Mycoplasma membrane potentials determined by potential-sensitive fluorescent dyes

Schummer, Ulrich; Schiefer, Hans-Gerd; Gerhardt, Ursula

doi:10.1007/bf02605881

Cited by 21 publications

(8 citation statements)

References 11 publications

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“…The membrane potential was measured using an optical probe DiSC,(5) which responds to the membrane potential of the cell [8]. Briefly, after incubation with glucose, erythrocytes were suspended at a haematocrit of 0.2% in buffered saline, containing 10 mmol 1-' Tris-HC1, pH 7.4 and 150 mmol I-' (KCl+NaCl), with K' concentrations ranging from 50 to 140 mmol 1-'.…”

mentioning

confidence: 99%

Human red blood cell membrane potential and fluidity in glucose solutions

Заводник

Piasecka

Szosland

et al. 1997

Scandinavian Journal of Clinical and Laboratory Investigation

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This study aimed to investigate the membrane potential and fluidity changes of human red blood cells subjected to isotonic glucose solutions. For the control erythrocytes a membrane potential value of -10.1 +/- 1.8 mV was obtained, whereas erythrocyte membranes in diabetic cells were hyperpolarized, with a potential of -13.9 +/- 2.3 mV. Incubation of both types of red blood cells with increasing glucose concentrations resulted in a substantial hyperpolarization of the cell membranes. Glucose solutions in water had a much stronger effect on membrane potential than glucose dissolved in phosphate-buffered saline. Red blood cell membrane fluidity measurements using the fluorescent label TMADPH did not reveal any significant changes upon incubation with glucose.

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mentioning

confidence: 99%

Human red blood cell membrane potential and fluidity in glucose solutions

Заводник

Piasecka

Szosland

et al. 1997

Scandinavian Journal of Clinical and Laboratory Investigation

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“…We conclude that Ap is kept remarkably constant at zip = -123 mV + 16°70 over a wide range of pHext values. Its magnitude is distinctly higher than that of other mycoplasmal species [7][8][9].…”

Section: Determination Of Aphmentioning

confidence: 72%

“…The membrane potential zi¢ was determined using a potential-sensitive fluorescent carbocyanine dye [6][7][8][9]. With this method the membrane potential is compared with a K + diffusion potential induced by addition of the K+-specific ionophore valinomycin.…”

Section: R Tziph]/f = Zi~ -(Z-ziph)mentioning

confidence: 99%

Transmembrane proton‐motive potential of Spiroplasma foricola

Schummer

Schiefer

1987

FEBS Letters

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In Spiroplasma.floricola, the transmembrane proton-motive potential zip was studied. It is composed of a transmembrane electric potential difference, ziq;, and a transmembrane proton gradient, zipH, according to zip = 3~9 -(Z.ZIpH). Using a potential-sensitive carbocyanine dye and 5,5'-dimethyl[2-14C]oxazolidine -2,4-dione as probes, zi~ and zipH were measured at different [H +] of the medium, and zip was calculated to be remarkably constant at 123 mV + 16% over a wide range of external pH values. Inhibition experiments indicated that it is generated by a membrane-bound, electrogenic, proton-translocating ATPase.

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“…At low K + concentrations the transmembrane potential reaches higher negative values in the presence of valinomycin than in the situation without valinomycin according to Schummer et al 19 In the presence of high K § levels, valinomycin causes a decrease of the transmembrane potential (less negatively charged inside). As shown in Table I copper uptake in the presence of DMP responded strongly to variations of the membrane potential induced by valinomycin under the described conditions.…”

Section: Test For Electrogenic Couplingmentioning

confidence: 89%

Investigation into the mechanism of copper uptake by Mycoplasma gallisepticum in the prescence of 2,9-dimethyl-1,10-phenanthroline

Gaisser¹,

Goot²,

Stouthamer³

et al. 1987

Pharmaceutisch Weekblad Scientific Edition

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In the presence of copper certain 2,2'-bipyridyls show antimycoplasmal activity, whereas copper itself causes a toxic effect. In this paper results are presented to elucidate the mechanism of copper uptake in the presence of 2,9-dimethyl-1,10-phenanthroline. The time course of copper and/or ligand uptake under the applied conditions is consistent with a carrier transport mechanism in which 2,9-dimethyl-1,10-phenanthroline operates as a carrier for copper ions. The influence of valinomycin on copper uptake indicates that the transmembrane potential is not the driving force in the carrier process.

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Mycoplasma membrane potentials determined by potential-sensitive fluorescent dyes

Cited by 21 publications

References 11 publications

Human red blood cell membrane potential and fluidity in glucose solutions

Human red blood cell membrane potential and fluidity in glucose solutions

Transmembrane proton‐motive potential of Spiroplasma foricola

Investigation into the mechanism of copper uptake by Mycoplasma gallisepticum in the prescence of 2,9-dimethyl-1,10-phenanthroline

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