Mycoplasma hyopneumoniae detection by PCR in naturally infected finishing pigs

Takeuti, Karine Ludwig; Michaelsen, Taís Regina; Sabedot, Carla; Nagae, Ricardo Yuiti; Forner, Ricardo; Mazzarollo, Angela; Barcellos, David Emílio Santos Neves de; Pieters, Maria

doi:10.1016/j.mimet.2022.106475

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…Reported clinical signs were characterized by dyspnea, anorexia, lethargy, and an increment in >50% mortality. The presence of PCV2 [ 24 ], PRRSV [ 25 ], CSFV [ 26 ], PPV [ 27 ], swine influenza virus (AIV) [ 28 ], and Mycoplasma hyopneumoniae [ 29 ] infection was ruled out based on specific PCR detection. Specifically, the isolate was obtained via a single passage in a PCV1- and PCV2-free porcine kidney cell line (PK-15, ATCC ® CCL-33™, Hong Kong, China).…”

Section: Methodsmentioning

confidence: 99%

Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China

Wang

et al. 2023

Veterinary Sciences

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Porcine circovirus 3 (PCV3) is an emerging virus first discovered in the United States in 2015, and since then, PCV3 has been found in many regions of the world, including America, Asia, and Europe. Although several PCV3 investigations have been carried out, there is a lack of knowledge regarding the pathogenicity of PCV3, mostly due to the limited number of PCV3 isolates that are readily available. In this study, PCV3-DB-1 was isolated in PK-15 cells and characterized in vitro. Electron microscopy revealed the presence of PCV-like particles, and in situ hybridization RNA analysis demonstrated the replication of PCV3 in PK-15 cell culture. Based on phylogenetic analysis of PCV3 isolates from the Heilongjiang province of China, PCV3-DB-1 with 24 alanine and 27 lysine in the Cap protein was originally isolated and determined to belong to the clade PCV3a.

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Section: Methodsmentioning

confidence: 99%

Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China

Wang

et al. 2023

Veterinary Sciences

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A diagnostic approach to confirm Mycoplasma hyopneumoniae “Day zero” for pathogen eradication

Sponheim,

Alvarez,

Fano

et al. 2023

Preventive Veterinary Medicine

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Acclimation of replacement gilts to Mycoplasma hyopneumoniae: a case study of fogging with an aerosol inoculum

Nagae,

Barcellos,

Ulguim

et al. 2023

Anim. Prod. Sci.

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Context Mycoplasma hyopneumoniae causes enzootic pneumonia, predisposing pigs to infections with other respiratory pathogens. The main control measure is to reduce piglet exposure at lactation, which can be achieved by gilts’ acclimation prior to their entrance to the farms. One of the acclimation strategies is aerosol exposure with a positive inoculum by using a fogger. However, studies on its efficacy in gilts and their litters are lacking in the literature. Aim The aim of this case study was to assess the efficacy fogging with a positive inoculum of M. hyopneumoniae to expose negative gilts in an acclimation program. Moreover, the infection dynamics of M. hyopneumoniae were assessed in their piglets from lactation to slaughter. Methods The trial was performed in two phases. In Phase 1, a total of 34 and 107 gilts was selected from Farms A and B respectively, and then exposed to the inoculum through a fogger. In Phase 2, a subsample of 74 gilts from Farm B was followed to their first farrowing and 263 piglets born to those gilts were sampled from 15 to 170 days of age, and at slaughter, lung lesions were evaluated. Key results In Phase 1, the prevalence of positive gilts at 28 days post-exposure (dpe) was 100% and 98.1% in Farms A and B respectively. In Phase 2, 10.8% of gilts remained positive at 180 dpe and 0.8% of piglets were positive at 15 days of age (day) and 28.1% at 60 days, suggesting a possible vertical transmission. Conclusion The use of fogging with a lung homogenate positive for M. hyopneumoniae successfully acclimated negative gilts. However, it did not avoid the presence of positive gilts at farrowing and the detection of the bacterium in their progeny by polymerase chain reaction. Implications The exposure of gilts to M. hyopneumoniae at 150 days of age was successfully achieved by fogging. However, consideration should be given to exposing replacement gilts of a younger age in order to reduce the odds of detecting positive gilts at first farrowing.

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Mycoplasma hyopneumoniae detection by PCR in naturally infected finishing pigs

Cited by 4 publications

References 8 publications

Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China

Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China

A diagnostic approach to confirm Mycoplasma hyopneumoniae “Day zero” for pathogen eradication

Acclimation of replacement gilts to Mycoplasma hyopneumoniae: a case study of fogging with an aerosol inoculum

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