Mycofabrication of bioactive silver nanoparticle: Photo catalysed synthesis and characterization to attest its augmented bio-efficacy

Patil, H.B.; Nithin, K.S.; Sachhidananda, S.; Siddaramaiah,; Chandrashekara, K. T.; Kumar, Baban

doi:10.1016/j.arabjc.2016.07.009

Cited by 20 publications

(3 citation statements)

References 41 publications

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“…Nanoparticles are multidimensional materials that range from 1 to 100 nm in size (Ali, Qiang, Ilahi, Adnan, & Sajjad, 2018). Nanomaterials can be synthesized as, that is, chemically (Vasanth Patil et al, 2019), biologically using fungal species (Ghomi et al, 2019), and plant species (Khatua et al, 2020a). The physical synthesis of nanoparticles presents high energy consumption while the chemical production yields some toxic materials as byproducts (Behravan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Biological synthesis of silver nanoparticles using animal blood, their preventive efficiency of bacterial species, and ecotoxicity in common carp fish

Kakakhel

Feng

et al. 2021

Microscopy Res & Technique

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Possible high biodeterioration of the microorganisms due to their metabolic pathway and activities on stone materials causes solemn problems in cultural heritage. Different kinds of laboratory-scale methods have been used for the reduction of microbial growth, that is, chemical, mechanical, and physical, which are cost-effective and not ecofriendly. In the current study, an ecofriendly approach utilizing silver nanoparticles were synthesized using sheep blood serum. Transmission electron microscopy results have confirmed the spherical and well dispersed silver nanoparticles with an average size of 32.49 nm, while energy dispersive X-ray has shown the abundance of silver nanoparticles. The efficiency against bacterial species was verified through laboratory-scale testing. The strong antibacterial activity was confirmed when B-AgNPs was tested against different bacterial species isolated from the Beishiku Cave Temple. The largest zone of inhibition was measured 26.48 ± 0.14 mm against Sphingomonas sp. while the smallest zone of inhibition measured was 9.70 ± 0.27 mm against Massilia sp. Moreover, these ecofriendly B-AgNPs were tested for daily based dose in different concentrations (0.03, 0.06, and 0.09 mg/L) against common carp fish for a long exposure (20 days) and 6.5% fatality was found. The highest lethal concentration (LC 50 ) for fish (0.61 ± 0.09 mg/L). No doubt, the laboratory scale applications have revealed the best results with minute toxicity in fish. Therefore, sheep serum should be continued to synthesize silver nanoparticles on a large scale. A strict monitoring system should be developed for the synthesis and application of AgNPs.

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Section: Introductionmentioning

confidence: 99%

Biological synthesis of silver nanoparticles using animal blood, their preventive efficiency of bacterial species, and ecotoxicity in common carp fish

Kakakhel

Feng

et al. 2021

Microscopy Res & Technique

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“…On the contrary, the biosynthesis of AgNPs by the actinomycete Sinomonas mesophila MPKL 26 was achieved only after exposure to sunlight [75]. Additionally, the sunlight-assisted biosynthesis of AgNPs by various fungal and plant extracts is well-documented [76][77][78][79][80][81].…”

Section: Discussionmentioning

confidence: 99%

Biogenic Silver Nanoparticles Produced by Soil Rare Actinomycetes and Their Significant Effect on Aspergillus-derived mycotoxins

et al. 2023

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The current investigation addressed the green synthesis of silver nanoparticles (AgNPs) using newly isolated silver-resistant rare actinomycetes, Glutamicibacter nicotianae SNPRA1 and Leucobacter aridicollis SNPRA2, and investigated their impact on the mycotoxigenic fungi Aspergillus flavus ATCC 11498 and Aspergillus ochraceus ATCC 60532. The formation of AgNPs was evidenced by the reaction’s color change to brownish and the appearance of the characteristic surface plasmon resonance. The transmission electron microscopy of biogenic AgNPs produced by G. nicotianae SNPRA1 and L. aridicollis SNPRA2 (designated Gn-AgNPs and La-AgNPs, respectively) revealed the generation of monodispersed spherical nanoparticles with average sizes of 8.48 ± 1.72 nm and 9.67 ± 2.64 nm, respectively. Furthermore, the XRD patterns reflected their crystallinity and the FTIR spectra demonstrated the presence of proteins as capping agents. Both bioinspired AgNPs exhibited a remarkable inhibitory effect on the conidial germination of the investigated mycotoxigenic fungi. The bioinspired AgNPs caused an increase in DNA and protein leakage, suggesting the disruption of membrane permeability and integrity. Interestingly, the biogenic AgNPs completely inhibited the production of total aflatoxins and ochratoxin A at concentrations less than 8 μg/mL. At the same time, cytotoxicity investigations revealed the low toxicity of the biogenic AgNPs against the human skin fibroblast (HSF) cell line. Both biogenic AgNPs exhibited feasible biocompatibility with HSF cells at concentrations up to 10 μg/mL and their IC50 values were 31.78 and 25.83 μg/mL for Gn-AgNPs and La-AgNPs, respectively. The present work sheds light on the antifungal prospect of the biogenic AgNPs produced by rare actinomycetes against mycotoxigenic fungi as promising candidates to combat mycotoxin formation in food chains at nontoxic doses.

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“…For Ag NPs synthesis, about 30 ml of cell-free supernatant and 20 ml silver nitrate (AgNO 3 ) solution were incubated at 28°C and 150 rpm for 72 h. After the incubation period, the solution was observed for visual color change. The flasks turned from opaque white to dark brown that indicated the presence of bio synthesized Ag NPs ( Zonooz and Salouti, 2011 ; Patil et al., 2019 ).…”

Section: Methodsmentioning

confidence: 99%

First identification of potato tuber rot caused by Penicillium solitum, its silver nanoparticles synthesis, characterization and use against harmful pathogens

Shah,

Shan,

Baig

et al. 2023

Front. Plant Sci.

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Potato is one of the highly consumed vegetable crop grown in different regions across Pakistan that is affected by fungal diseases. The current research was conducted to identify fungal pathogen causing mold-like disease of potato in Khyber Pakhtunkhwa (KP), Pakistan. For molecular identification and characterization of the fungal disease; potato tuber samples were collected followed by culturing on potato dextrose agar (PDA). Based on morphological features, the pathogen was identified as a Penicillium species. This result was obtained in 45 different isolates from potato tubers. Molecular identification was done using β-tubulin primers and ITS5 sequencing of 13 different isolates that releveled 98% homology with BLAST (GenBank accession no. KX958076) as Penicillium solitum (GenBank accession nos. ON307317; ON307475 and ON310801). Phylogenetic tree was constructed that showed Penicillium solitum prevalence along with Penicillium polonicum and Penicillium citrinum on potato tubers. Based on this, Penicillium solitum based silver nanoparticles (Ag NPs) were synthesized and characterized using UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray (EDX) and field emission scanning electron microscopy (FE SEM). UV-analysis showed a characteristic peak at 410 nm confirming synthesis of Penicillium solitum based Ag NPs. This was further confirmed by XRD followed by EDX and SEM that showed face cubic crystal structure with Ag as major constituent of 18 nm formed spherical Ag NPs. FTIR showed band stretching of O-H, N-O and C-H of biological origin. Similarly, Penicillium solitum based Ag NPs presented strong anti-bacterial and anti-fungal activity at 0.5 level of significance LSD. According to our knowledge, this is the first report of Penicillium solitum identification in Pakistan, its Ag NPs synthesis and characterization to be used against pathogens of agricultural significance.

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Mycofabrication of bioactive silver nanoparticle: Photo catalysed synthesis and characterization to attest its augmented bio-efficacy

Cited by 20 publications

References 41 publications

Biological synthesis of silver nanoparticles using animal blood, their preventive efficiency of bacterial species, and ecotoxicity in common carp fish

Biological synthesis of silver nanoparticles using animal blood, their preventive efficiency of bacterial species, and ecotoxicity in common carp fish

Biogenic Silver Nanoparticles Produced by Soil Rare Actinomycetes and Their Significant Effect on Aspergillus-derived mycotoxins

First identification of potato tuber rot caused by Penicillium solitum, its silver nanoparticles synthesis, characterization and use against harmful pathogens

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