“…This is not surprising, as different samples were used and, even within the same sample, metabarcoding reveals more diversity due to the fact that only a single region of each genome is being targeted, allowing for deeper sequencing. While many of the metabarcoding and metagenomic reads were classified as microbiota typically found in seawater, there were several species present that are typically found in large mammals, particularly ungulates (Corbeil et al 1985, Friis et al 1991, Williams and Barker 2008). While nominally commensal in healthy animals, microbes residing on the skin or in the respiratory tract may become pathogenic or more invasive in stressed or immunosuppressed individuals.…”
“…This is not surprising, as different samples were used and, even within the same sample, metabarcoding reveals more diversity due to the fact that only a single region of each genome is being targeted, allowing for deeper sequencing. While many of the metabarcoding and metagenomic reads were classified as microbiota typically found in seawater, there were several species present that are typically found in large mammals, particularly ungulates (Corbeil et al 1985, Friis et al 1991, Williams and Barker 2008). While nominally commensal in healthy animals, microbes residing on the skin or in the respiratory tract may become pathogenic or more invasive in stressed or immunosuppressed individuals.…”
“…If the above examinations are negative, examinations for parasitic infestations, such as parasite IgG in serum and/or examinations for helminth eggs in stool and the pleural effusion are considered. After confirmation of negative findings from these examinations, with an increasing amount of eosinophilic pleural effusion, thoracoscopy can be performed to make a diagnosis of malignancy and infections such as tuberculosis, as reported previously [ 4 , 22 , 23 ], although its use may be limited, because thoracoscopy can be performed in few institutions, and it is an invasive examination for the patient. Fig.…”
A 37-year-old man with fever, cough, and dyspnea with no medical history developed an eosinophilic pleural effusion and blood eosinophilia. No evidence of malignancy or pathogens was detected in the pleural effusion, and the pleural specimen obtained by thoracoscopy showed eosinophilic infiltration with inflammatory granulation tissue without fibrinoid necrosis or malignant cells. Since a myeloproliferative disorder was also excluded, the diagnosis was idiopathic eosinophilic pleurisy. Corticosteroid treatment was started and then slowly tapered, and the eosinophilic pleural effusion resolved. Considering the various etiologies of eosinophilic pleurisy, a practical clinical approach to the investigation and diagnosis of eosinophilic pleurisy is presented.
“…Todas as características culturais apresentadas pelo M. hyosynoviae estão de acordo com o descrito na literatura [5,6]. A baixa freqüência de isolamento deste agente provavelmente deve-se a fato de que o M. hyosynoviae desaparece das articulações afetadas após um período de três semanas [14].…”
Section: Discussionunclassified
“…O Mycoplasma hyosynoviae é um dos agentes causadores de artrite em suínos na recria e terminação sendo que esta afecção pode persistir até o momento do abate [12,13], gerando condenações e aproveitamento condicional das carcaças. As tonsilas dos animais infectados são o principal reservatório do M. hyosynoviae [6] podendo os suínos ser portadores deste agente em suas tonsilas sem manifestarem artrite e sem desenvolverem anticorpos [8].…”
O Mycoplasma hyosynoviae é um dos agentes causadores de artrite em suínos em fase de recria e terminação. Esta infecção provoca atraso no crescimento, gastos com tratamento e condenações de carcaças. O presente trabalho teve como objetivo padronizar a técnica de isolamento e identificação do M. hyosynoviae e investigar a presença deste agente na articulação de suínos artríticos no matadouro. Procedeu-se o exame microbiológico das articulações de 50 carcaças de suínos recentemente abatidos e julgados artríticos pelo Serviço de Inspeção Federal. Utilizaram-se técnicas de isolamento adequadas para o crescimento dos principais agentes artritogênicos dos suínos com especial atenção ao isolamento do M. hyosynoviae. Dos 50, 40 (80%) eram assépticas, de sete (14%) foi isolado o Erysipelothrix rhusiopathiae e de três (6%), o M. hyosynoviae, sendo este último isolado pela primeira vez no Brasil. O M. hyosynoviae apresentou crescimento rápido nos meios de cultivos líquidos e sólidos. O crescimento deste agente foi evidenciado pela alcalinização do meio, com conseqüente alteração da cor original salmão para cor vinho. Dos três isolamentos de campo, em dois foi observado o crescimento nos tubos 24 horas após e em um, cinco dias após a semeadura. Conclui-se que o M. hyosynoviae cresce abundantemente e com rapidez nos meios líquidos e sólidos enriquecidos com arginina e mucina.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
