Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro

Deeney, Alannah S.; Maglennon, Gareth; Chapat, Ludivine; Crussard, Steve; Jolivet, Edmond; Rycroft, Andrew N.

doi:10.1186/s13567-019-0667-6

Cited by 15 publications

(13 citation statements)

References 42 publications

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“…IFN-γ promotes the activation and proliferation of T cells and macrophages, which play vital roles in host defenses in the lungs. Previous studies showed that macrophage dysfunction was induced by Mycoplasma spp., including M. bovis, and promoted persistent infection (2,(66)(67)(68). Immune reactivation by combination therapy of anti-PD-L1 mAb with COX-2 inhibitor could help in the development of potential methods for the control of M. bovis infection.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of PD-L1 Expression by Prostaglandin E2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection

et al. 2020

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Bovine mycoplasmosis caused by Mycoplasma bovis results in pneumonia and mastitis in cattle. We previously demonstrated that the programmed death 1 (PD-1)/PD-ligand 1 (PD-L1) pathway is involved in immune dysfunction during M. bovis infection and that prostaglandin E 2 (PGE 2 ) suppressed immune responses and upregulated PD-L1 expression in Johne's disease, a bacterial infection in cattle. In this study, we investigated the role of PGE 2 in immune dysfunction and the relationship between PGE 2 and the PD-1/PD-L1 pathway in M. bovis infection. In vitro stimulation with M. bovis upregulated the expressions of PGE 2 and PD-L1 presumably via Toll-like receptor 2 in bovine peripheral blood mononuclear cells (PBMCs). PGE 2 levels of peripheral blood in infected cattle were significantly increased compared with those in uninfected cattle. Remarkably, plasma PGE 2 levels were positively correlated with the proportions of PD-L1 + monocytes in M. bovis-infected cattle. Additionally, plasma PGE 2 production in infected cattle was negatively correlated with M. bovis-specific interferon (IFN)-γ production from PBMCs. These results suggest that PGE 2 could be one of the inducers of PD-L1 expression and could be involved in immunosuppression during M. bovis infection. In vitro blockade Goto et al.PGE 2 -Induced Immunodysfunction During Bovine Mycoplasmosis assays using anti-bovine PD-L1 antibody and a cyclooxygenase 2 inhibitor significantly upregulated the M. bovis-specific IFN-γ response. Our study findings might contribute to the development of novel therapeutic strategies for bovine mycoplasmosis that target PGE 2 and the PD-1/PD-L1 pathway.

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Section: Discussionmentioning

confidence: 99%

Upregulation of PD-L1 Expression by Prostaglandin E2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection

et al. 2020

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“…Furthermore, specific IgG diffusing from the blood into the lung tissue or produced locally in the BALT could opsonize M. hyopneumoniae , resulting in phagocytosis by macrophages and neutrophils [ 53 ]. However, Deeney et al [ 54 ] recently reported that addition of convalescent porcine sera did not enhance engulfment of M. hyopneumoniae by alveolar macrophages in vitro.…”

Section: Interactions Of M Hyopneumoniae With the Hostmentioning

confidence: 99%

Perspectives for improvement of Mycoplasma hyopneumoniae vaccines in pigs

Maes

Boyen

Devriendt

et al. 2021

Vet Res

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Mycoplasma hyopneumoniae (M. hyopneumoniae) is one of the primary agents involved in the porcine respiratory disease complex, economically one of the most important diseases in pigs worldwide. The pathogen adheres to the ciliated epithelium of the trachea, bronchi, and bronchioles, causes damage to the mucosal clearance system, modulates the immune system and renders the animal more susceptible to other respiratory infections. The pathogenesis is very complex and not yet fully understood. Cell-mediated and likely also mucosal humoral responses are considered important for protection, although infected animals are not able to rapidly clear the pathogen from the respiratory tract. Vaccination is frequently practiced worldwide to control M. hyopneumoniae infections and the associated performance losses, animal welfare issues, and treatment costs. Commercial vaccines are mostly bacterins that are administered intramuscularly. However, the commercial vaccines provide only partial protection, they do not prevent infection and have a limited effect on transmission. Therefore, there is a need for novel vaccines that confer a better protection. The present paper gives a short overview of the pathogenesis and immune responses following M. hyopneumoniae infection, outlines the major limitations of the commercial vaccines and reviews the different experimental M. hyopneumoniae vaccines that have been developed and tested in mice and pigs. Most experimental subunit, DNA and vector vaccines are based on the P97 adhesin or other factors that are important for pathogen survival and pathogenesis. Other studies focused on bacterins combined with novel adjuvants. Very few efforts have been directed towards the development of attenuated vaccines, although such vaccines may have great potential. As cell-mediated and likely also humoral mucosal responses are important for protection, new vaccines should aim to target these arms of the immune response. The selection of proper antigens, administration route and type of adjuvant and carrier molecule is essential for success. Also practical aspects, such as cost of the vaccine, ease of production, transport and administration, and possible combination with vaccines against other porcine pathogens, are important. Possible avenues for further research to develop better vaccines and to achieve a more sustainable control of M. hyopneumoniae infections are discussed.

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“…Gram-negative and Gram-positive bacteria have at least 6 reported secretion/translocation systems [126]. The general secretory (Sec) pathway comprises an essential, ubiquitous and universal export machinery for most proteins that translocate through (soluble secretome) or integrate into (insoluble secretome) the cell membrane [127]. In mycoplasmas, however, the Sec secretory pathway is apparently incomplete, as some genes coding for components of this pathway were not found in their genomes.…”

Section: Protein Secretion Of M Hyopneumoniae and Its Impact On Pathmentioning

confidence: 99%

Pathogenicity & virulence ofMycoplasma hyopneumoniae

et al. 2020

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Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro

Cited by 15 publications

References 42 publications

Upregulation of PD-L1 Expression by Prostaglandin E2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection

Upregulation of PD-L1 Expression by Prostaglandin E2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection

Perspectives for improvement of Mycoplasma hyopneumoniae vaccines in pigs

Pathogenicity & virulence ofMycoplasma hyopneumoniae

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