Mycoplasmas and cell cultures

Stanbridge, Eric J.

doi:10.1128/br.35.2.206-227.1971

Cited by 116 publications

(8 citation statements)

References 180 publications

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“…The highest dilution that lowered the pH of half of the fibroblast cultures was designated as 1 color-changing unit. The change in pH indicated by the change in color of the phenol red indicator present in the culture medium is attributable to the fermentation of glucose by the growing mycoplasma in the cultures (2,23).…”

Section: Methodsmentioning

confidence: 99%

Lymphocyte activation by various Mycoplasma strains and species

Naot¹,

Tully²,

Ginsburg³

1977

Infect Immun

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The capacity of various mycoplasma strains and species to induce lymphocyte transformation in vitro was studied. Of six strains of Mycoplasma pulmonis studied, five displayed mitogenic activity with rat lymphocytes. Among those M. pulmonis strains, our MP15 isolate and the Negroni strain exhibited particularly potent mitogenic capacity. The murine mycoplasmas M. neurolyticum and M. arthritidis shared this mitogenicity for rat lymphocytes. However, the human mycoplasmas M. fermentans, M. pneumoniae, M. hominis, M. orale, and Acholeplasma laidlawii did not activate rat lymphocytes. Lymphocytes obtained from germfree rats were activated to the same extent as those from animals bred under conventional conditions. The mitogenic potency exhibited by mycoplasma was not restricted to infective microorganisms, and preparations of killed mycoplasma particles exerted an extensive lymphocyte transformation. The data show that the mitogenic activity of mycoplasmas is not confined to a single mycoplasma isolate and that it acts in a nonspecific manner.

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Section: Methodsmentioning

confidence: 99%

Lymphocyte activation by various Mycoplasma strains and species

Naot¹,

Tully²,

Ginsburg³

1977

Infect Immun

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“…Mycoplasmas can affect virus propagation [50], and the effect is dependent upon the type of the mycoplasma strain. While some mycoplasmas have no effect on virus yield can increase virus yields with arginine-utilizing mycoplasmas can decrease the titer of vaccinia virus [51].…”

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine

Safini

Elmejdoub

Bamouh

et al. 2022

Viruses

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Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP). Lumpy skin disease (LSD) is a viral disease of cattle caused by lumpy skin disease virus (LSDV). LSD and CBPP are both transboundary diseases spreading in the same areas of Africa and Asia. A combination vaccine to control CBPP and LSD offers significant value to small-scale livestock keepers as a single administration. Access to a bivalent vaccine may improve vaccination rates for both pathogens. In the present study, we evaluated the LSDV/CBPP live combined vaccine by testing the generation of virus neutralizing antibodies, immunogenicity, and safety on target species. In-vitro assessment of the Mycoplasma effect on LSDV growth in cell culture was evaluated by infectious virus titration and qPCR during 3 serial passages, whereas in-vivo interference was assessed through the antibody response to vaccination. This combined Mmm/LSDV vaccine could be used to protect cattle against both diseases with a single vaccination in the endemic countries. There were no adverse reactions detected in this study and inoculated cattle produced high levels of specific antibodies starting from day 7 post-vaccination, suggesting that this combination vaccine is both safe and effective.

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“…The contamination of B lymphoblastoid cell lines with mycoplasmas is a problem shared with other tissue culture systems (8,15). Some strains of mycoplasma can contaminate cultures without causing gross cellular damage (1), but will drastically alter host cell metabolism (16).…”

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confidence: 99%

Effects of mycoplasma contamination on immunoglobulin biosynthesis by human B lymphoblastoid cell lines

Hendershot¹,

Levitt²

1985

Infect Immun

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The synthesis and secretion of immunoglobulin M (IgM), as well as the relative ratio of membrane and secretory mu heavy chain (mu m and mu s, respectively), were evaluated in mycoplasma-contaminated B lymphoblastoid cell lines. The ratio of mu m to mu s was drastically lowered in infected cultures, and mu s chains could now combine with light chains. A 50 to 100% increase in IgM synthesis occurred in contaminated cultures, and small amounts of IgM were detectable in the culture media. These molecules possessed mu chains typical of secreted IgM. Reexpression of mu on the surface of B lymphoblastoid cells was substantially delayed in mycoplasma-contaminated cultures. Thus, mycoplasma contamination alters the synthesis and expression of a specific differentiated gene product (immunoglobulin) in B cell lines; such changes could significantly affect the interpretation of data on immunoglobulin synthesis by B cells with different phenotypes. This system may also provide a means of studying how mycoplasma infection alters specific gene expression in B cell lines.

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Mycoplasmas and cell cultures

Cited by 116 publications

References 180 publications

Lymphocyte activation by various Mycoplasma strains and species

Lymphocyte activation by various Mycoplasma strains and species

Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine

Effects of mycoplasma contamination on immunoglobulin biosynthesis by human B lymphoblastoid cell lines

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