28.94%) and t2844 with 5 (13.15%). The data also revealed the identification of four (4) plasmids, with Rep_N (rep20) accounting for the majority of isolates with 17 (44.73%), followed by Inc18 (repUS5) with 2 (5.26%). These isolates included 11 distinct antimicrobial resistance genes and 23 genes linked to bacterial virulence. Surprisingly, no methicillin resistance associated genes were detected in these isolates. Genome data of the current study will contribute to understanding epidemiology S. aureus genotypes and ultimately aid in developing treatment and control plans to stop the spread of mastitis in the Free State province and South Africa as a whole.

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