Mycobiota and mycotoxins in fermented feed, wheat grains and corn grains in Southeastern Buenos Aires Province, Argentina

Roigé, M.B.; Aranguren, Sandra; Riccio, María Belén; Pereyra, Silvia; Soraci, Alejandro Luis; Tapia, María Ofelia

doi:10.1016/j.riam.2009.03.003

Cited by 44 publications

(33 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Beatriz Roigé in his research showed that Penicillium (42%), Fusarium (27%) and Alternaria (25%) were the most frequently genera recovered from wheat (11). In the present study, members of the genus Alternaria spp.…”

Section: Discussionsupporting

confidence: 56%

Mycoflora of Fungal Contamination in Wheat Storage (Silos) in Golestan Province, North of Iran

Joshaghani

Namjoo

Rostami

et al. 2013

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Cereal products are susceptible to mould damage during pre-and post-harvesting stages of the production. The regional specificity of Golestan province in the northern region of of Iran, with its high temperature and high relative humidity, acts as a leading factor for the growth of aflatoxin-producing fungi. It is well known that contamination of starch-based ingredients with mycotoxigenic fungi is a risk factor among the consumers due to its aflatoxins. Objectives: This survey was carried out to determine the extent of fungal contamination of wheat in three silos of Golestan province in Iran. Materials and Methods: 34 samples from three active silos were collected in order to clean the polyethylene bags. Wheat analyzed for fungal contamination and aflatoxins extracted by immunoaffinity column chromatography, and measured by HPLC method. Results: The most common moulds isolated were Alternaria spp. 26.7%, Aspergillus niger 21.4%, Fusarium spp. 17.8%, Aspergillus flavus 10.7%, Cladosporium spp. 10.7%, Penicillium spp. 8.9%, and Rhizopus spp. 3.5%. The screening of aflatoxin, B1, B2, G1 and G2 was carried out. 10(29.4%) samples of wheat had traces of aflatoxin, but in a level lower than the standard levels [Institute of Standards and Industrial Research of Iran (ISIR< 15 ng/g)].Conclusions: Despite the lower detected aflatoxin levels (lower than the ISIR level), the fungal contamination rate could not be neglected. Since the isolated mycotoxigenic fungi such as Aspergillus spp. and Fusarium spp. are important in food industry, it would be possible that the increased retention time of samples might have raised the detected contamination rate.

show abstract

Section: Discussionsupporting

confidence: 56%

Mycoflora of Fungal Contamination in Wheat Storage (Silos) in Golestan Province, North of Iran

Joshaghani

Namjoo

Rostami

et al. 2013

Jundishapur J Microbiol

View full text Add to dashboard Cite

show abstract

“…Roigé et al (2009) 5%). From the finding data of other researcher, it was concluded that the type of growth fungi on the agriculture commodities depends on the environmental condition, and it is known the Gaza weather differ than other countries.…”

Section: Discussionmentioning

confidence: 97%

Fungi and aflatoxins associated with wheat grains in Gaza governorates

Ghada¹,

Ahmed²,

Ferial³

et al. 2015

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Cereal and its products are susceptible to mold damage during pre-and post-harvesting stages of the production. This study was carried out to determine the extent of fungal contamination and the aflatoxins AFB 1 , AFB 2 , AFG 1 , AFG 2 levels of fifty wheat grain samples were collected randomly from different markets in the five Gaza governorates. The obtained results indicated that the most common molds isolated from different wheat grain samples were Aspergillus flavus 84%, Aspergillus parasiticus 72%, Fusarium oxysporum 64%, Aspergillus niger 48%, Alternaria alternate 36%, Penicillium 22%, Aspergillus ochraceus 20% and Aspergillus versicolor 4%. Forty one wheat grain samples which represented (82%) were contaminated with aflatoxins. Considering the high incidence of contamination by AFB 1 (80%) in Gaza city and 70% in both Khan Younis and Mid Zone governorates. The level of total aflatoxin AFs in North Gaza, Rafah, Khan Younis, Mid Zone, Gaza City were 8.62, 6.361, 4.187, 3.134 and 2.33 (ng/g), respectively. These levels of AFs are higher than the standard levels in North Gaza, Rafah and Khan Younis. The highest amount of aflatoxin B 1 were found in Mid Zone and North Gaza and their aflatoxins contamination were 2.51 and 2.31 ng/g, respectively.

show abstract

“…The most common genera are Aspergillus, Penicillium, Fusarium, and Alternaria isolated from wheat grain in Saudi Arabia (Al-Hazmi, 2010;Gashgari et al, 2010). The contamination of cereal grains by fungi is a worldwide problem, where there are several reports documenting the presence of these fungi in samples from the United States (Cleveland et al, 2003), Argentina (Roige et al, 2009), Spain (Sardiñas et al, 2011), and Algeria (Riba et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Aspergillus flavus and aflatoxin contamination of wheat grains from Saudi Arabia

Alwadai

Al‐Othman

Mahmoud³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Twelve species belonging to six fungal genera were found to be associated with wheat (Triticum aestivum L.) grain samples collected from three main regions in Saudi Arabia. The most common genera (average frequency) were Aspergillus (14.3%), Fusarium (29.1%), Penicillium (9.3%), and Alternaria (8.2%). Nineteen isolates of Aspergillus flavus were screened for their ability to produce aflatoxins using HPLC. Thirteen isolates produced aflatoxins ranging from 0.5 to 2.6 µg/kg. Inter-simple sequence repeats (ISSR), and random amplified polymorphic DNA (RAPD) molecular markers were used, with the aim of genetically characterizing strains of A. flavus to discriminate between aflatoxigenic and non-aflatoxigenic isolates. RAPD and ISSR analysis revealed a high level of genetic diversity in the A. flavus population, useful for genetic characterization. Clustering based on RAPD and ISSR dendograms was unrelated to geographic origin. RAPD and ISSR markers were not suitable to discriminate aflatoxigenic and nonaflatoxigenic isolates, but ISSR primers were better compared to RAPD.

show abstract

Mycobiota and mycotoxins in fermented feed, wheat grains and corn grains in Southeastern Buenos Aires Province, Argentina

Cited by 44 publications

References 32 publications

Mycoflora of Fungal Contamination in Wheat Storage (Silos) in Golestan Province, North of Iran

Mycoflora of Fungal Contamination in Wheat Storage (Silos) in Golestan Province, North of Iran

Fungi and aflatoxins associated with wheat grains in Gaza governorates

Molecular characterization of Aspergillus flavus and aflatoxin contamination of wheat grains from Saudi Arabia

Contact Info

Product

Resources

About