Mycoplasma infection in the uterus of early postpartum dairy cows and its relation to dystocia and endometritis

Ghanem, Mohamed Elshabrawy; Higuchi, Hidetoshi; Tezuka, Erisa; Ito, Hideki; Devkota, Bhimsen; Izaike, Yoshiaki; Osawa, Takeshi

doi:10.1016/j.theriogenology.2012.09.027

Cited by 31 publications

(34 citation statements)

References 23 publications

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“…Mycoplasma californicum appears to be the second most common cause of mycoplasma-associated disease [8] and is associated with mastitis in adults [9, 10] and arthritis and pneumonia in calves [11]. Mycoplasma bovigenitalium can be isolated from the reproductive tract of cows, and has been associated with vulvovaginitis and infertility [12], as well as dystocia and endometritis [13]. …”

Section: Introductionmentioning

confidence: 99%

Comparison of culture and a multiplex probe PCR for identifying Mycoplasma species in bovine milk, semen and swab samples

et al. 2017

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Mycoplasma spp. are a major cause of mastitis, arthritis and pneumonia in cattle, and have been associated with reproductive disorders in cows. While culture is the traditional method of identification the use of PCR has become more common. Several investigators have developed PCR protocols to detect M. bovis in milk, yet few studies have evaluated other sample types or other important Mycoplasma species. Therefore the objective of this study was to develop a multiplex PCR assay to detect M. bovis, M. californicum and M. bovigenitalium, and evaluate its analytical performance against traditional culture of bovine milk, semen and swab samples. The PCR specificity was determined and the limit of detection evaluated in spiked milk, semen and swabs. The PCR was then compared to culture on 474 field samples from individual milk, bulk tank milk (BTM), semen and swab (vaginal, preputial, nose and eye) samples. Specificity analysis produced appropriate amplification for all M. bovis, M. californicum and M. bovigenitalium isolates. Amplification was not seen for any of the other Mollicutes or eubacterial isolates. The limit of detection of the PCR was best in milk, followed by semen and swabs. When all three Mycoplasma species were present in a sample, the limit of detection increased. When comparing culture and PCR, overall there was no significant difference in the proportion of culture and PCR positive samples. Culture could detect significantly more positive swab samples. No significant differences were identified for semen, individual milk or BTM samples. PCR identified five samples with two species present. Culture followed by 16S-23S rRNA sequencing did not enable identification of more than one species. Therefore, the superior method for identification of M. bovis, M. californicum and M. bovigenitalium may be dependent on the sample type being analysed, and whether the identification of multiple target species is required.

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Section: Introductionmentioning

confidence: 99%

Comparison of culture and a multiplex probe PCR for identifying Mycoplasma species in bovine milk, semen and swab samples

et al. 2017

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“…Numerous studies have demonstrated the presence of Mycoplasma species in the genital tracts of diseased and healthy cows (26)(27)(28). Genital infections caused by mycoplasmas are generally asymptomatic but have been reported to be the most important cause of infertility (26,28).…”

Section: Discussionmentioning

confidence: 99%

“…Genital infections caused by mycoplasmas are generally asymptomatic but have been reported to be the most important cause of infertility (26,28). In a previous study, M. bovis and M. bovigenitalium were isolated from genital samples of 76 infertile and 86 healthy cows at rates of 18.7% and 15.1%, respectively (26), while only M. bovigenitalium (7.4%) was detected in metritic uterus samples in another study, which aimed to determine 7 different species of Mycoplasma (28). In the present study, 16.2% and 27% of mycoplasmas isolated from uterus samples were M. bovis and M. bovigenitalium, respectively.…”

Section: Discussionmentioning

confidence: 99%

Mycoplasma infections in dairy cattle farms in Turkey

Sayın¹,

Sakmanoğlu²,

Uçan³

et al. 2016

Turk J Vet Anim Sci

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“…Clear mucus was scored 0; mucus containing flecks of white pus was scored 1; discharge composed of less than 50% white mucopurulent material was scored 2; discharge composed of more than 50% white or yellow pus was scored 3; and discharge composed of more than 50% white or yellow pus with a fetid odor was scored 4 [5]. Uterine swab samples (n = 128) were collected from all of the cows at W5 and W7 using cytobrushes (Puritan Medical Products, Guilford, ME, USA) adapted for use in cattle, as described by Ghanem et al [17]. The ovarian status was assessed by evaluation of the plasma progesterone levels.…”

Section: Methodsmentioning

confidence: 99%

“…The slides were then transported to the laboratory within 3 h and stained using the Diff-Quik stain (Sysmex, Kobe, Japan) for 20 sec. The percentage of neutrophils (PMN) was evaluated as described by Ghanem et al [17]. The threshold values for PMN indicating subclinical endometritis were ≥ 6% at W5 and ≥ 4% at W7 [20].…”

Section: Methodsmentioning

confidence: 99%

Correlation of blood metabolite concentrations and body condition scores with persistent postpartum uterine bacterial infection in dairy cows

Ghanem

Tezuka

Sasaki

et al. 2016

Journal of Reproduction and Development

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To analyze the relationship of blood metabolite concentrations and body condition score (BCS) with persistent bacterial uterine infection, specifically that caused by Trueperella pyogenes and anaerobic bacteria, uterine bacteriological swabs (n = 128) were collected from 64 Holstein cows at 5 (W5) and 7 (W7) weeks postpartum, and the percentage of neutrophils in the endometrium was evaluated. Blood glucose, total cholesterol (T-cho), blood urea nitrogen (BUN), non-esterified fatty acid (NEFA), and β-hydroxybutyric acid concentrations were analyzed at 3 weeks (W-3) and 1 week (W-1) prepartum and W3, W5, and W7 postpartum. BCS were evaluated at W-3, W3, and W7. Blood glucose concentrations at W-3 and W-1 in cows with persistent bacterial infection were lower (P = 0.05) than in the rest of the cows. Total BUN concentrations in cows with persistent bacterial infection were lower (P < 0.01) than those in other cows, although the association between the pre or postpartum time and status of infection was not significant. Total NEFA concentrations in cows with persistent bacterial infection were similar to those in uninfected cows and cows positive for infection at W5 but not W7. Total BCS in cows with persistent bacterial infection were lower (P < 0.01) than those in cows positive for infection at both W5 but not W7 and W7 but not W5; however, the association between the pre or postpartum time and status of infection was not significant. Glucose concentrations at W-3 and W-1 negatively correlated with persistent bacterial infection at W5 and W7 (P < 0.01). BUN concentrations at W3 (P < 0.01), W5 (P < 0.05), and W7 (P < 0.05) and BCS at W3 (P < 0.01) negatively correlated with persistent postpartum bacterial infection. Decreased prepartum blood glucose concentrations might be an important risk factor for persistent postpartum bacterial uterine infection in dairy cows.

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Mycoplasma infection in the uterus of early postpartum dairy cows and its relation to dystocia and endometritis

Cited by 31 publications

References 23 publications

Comparison of culture and a multiplex probe PCR for identifying Mycoplasma species in bovine milk, semen and swab samples

Comparison of culture and a multiplex probe PCR for identifying Mycoplasma species in bovine milk, semen and swab samples

Mycoplasma infections in dairy cattle farms in Turkey

Correlation of blood metabolite concentrations and body condition scores with persistent postpartum uterine bacterial infection in dairy cows

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